BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0512 (594 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S... 109 2e-25 SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch... 102 5e-23 SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 26 3.6 SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.8 SPAC27D7.08c |||DUF890 family protein|Schizosaccharomyces pombe|... 25 6.3 SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar... 25 6.3 SPBC609.04 |caf5||spermine family transmembrane transporter Caf5... 25 8.3 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 8.3 SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 25 8.3 SPAC17C9.16c ||SPAC9E9.16|MFS family transmembrane transporter M... 25 8.3 >SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|Schizosaccharomyces pombe|chr 2|||Manual Length = 141 Score = 109 bits (263), Expect = 2e-25 Identities = 53/91 (58%), Positives = 66/91 (72%) Frame = +1 Query: 235 FPQTQDVEPPRHPKYPRKSLPKRNRMDAYNIIKFPLTSEAAMKKIEDNNTLVFIVHTSAN 414 F + + +E R PKY RKS+P +R+D Y II P+ SE+AMKKIED+NTLVF VH AN Sbjct: 31 FRRPKTLELARKPKYARKSVPHASRLDEYKIIVNPINSESAMKKIEDDNTLVFHVHLKAN 90 Query: 415 XHHIKAAVKKLYDINVAKVNTLIRPDGKKKA 507 IK AVKKLY ++ K+NTLIRP+G KKA Sbjct: 91 KFTIKNAVKKLYSVDAVKINTLIRPNGTKKA 121 Score = 38.3 bits (85), Expect = 8e-04 Identities = 19/33 (57%), Positives = 21/33 (63%) Frame = +2 Query: 152 VTKALKAQRKVVKGEHGKRVRKIRNSVHFRRPK 250 V KA AQ+ V KG H K RK+R S FRRPK Sbjct: 3 VGKAKGAQKTVQKGIHNKVARKVRTSTTFRRPK 35 Score = 29.9 bits (64), Expect = 0.29 Identities = 12/23 (52%), Positives = 19/23 (82%) Frame = +3 Query: 498 EESVVRLARDYDALDVANKIGII 566 +++ V+L+ D DALDVAN+IG + Sbjct: 119 KKAFVKLSADADALDVANRIGFL 141 >SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosaccharomyces pombe|chr 2|||Manual Length = 141 Score = 102 bits (244), Expect = 5e-23 Identities = 50/91 (54%), Positives = 63/91 (69%) Frame = +1 Query: 235 FPQTQDVEPPRHPKYPRKSLPKRNRMDAYNIIKFPLTSEAAMKKIEDNNTLVFIVHTSAN 414 F + + ++ R PKY RKS+ R+D Y II P+ SE+AMKKIED+NTLVF VH AN Sbjct: 31 FRRPKTLQLSRKPKYARKSVAHAPRLDEYKIIVNPINSESAMKKIEDDNTLVFHVHLKAN 90 Query: 415 XHHIKAAVKKLYDINVAKVNTLIRPDGKKKA 507 IK AV+KLY + K+NTLIRP+G KKA Sbjct: 91 KFTIKEAVRKLYSVEPVKINTLIRPNGTKKA 121 Score = 37.9 bits (84), Expect = 0.001 Identities = 18/33 (54%), Positives = 21/33 (63%) Frame = +2 Query: 152 VTKALKAQRKVVKGEHGKRVRKIRNSVHFRRPK 250 V KA AQ+ V KG H K +K+R S FRRPK Sbjct: 3 VAKAKGAQKTVQKGIHNKVAKKVRTSTTFRRPK 35 Score = 29.9 bits (64), Expect = 0.29 Identities = 12/23 (52%), Positives = 19/23 (82%) Frame = +3 Query: 498 EESVVRLARDYDALDVANKIGII 566 +++ V+L+ D DALDVAN+IG + Sbjct: 119 KKAFVKLSADADALDVANRIGFL 141 >SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 836 Score = 26.2 bits (55), Expect = 3.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = +1 Query: 52 SQNCQTQASSFQIEDCTEAQKDWD*GTKKS 141 S+NCQ + +++C EA D D G K+ Sbjct: 479 SENCQKLSKQIPLDECNEALFDDDNGDYKA 508 >SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 490 Score = 25.8 bits (54), Expect = 4.8 Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 1/50 (2%) Frame = +1 Query: 196 TWEESTENSQLCAFPQTQDVEPPRHPKYPRKSLPKRNR-MDAYNIIKFPL 342 +W TENS + + P KY RK+ K N +D+ + K P+ Sbjct: 385 SWHGDTENSVKQSLASPSEASLPNLSKYSRKNAKKLNEWLDSLD-FKLPI 433 >SPAC27D7.08c |||DUF890 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 385 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = +1 Query: 472 NTLIRPDGKKKASYDSLEIMTHWTLPTKLAS 564 N LIRP + Y +++ +WTL +L + Sbjct: 261 NELIRPSSTSLSKYFPHKVLQNWTLDPELCA 291 >SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.4 bits (53), Expect = 6.3 Identities = 14/36 (38%), Positives = 17/36 (47%), Gaps = 2/36 (5%) Frame = +1 Query: 178 EGCKR*TWEESTENSQLCAFPQ-TQDV-EPPRHPKY 279 E CK + C F Q++ EPPRHPKY Sbjct: 330 EPCKNWQISGTCRYGSKCQFAHGNQELKEPPRHPKY 365 >SPBC609.04 |caf5||spermine family transmembrane transporter Caf5|Schizosaccharomyces pombe|chr 2|||Manual Length = 531 Score = 25.0 bits (52), Expect = 8.3 Identities = 7/10 (70%), Positives = 9/10 (90%) Frame = -2 Query: 74 AWVWQFWLLL 45 +W WQFWLL+ Sbjct: 238 SWRWQFWLLM 247 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 25.0 bits (52), Expect = 8.3 Identities = 9/51 (17%), Positives = 24/51 (47%) Frame = +1 Query: 310 MDAYNIIKFPLTSEAAMKKIEDNNTLVFIVHTSANXHHIKAAVKKLYDINV 462 + A+++ +F E ++ +DN T I H + + + A +++ + Sbjct: 1090 LQAFDLSRFEFIKEIFLELYDDNETNASIAHQISTQNGLDATETSFFELQI 1140 >SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 599 Score = 25.0 bits (52), Expect = 8.3 Identities = 8/27 (29%), Positives = 16/27 (59%) Frame = -2 Query: 287 FLGYLGCLGGSTSWVCGNAQSCEFSVL 207 ++GY+ C+ G + +C NA + V+ Sbjct: 427 YVGYISCVAGMGAMICVNATTSMSKVM 453 >SPAC17C9.16c ||SPAC9E9.16|MFS family transmembrane transporter Mfs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 531 Score = 25.0 bits (52), Expect = 8.3 Identities = 7/10 (70%), Positives = 9/10 (90%) Frame = -2 Query: 74 AWVWQFWLLL 45 +W WQFWLL+ Sbjct: 238 SWRWQFWLLM 247 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,178,298 Number of Sequences: 5004 Number of extensions: 41276 Number of successful extensions: 125 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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