BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0489 (639 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g37150.4 68414.m04644 holocarboxylase synthetase 2 (HCS2.d) i... 31 0.49 At1g37150.3 68414.m04643 holocarboxylase synthetase 2 (HCS2.d) i... 31 0.49 At1g37150.2 68414.m04642 holocarboxylase synthetase 2 (HCS2.d) i... 31 0.49 At1g37150.1 68414.m04641 holocarboxylase synthetase 2 (HCS2.d) i... 31 0.49 At1g11950.1 68414.m01381 transcription factor jumonji (jmjC) dom... 28 4.5 At2g22300.1 68415.m02646 ethylene-responsive calmodulin-binding ... 28 6.0 >At1g37150.4 68414.m04644 holocarboxylase synthetase 2 (HCS2.d) identical to holocarboxylase synthetase hcs2.d [Arabidopsis thaliana] GI:19698373; contains non-consensus GG acceptor splice sites; contains Pfam profile PF03099: Biotin/lipoate A/B protein ligase family; contains TIGRfam profile TIGR00121: biotin--acetyl-CoA-carboxylase ligase; Length = 286 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/48 (39%), Positives = 28/48 (58%) Frame = +2 Query: 119 GGILP*TTRRSMESLPPWRYAQKMDNIRPCTSACLFSLLQPVCVPTNV 262 GGIL +T RS + L +DN +P T CL ++L+ VC P+N+ Sbjct: 141 GGILCTSTYRSRKFLVSVGVGLNVDNEQPTT--CLNAVLKDVCPPSNL 186 >At1g37150.3 68414.m04643 holocarboxylase synthetase 2 (HCS2.d) identical to holocarboxylase synthetase hcs2.d [Arabidopsis thaliana] GI:19698373; contains non-consensus GG acceptor splice sites; contains Pfam profile PF03099: Biotin/lipoate A/B protein ligase family; contains TIGRfam profile TIGR00121: biotin--acetyl-CoA-carboxylase ligase; Length = 297 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/48 (39%), Positives = 28/48 (58%) Frame = +2 Query: 119 GGILP*TTRRSMESLPPWRYAQKMDNIRPCTSACLFSLLQPVCVPTNV 262 GGIL +T RS + L +DN +P T CL ++L+ VC P+N+ Sbjct: 184 GGILCTSTYRSRKFLVSVGVGLNVDNEQPTT--CLNAVLKDVCPPSNL 229 >At1g37150.2 68414.m04642 holocarboxylase synthetase 2 (HCS2.d) identical to holocarboxylase synthetase hcs2.d [Arabidopsis thaliana] GI:19698373; contains non-consensus GG acceptor splice sites; contains Pfam profile PF03099: Biotin/lipoate A/B protein ligase family; contains TIGRfam profile TIGR00121: biotin--acetyl-CoA-carboxylase ligase; Length = 329 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/48 (39%), Positives = 28/48 (58%) Frame = +2 Query: 119 GGILP*TTRRSMESLPPWRYAQKMDNIRPCTSACLFSLLQPVCVPTNV 262 GGIL +T RS + L +DN +P T CL ++L+ VC P+N+ Sbjct: 184 GGILCTSTYRSRKFLVSVGVGLNVDNEQPTT--CLNAVLKDVCPPSNL 229 >At1g37150.1 68414.m04641 holocarboxylase synthetase 2 (HCS2.d) identical to holocarboxylase synthetase hcs2.d [Arabidopsis thaliana] GI:19698373; contains non-consensus GG acceptor splice sites; contains Pfam profile PF03099: Biotin/lipoate A/B protein ligase family; contains TIGRfam profile TIGR00121: biotin--acetyl-CoA-carboxylase ligase; Length = 214 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/48 (39%), Positives = 28/48 (58%) Frame = +2 Query: 119 GGILP*TTRRSMESLPPWRYAQKMDNIRPCTSACLFSLLQPVCVPTNV 262 GGIL +T RS + L +DN +P T CL ++L+ VC P+N+ Sbjct: 101 GGILCTSTYRSRKFLVSVGVGLNVDNEQPTT--CLNAVLKDVCPPSNL 146 >At1g11950.1 68414.m01381 transcription factor jumonji (jmjC) domain-containing protein contains Pfam domain, PF02373: jmjC domain; non-consensus TG acceptor splice site at exon boundary 79262 Length = 880 Score = 28.3 bits (60), Expect = 4.5 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = +3 Query: 114 DLVVFYPEQQGALWSPFLPGDMPK 185 D +V Y E GALW F D+PK Sbjct: 718 DEIVVYDETSGALWDIFKREDVPK 741 >At2g22300.1 68415.m02646 ethylene-responsive calmodulin-binding protein, putative (SR1) identical to partial sequence of ethylene-induced calmodulin-binding protein GI:11545505 from [Arabidopsis thaliana]; contains Pfam profiles PF03859: CG-1 domain, PF00612: IQ calmodulin-binding motif, and PF00023: Ankyrin repeat Length = 1032 Score = 27.9 bits (59), Expect = 6.0 Identities = 12/37 (32%), Positives = 21/37 (56%) Frame = -3 Query: 301 TEENGVHSKRPTFDIGGDTHGLKKRKQTGARAWADII 191 T+ + + K T + G T+GLK RK ++ W +I+ Sbjct: 258 TDSSIMVDKSKTINSPGVTNGLKNRKSIDSQTWEEIL 294 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,431,966 Number of Sequences: 28952 Number of extensions: 353330 Number of successful extensions: 834 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 799 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 834 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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