Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0479
         (695 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...    74   2e-14
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF...    71   1e-13
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    29   0.84 
SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   5.9  
SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4 |Schiz...    25   7.8  

>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score = 73.7 bits (173), Expect = 2e-14
 Identities = 35/94 (37%), Positives = 57/94 (60%)
 Frame = +1

Query: 13  TFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFT 192
           TF++VE+FW ++++I   S L    DY+ F++G+RP WED  NK GG+W     K +   
Sbjct: 67  TFNSVEEFWGIHNNINPASSLPIKSDYSFFREGVRPEWEDVHNKTGGKWAFQ-NKGRGGN 125

Query: 193 DLDRFWLDVVLLLIGENFENSMRFVVLLLMSDQK 294
            LD  WL  VL  IGE  + + + V+ ++++ +K
Sbjct: 126 ALDEMWLTTVLAAIGETLDPTGQEVMGVVINMRK 159



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 2/70 (2%)
 Frame = +3

Query: 258 EICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGI--HGKIGFQVHRDTMVKH 431
           E+ G V+N+R    ++A+WT     +   +EIG + K+ L +     I F  H D+    
Sbjct: 149 EVMGVVINMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSSKSG 208

Query: 432 SSATKNLYTV 461
           S+  K   +V
Sbjct: 209 STRAKTRMSV 218


>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
           4F complex subunit |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 243

 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 33/79 (41%), Positives = 47/79 (59%)
 Frame = +1

Query: 13  TFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFT 192
           +F TVE+FW ++  I   S L    DY+ F +GIRP WED  N  GG+W  + + K + +
Sbjct: 93  SFKTVEEFWGIFKTISKASMLPAKSDYSYFLKGIRPEWEDPQNMNGGKW--AYQSKHKGS 150

Query: 193 DLDRFWLDVVLLLIGENFE 249
           +LD  WL +VL  IGE  +
Sbjct: 151 NLDELWLYMVLAAIGETLD 169



 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 22/65 (33%), Positives = 32/65 (49%), Gaps = 2/65 (3%)
 Frame = +3

Query: 258 EICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGK--IGFQVHRDTMVKH 431
           E+ G V N+R    +IA+WT +   +    +IG + KE LGI  K  I +  H D+    
Sbjct: 174 EVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDSSKAG 233

Query: 432 SSATK 446
           S   K
Sbjct: 234 SMRAK 238


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
 Frame = +2

Query: 380 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 475
           D  Q+W FP TQ++ N    FC+Q   H L+LAL
Sbjct: 98  DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131


>SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 527

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = +1

Query: 112 IRPMWEDDANKMGGRWLIS-LEKKQRFTDLDRFWLDVV 222
           +  +WE   N   G +L   +EKK  F  +DR+W  V+
Sbjct: 161 VAALWEQ-FNIFHGSYLFDEIEKKSSFEIIDRWWTSVL 197


>SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 674

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = +3

Query: 231 NW*KFREFDEICGAVVNVR 287
           NW ++  F+E CG   NVR
Sbjct: 206 NWLRWARFEEECGNAANVR 224


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,666,956
Number of Sequences: 5004
Number of extensions: 52239
Number of successful extensions: 156
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)