Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0479
(695 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18040.1 68417.m02685 eukaryotic translation initiation facto... 76 2e-14
At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identi... 71 7e-13
At5g35620.2 68418.m04252 eukaryotic translation initiation facto... 70 1e-12
At5g35620.1 68418.m04251 eukaryotic translation initiation facto... 70 1e-12
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 68 5e-12
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 68 7e-12
At1g05140.1 68414.m00517 membrane-associated zinc metalloproteas... 30 1.7
>At4g18040.1 68417.m02685 eukaryotic translation initiation factor
4E 1 / eIF-4E1 / mRNA cap-binding protein 1 (EIF4E1)
identical to SP|O23252 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-4F 25 kDa subunit) (eIF-4F P26 subunit)
{Arabidopsis thaliana}
Length = 235
Score = 76.2 bits (179), Expect = 2e-14
Identities = 35/80 (43%), Positives = 50/80 (62%)
Frame = +1
Query: 13 TFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFT 192
TF TVE+FW LY+++K PS+L G D+ FK I P WED GG+W ++ K++
Sbjct: 91 TFSTVEEFWSLYNNMKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKS-- 148
Query: 193 DLDRFWLDVVLLLIGENFEN 252
D+ WL +L LIGE F++
Sbjct: 149 --DKSWLYTLLALIGEQFDH 166
Score = 68.5 bits (160), Expect = 4e-12
Identities = 34/73 (46%), Positives = 45/73 (61%)
Frame = +3
Query: 240 KFREFDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDT 419
+F DEICGAVVN+R K ++I+IWT +A + A + IGK+ KE L + IGF +H D
Sbjct: 163 QFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDA 222
Query: 420 MVKHSSATKNLYT 458
K KN YT
Sbjct: 223 K-KLDRNAKNAYT 234
>At5g18110.1 68418.m02126 novel cap-binding protein (nCBP) identical
to novel cap-binding protein nCBP [Arabidopsis thaliana]
GI:3108209; contains Pfam profile PF01652: Eukaryotic
initiation factor 4E
Length = 221
Score = 70.9 bits (166), Expect = 7e-13
Identities = 34/80 (42%), Positives = 45/80 (56%)
Frame = +1
Query: 16 FDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTD 195
F TVE FW Y H+ S L D FK GIRP+WED AN GG+W+I K
Sbjct: 75 FSTVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRFSK----VV 130
Query: 196 LDRFWLDVVLLLIGENFENS 255
RFW D++L L+G+ +++
Sbjct: 131 SARFWEDLLLALVGDQLDDA 150
Score = 37.1 bits (82), Expect = 0.011
Identities = 19/68 (27%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +3
Query: 255 DEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGI-HGKIGFQVHRDTMVKH 431
D ICGAV++VR D I++W +A A + + +K L + H + D ++
Sbjct: 151 DNICGAVLSVRFNEDIISVWNRNASDHQAVMGLRDSIKRHLKLPHAYVMEYKPHDASLRD 210
Query: 432 SSATKNLY 455
+S+ +N +
Sbjct: 211 NSSYRNTW 218
>At5g35620.2 68418.m04252 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 167
Score = 70.1 bits (164), Expect = 1e-12
Identities = 34/87 (39%), Positives = 51/87 (58%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 RHETTFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGR--WLISLE 174
R TFDTVEDFW L+ I S+L + +FK G+ P WED GG+ W+++
Sbjct: 51 RKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTAN 110
Query: 175 KKQRFTDLDRFWLDVVLLLIGENFENS 255
+K+ LD+ WL+ ++ LIGE F+ +
Sbjct: 111 RKEA---LDKGWLETLMALIGEQFDEA 134
Score = 36.7 bits (81), Expect = 0.015
Identities = 16/28 (57%), Positives = 21/28 (75%), Gaps = 2/28 (7%)
Frame = +3
Query: 240 KFREFDEICGAVVNVRP--KVDKIAIWT 317
+F E DEICG V +VRP K DK+++WT
Sbjct: 130 QFDEADEICGVVASVRPQSKQDKLSLWT 157
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 124 WEDDANKMGGRWLISLEKKQRFTDLDRFW 210
W D+ +K G W SL K F ++ FW
Sbjct: 35 WFDNQSKKGAAWGASLRKAYTFDTVEDFW 63
>At5g35620.1 68418.m04251 eukaryotic translation initiation factor
4E 2 / eIF-4E2 / mRNA cap-binding protein 2 (EIF4E2)
identical to SP|O04663 Eukaryotic translation initiation
factor 4E (eIF-4E) (eIF4E) (mRNA cap-binding protein)
(eIF-(iso)4F 25 kDa subunit) (eIF-(ISO)4F P28 subunit)
(eIF4Eiso protein) {Arabidopsis thaliana}
Length = 198
Score = 70.1 bits (164), Expect = 1e-12
Identities = 34/87 (39%), Positives = 51/87 (58%), Gaps = 2/87 (2%)
Frame = +1
Query: 1 RHETTFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGR--WLISLE 174
R TFDTVEDFW L+ I S+L + +FK G+ P WED GG+ W+++
Sbjct: 51 RKAYTFDTVEDFWGLHETIFQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTAN 110
Query: 175 KKQRFTDLDRFWLDVVLLLIGENFENS 255
+K+ LD+ WL+ ++ LIGE F+ +
Sbjct: 111 RKEA---LDKGWLETLMALIGEQFDEA 134
Score = 54.8 bits (126), Expect = 5e-08
Identities = 28/62 (45%), Positives = 37/62 (59%), Gaps = 2/62 (3%)
Frame = +3
Query: 240 KFREFDEICGAVVNVRP--KVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHR 413
+F E DEICG V +VRP K DK+++WT + + IGKK KE L + KI F H
Sbjct: 130 QFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHD 189
Query: 414 DT 419
D+
Sbjct: 190 DS 191
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 124 WEDDANKMGGRWLISLEKKQRFTDLDRFW 210
W D+ +K G W SL K F ++ FW
Sbjct: 35 WFDNQSKKGAAWGASLRKAYTFDTVEDFW 63
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 68.1 bits (159), Expect = 5e-12
Identities = 34/74 (45%), Positives = 46/74 (62%)
Frame = +3
Query: 240 KFREFDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDT 419
+F + DEICGAV+N R + D+I++WT +A + A + IGK+ KE LG + IGF VH D
Sbjct: 168 QFDQGDEICGAVLNFRARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDA 227
Query: 420 MVKHSSATKNLYTV 461
A K YTV
Sbjct: 228 KTLDRDA-KRRYTV 240
Score = 65.3 bits (152), Expect = 4e-11
Identities = 31/79 (39%), Positives = 44/79 (55%)
Frame = +1
Query: 13 TFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFT 192
TF T+E+FW LY++I P++ G D FK I P WED GG+W + K
Sbjct: 96 TFGTIEEFWSLYNNIHPPTKWVSGADLYCFKDKIEPKWEDPICANGGKWSMMFPK----A 151
Query: 193 DLDRFWLDVVLLLIGENFE 249
L+ WL+ +L L+GE F+
Sbjct: 152 TLECNWLNTLLALVGEQFD 170
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 67.7 bits (158), Expect = 7e-12
Identities = 34/74 (45%), Positives = 45/74 (60%)
Frame = +3
Query: 240 KFREFDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGKIGFQVHRDT 419
+F + DEICGAV+N R + D+I++WT A + A + IGK+ KE LG + IGF VH D
Sbjct: 213 QFDQGDEICGAVLNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDA 272
Query: 420 MVKHSSATKNLYTV 461
A K YTV
Sbjct: 273 KTLDRDA-KRRYTV 285
Score = 64.9 bits (151), Expect = 5e-11
Identities = 30/79 (37%), Positives = 44/79 (55%)
Frame = +1
Query: 13 TFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQGIRPMWEDDANKMGGRWLISLEKKQRFT 192
TF T+E+FW LY++I P++ G D FK I P WED GG+W + +
Sbjct: 141 TFATIEEFWSLYNNIHPPTKWVSGSDLYCFKDKIEPKWEDPICANGGKWTMFFPR----A 196
Query: 193 DLDRFWLDVVLLLIGENFE 249
L+ WL+ +L L+GE F+
Sbjct: 197 TLESNWLNTLLALVGEQFD 215
>At1g05140.1 68414.m00517 membrane-associated zinc metalloprotease,
putative similar to Hypothetical zinc metalloprotease
All3971 (SP:Q8YQ64) [strain PCC 7120] {Anabaena sp.};
Similar to Synechocystis hypothetical protein
(gb|D90908);contains Pfam PF00595: PDZ domain (Also
known as DHR or GLGF); contains TIGRFAM TIGR00054:
membrane-associated zinc metalloprotease, putative
Length = 441
Score = 29.9 bits (64), Expect = 1.7
Identities = 23/58 (39%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = -1
Query: 338 MLLHCISCPNSNFI-YFWSDINNSTTNLIEFSKFSPIKRR--TTSNQNLSKSVKRCFF 174
MLL+ S P S+ I +F SD NN T+N SK K T SN +L + R F+
Sbjct: 1 MLLNISSSPISHRIPHFLSDFNNPTSNFPPKSKTHLPKSNLSTLSNHSLYGTKNRAFY 58
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,569,618
Number of Sequences: 28952
Number of extensions: 260438
Number of successful extensions: 621
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 595
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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