BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0473 (691 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 29 0.48 SPCC1223.10c |eaf1||RNA polymerase II transcription elongation f... 28 1.1 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 28 1.5 SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 27 2.6 SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 5.9 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 26 5.9 SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 26 5.9 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 5.9 SPAC3G9.09c |tif211||translation initiation factor eIF2 alpha su... 25 7.8 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 29.5 bits (63), Expect = 0.48 Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 2/38 (5%) Frame = +1 Query: 547 LCKVIVDFKI--IFSQTYNNVNSNLFKWCYFLVLRNSD 654 L V V K+ IFS ++ V++N++ W Y V +SD Sbjct: 525 LSTVFVKLKLQGIFSSSFQQVSNNMYSWIYDHVFSSSD 562 >SPCC1223.10c |eaf1||RNA polymerase II transcription elongation factor SpEAF|Schizosaccharomyces pombe|chr 3|||Manual Length = 251 Score = 28.3 bits (60), Expect = 1.1 Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 4/66 (6%) Frame = +3 Query: 48 VIYNTPNGDRTLK-IEEIARKNGDTDKAISNSNPGNAIPSGTTNR---GNVTSNVETTIL 215 +I+N TL+ I+EIAR N + +S + P NAI ++ TS T Sbjct: 81 LIFNAKTKTFTLEHIDEIARLNALRNPKVSKTVPSNAITQSDNSQISESKSTSQSAVTTN 140 Query: 216 DLRRKD 233 RRK+ Sbjct: 141 STRRKE 146 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = +3 Query: 36 TSVGVIYNTPNGDRTLKIEEIARKNGDTD--KAISNSNPGNAIPS 164 T G NTP DR+L I +++R + + +S SNP AI S Sbjct: 446 TRPGSATNTPQSDRSLDINDLSRSSSYSRHLSHVSLSNPDFAIGS 490 >SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 27.1 bits (57), Expect = 2.6 Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 10/67 (14%) Frame = +3 Query: 75 RTLKIEEIARKNGDTDKAISNSNPGNAIPSGTTNRGNVTSNVETTIL----------DLR 224 + ++I +NGD + SN + NAI + TT++GN N E T+L D+R Sbjct: 453 KEIQILRHTSQNGDQE---SNESSKNAITTKTTDKGN---NKENTMLNDGSTAPAKNDIR 506 Query: 225 RKDNTNN 245 NTNN Sbjct: 507 NVINTNN 513 >SPBC17D1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/63 (25%), Positives = 31/63 (49%) Frame = +3 Query: 57 NTPNGDRTLKIEEIARKNGDTDKAISNSNPGNAIPSGTTNRGNVTSNVETTILDLRRKDN 236 +TP D+TL+ NG+ + +N S + +V+S++ T LD +++ + Sbjct: 268 STPT-DQTLRPPNPPAANGNATPVTAVNNVSAVADSPQSTGSSVSSSLPTLSLDFKQEYS 326 Query: 237 TNN 245 NN Sbjct: 327 GNN 329 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/36 (36%), Positives = 20/36 (55%) Frame = +3 Query: 18 NSNTDTTSVGVIYNTPNGDRTLKIEEIARKNGDTDK 125 NSNTDTT+ G + + + T E + K+ D+ K Sbjct: 318 NSNTDTTTSGEVSAQSSTNSTSSEESTSVKDEDSSK 353 >SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1562 Score = 25.8 bits (54), Expect = 5.9 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +3 Query: 72 DRTLKIEEIARKNGDTDKAISNSNPGNAIPSGTTNRGNVT 191 D+++ E ARK+ + DKAI ++ + PS T +R +++ Sbjct: 886 DKSVTKAEQARKSIEIDKAIIRNSQSTSRPSLTASRPSLS 925 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 25.8 bits (54), Expect = 5.9 Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 1/73 (1%) Frame = +1 Query: 475 FKNIILVKTETTPQQCCSLCFS-WKLCKVIVDFKIIFSQTYNNVNSNLFKWCYFLVLRNS 651 FKN +L E P +L + ++L + ++ Y S+ + CYF +S Sbjct: 4018 FKNNLLNLLELLPTMTHTLPHNRFRLFFFLSWLHATLAEIYCFTCSSWKEPCYF---DDS 4074 Query: 652 DFYFGLAIGCRML 690 DFYFG I C +L Sbjct: 4075 DFYFGTKILCNIL 4087 >SPAC3G9.09c |tif211||translation initiation factor eIF2 alpha subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 306 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +2 Query: 47 GYI*YSKRRQNPQDRRNCQKK 109 GYI SKRR +P+D C+++ Sbjct: 81 GYIDLSKRRVSPEDVVKCEER 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,653,847 Number of Sequences: 5004 Number of extensions: 50413 Number of successful extensions: 132 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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