Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0458
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 113 6e-26
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 89 2e-18
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 89 2e-18
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 87 1e-17
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 66 2e-11
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 31 0.42
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 31 0.42
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 5.1
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 6.8
At3g63180.1 68416.m07097 expressed protein 27 6.8
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 27 9.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 113 bits (273), Expect = 6e-26
Identities = 49/81 (60%), Positives = 64/81 (79%)
Frame = +1
Query: 13 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 192
VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDHW+++
Sbjct: 747 VLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMS 806
Query: 193 GDPCEPQSKPYNVVQETRKRK 255
DP EP ++ +V + RKRK
Sbjct: 807 SDPLEPGTQASVLVADIRKRK 827
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 89.0 bits (211), Expect = 2e-18
Identities = 40/65 (61%), Positives = 48/65 (73%)
Frame = +1
Query: 13 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 192
VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P
Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 922
Query: 193 GDPCE 207
GDP +
Sbjct: 923 GDPLD 927
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 89.0 bits (211), Expect = 2e-18
Identities = 40/65 (61%), Positives = 48/65 (73%)
Frame = +1
Query: 13 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 192
VL+RRRGHV + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P
Sbjct: 863 VLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 922
Query: 193 GDPCE 207
GDP +
Sbjct: 923 GDPLD 927
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 86.6 bits (205), Expect = 1e-17
Identities = 39/65 (60%), Positives = 48/65 (73%)
Frame = +1
Query: 13 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 192
VL+RRRG+V + GTP +IVKA+LPV ESFGF DLR +T GQAF VFDHW ++P
Sbjct: 849 VLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVP 908
Query: 193 GDPCE 207
GDP +
Sbjct: 909 GDPLD 913
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 65.7 bits (153), Expect = 2e-11
Identities = 29/63 (46%), Positives = 39/63 (61%)
Frame = +1
Query: 13 VLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLP 192
VL+RRR + +E G+ +F V AY+PV+ESFGF +LR T G A V HW++L
Sbjct: 896 VLSRRRARILKEEMQEGSSLFTVHAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLE 955
Query: 193 GDP 201
DP
Sbjct: 956 EDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 31.5 bits (68), Expect = 0.42
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +1
Query: 10 GVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 150
G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 678 GDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 31.5 bits (68), Expect = 0.42
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +1
Query: 10 GVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQ 150
G +N+R+G + Q ++ A +P+N FG++ LRS T G+
Sbjct: 678 GDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYSTSLRSMTQGK 722
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 5.1
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +1
Query: 166 VFDHWQVLPGDPCEPQSKPYNV 231
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 6.8
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +3
Query: 27 SWSRFRRVPGGRY-TYVHCEGL 89
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 6.8
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +2
Query: 86 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 190
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 27.1 bits (57), Expect = 9.0
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Frame = +2
Query: 89 TYLSMSRSVLLPICVPTPADRP---SRSAYSTIGRSSLETRANLRA 217
+Y S SRSV P +P RP SRS YS++ RS +++ R+
Sbjct: 212 SYSSKSRSVS-PARSISPRSRPLSRSRSLYSSVSRSQSRSKSRSRS 256
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,800,901
Number of Sequences: 28952
Number of extensions: 240061
Number of successful extensions: 563
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 563
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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