BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0454 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 30 0.37 SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr ... 29 0.85 SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 29 0.85 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 28 1.5 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 27 2.0 SPBC16D10.01c ||SPBC418.03c|conserved fungal protein|Schizosacch... 27 2.6 SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 27 2.6 SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 27 3.4 SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc... 27 3.4 SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces pomb... 26 6.0 SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 25 7.9 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 7.9 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 25 7.9 >SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 29.9 bits (64), Expect = 0.37 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +1 Query: 277 YRAINPAQKVPALEIDGVTLVESMAIIQYI 366 Y ++P K P + DGVT +ES AI++++ Sbjct: 42 YTKLSPLGKSPIVVDDGVTYIESAAILEHL 71 >SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 28.7 bits (61), Expect = 0.85 Identities = 28/108 (25%), Positives = 42/108 (38%), Gaps = 2/108 (1%) Frame = -2 Query: 545 SSFSPVFCEAKEFSSVPKCFFRPKFCNGCIPDTIVSHISLIRARCSAVSGMSFGSGLVSS 366 +S P FCE ++ P F P + + +L+ SAVS + + S Sbjct: 356 TSVEPTFCETNASVALSTVTSTPVFSTDSSPLSSRTRENLLSLIPSAVSSPTKANTNKSH 415 Query: 365 MYCIIAIDSTK--VTPSISRAGTFCAGLMALYSSVSCLPFLQYPPDVL 228 +TK TPS + A T + YS + +PF P D L Sbjct: 416 QRSFSIPKATKDSQTPSENSAATLKQAAIDAYSQIPVIPFF-LPSDKL 462 >SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharomyces pombe|chr 3|||Manual Length = 230 Score = 28.7 bits (61), Expect = 0.85 Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 3/44 (6%) Frame = +1 Query: 250 KNGKQLTEEYRAINPAQKVPAL---EIDGVTLVESMAIIQYIED 372 + G+Q +E+ A+NP +VP L + + T+ ES AI+ Y+ D Sbjct: 37 QKGEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLAD 80 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -2 Query: 344 DSTKVTPSISRAGTFCAGLMALYSSVSCLPFLQYPPD 234 +S K+ +IS G+F A L YSS S + + P D Sbjct: 31 ESLKLKSNISHTGSFFANLSQSYSSTSIIHSVSSPVD 67 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 27.5 bits (58), Expect = 2.0 Identities = 25/83 (30%), Positives = 38/83 (45%), Gaps = 3/83 (3%) Frame = -2 Query: 620 ISQRNLISDAVSS*CFR*QIIERLQSSFSPVFCEAKEFSSVPKCFFRPK-FCNGCIPDTI 444 +S +N+ S+ F ++E+ S +C K +S+ KC K FCN + Sbjct: 15 VSSKNMTSENGLEHQFEELLVEKQYSEEHCAYCHIKNPNSILKCLHCNKWFCN-VRGKSG 73 Query: 443 VSHI--SLIRARCSAVSGMSFGS 381 SHI L+RAR V+ S S Sbjct: 74 ASHIISHLVRARHKQVALHSHSS 96 >SPBC16D10.01c ||SPBC418.03c|conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 336 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +3 Query: 561 DLLAKTSGAYCIGDQITLADICLVP 635 DL K GAYC ++ + D+C+ P Sbjct: 117 DLKKKIQGAYCSIAELFMTDLCMQP 141 >SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 27.1 bits (57), Expect = 2.6 Identities = 16/64 (25%), Positives = 29/64 (45%) Frame = +1 Query: 349 AIIQYIEDTRPEPKLMPDTALQRARMREICETIVSGIQPLQNFGLKKHLGTEENSLASQN 528 ++ +Y + + L+P + IC I SG+ L N G+ H T E +++ Sbjct: 460 SVFEYKDPSTKLSALIPKYFFSELNIASICYEISSGLAFLHNSGIAHHNLTTECIYLTKS 519 Query: 529 TGLK 540 + LK Sbjct: 520 SCLK 523 >SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharomyces pombe|chr 3|||Manual Length = 229 Score = 26.6 bits (56), Expect = 3.4 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 3/54 (5%) Frame = +1 Query: 220 TFRRTSGGYCKNGKQLTEEYRAINPAQKVPAL---EIDGVTLVESMAIIQYIED 372 T+ + KN +Q + E+ A+NP +VP L + T+ ES AI+ Y+ D Sbjct: 28 TYETRYVNFSKN-EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLAD 80 >SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 26.6 bits (56), Expect = 3.4 Identities = 16/90 (17%), Positives = 40/90 (44%), Gaps = 1/90 (1%) Frame = +1 Query: 250 KNGKQLTEEYRAINPAQKVPALEIDGVTLVESMAIIQYIEDTRP-EPKLMPDTALQRARM 426 K+ ++ ++YR+I + + G ++ S + I + PKL+P T Sbjct: 634 KDNSKIRKDYRSIQSITSRNKVSLKGASVDISSKDKEIISQKKQLSPKLIPSTPYDLPSS 693 Query: 427 REICETIVSGIQPLQNFGLKKHLGTEENSL 516 +I + ++ + P +++ L ++ S+ Sbjct: 694 SQISSSALAQLPPSMQSDIQQQLRLQKRSI 723 >SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 505 Score = 25.8 bits (54), Expect = 6.0 Identities = 16/63 (25%), Positives = 27/63 (42%) Frame = -3 Query: 637 CGTRQISANVI*SPMQ*APDVFANKSLSVCSPLSVQYFVKLRNSPPFPNVSSDRNFVMVV 458 CG R +V+ P + ++ +C P++ Q V + +PP P S F+ V Sbjct: 332 CGQRGGYLDVVNIPEPAKDQILKLATIDICPPVAGQLLVDMLVNPPKPGDPSYDLFIKEV 391 Query: 457 YRI 449 I Sbjct: 392 DEI 394 >SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/32 (37%), Positives = 16/32 (50%) Frame = +3 Query: 345 YGDNTVHRRYET*TETHARHSTTTRPYERNMR 440 +G + H Y T H RHSTT +R+ R Sbjct: 149 HGSHRRHEPYRTHLSRHHRHSTTNYHSKRDDR 180 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/34 (26%), Positives = 16/34 (47%) Frame = -2 Query: 344 DSTKVTPSISRAGTFCAGLMALYSSVSCLPFLQY 243 D T + + +FC + YSS+ C ++Y Sbjct: 726 DGTSIAHWLQGLASFCGRVFRRYSSLDCTSIVEY 759 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -3 Query: 262 VYHFYNIHRTFFERYA 215 VYHF I R+ +E YA Sbjct: 685 VYHFIGIQRSLYENYA 700 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,958,447 Number of Sequences: 5004 Number of extensions: 61941 Number of successful extensions: 174 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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