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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0424
         (544 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g45000.1 68415.m05603 expressed protein contains Pfam profile...    31   0.66 
At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom...    30   0.87 
At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1) ide...    28   3.5  
At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative st...    28   3.5  

>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
           PF05064 Nsp1-like C-terminal region
          Length = 739

 Score = 30.7 bits (66), Expect = 0.66
 Identities = 19/53 (35%), Positives = 26/53 (49%)
 Frame = +2

Query: 2   TAATGMTVTPASTSVVKGQSTTLTVAFXPEGVTASSFRAVSADKTKATVSVXG 160
           T  T  + TPA+TS     +   T+AF   GVT+S+     A  + AT S  G
Sbjct: 397 TTTTTSSSTPAATSAPASSAPASTMAFPSFGVTSSATNTTPA-SSAATFSTTG 448


>At5g16780.1 68418.m01965 SART-1 family protein contains Pfam
           domain, PF03343: SART-1 family
          Length = 820

 Score = 30.3 bits (65), Expect = 0.87
 Identities = 16/39 (41%), Positives = 18/39 (46%)
 Frame = +1

Query: 4   SGNRHDRDACQHLGGERAEHHADRGLXAGGRNRXELSCG 120
           S   H+ D   H   ER EH  +RGL  GG N    S G
Sbjct: 132 SKKSHEDDDETHKAAERYEHSDNRGLNEGGDNVDAASSG 170


>At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1)
           identical to 2-isopropylmalate synthase (IMS1)
           [Arabidopsis thaliana] GI:12330687;  identical to cDNA
           2-isopropylmalate synthase (IMS1) GI:12330686
          Length = 631

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = -1

Query: 259 GEVISATAANSPLPDTTGMLTLPAE 185
           GEVI A A    +PDT G +TLP+E
Sbjct: 247 GEVIKAGATTLNIPDTVG-ITLPSE 270


>At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative
           strong similarity to 2-isopropylmalate synthase (IMS1)
           [Arabidopsis thaliana] GI:12330687; contains Pfam
           profile PF00682: HMGL-like
          Length = 631

 Score = 28.3 bits (60), Expect = 3.5
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = -1

Query: 259 GEVISATAANSPLPDTTGMLTLPAE 185
           GEVI A A    +PDT G +TLP+E
Sbjct: 249 GEVIKAGATTLNIPDTVG-ITLPSE 272


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,048,854
Number of Sequences: 28952
Number of extensions: 166781
Number of successful extensions: 403
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 403
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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