BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0424 (544 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 31 0.66 At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom... 30 0.87 At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1) ide... 28 3.5 At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative st... 28 3.5 >At2g45000.1 68415.m05603 expressed protein contains Pfam profile: PF05064 Nsp1-like C-terminal region Length = 739 Score = 30.7 bits (66), Expect = 0.66 Identities = 19/53 (35%), Positives = 26/53 (49%) Frame = +2 Query: 2 TAATGMTVTPASTSVVKGQSTTLTVAFXPEGVTASSFRAVSADKTKATVSVXG 160 T T + TPA+TS + T+AF GVT+S+ A + AT S G Sbjct: 397 TTTTTSSSTPAATSAPASSAPASTMAFPSFGVTSSATNTTPA-SSAATFSTTG 448 >At5g16780.1 68418.m01965 SART-1 family protein contains Pfam domain, PF03343: SART-1 family Length = 820 Score = 30.3 bits (65), Expect = 0.87 Identities = 16/39 (41%), Positives = 18/39 (46%) Frame = +1 Query: 4 SGNRHDRDACQHLGGERAEHHADRGLXAGGRNRXELSCG 120 S H+ D H ER EH +RGL GG N S G Sbjct: 132 SKKSHEDDDETHKAAERYEHSDNRGLNEGGDNVDAASSG 170 >At1g74040.1 68414.m08574 2-isopropylmalate synthase 1 (IMS1) identical to 2-isopropylmalate synthase (IMS1) [Arabidopsis thaliana] GI:12330687; identical to cDNA 2-isopropylmalate synthase (IMS1) GI:12330686 Length = 631 Score = 28.3 bits (60), Expect = 3.5 Identities = 14/25 (56%), Positives = 17/25 (68%) Frame = -1 Query: 259 GEVISATAANSPLPDTTGMLTLPAE 185 GEVI A A +PDT G +TLP+E Sbjct: 247 GEVIKAGATTLNIPDTVG-ITLPSE 270 >At1g18500.1 68414.m02309 2-isopropylmalate synthase, putative strong similarity to 2-isopropylmalate synthase (IMS1) [Arabidopsis thaliana] GI:12330687; contains Pfam profile PF00682: HMGL-like Length = 631 Score = 28.3 bits (60), Expect = 3.5 Identities = 14/25 (56%), Positives = 17/25 (68%) Frame = -1 Query: 259 GEVISATAANSPLPDTTGMLTLPAE 185 GEVI A A +PDT G +TLP+E Sbjct: 249 GEVIKAGATTLNIPDTVG-ITLPSE 272 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,048,854 Number of Sequences: 28952 Number of extensions: 166781 Number of successful extensions: 403 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 394 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 403 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1013649368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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