BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0409 (700 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.06c |||methyltransferase |Schizosaccharomyces pombe|chr... 27 2.0 SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.4 SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 26 6.0 >SPAC23C4.06c |||methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 327 Score = 27.5 bits (58), Expect = 2.0 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = -1 Query: 121 LFF*TSHV*SKFSRKKKNTNSVVDNIKIIIDLIFYVLSRA 2 L+F SH + + +N NS+VD+I I D + V S A Sbjct: 78 LYFPASHQQIQLTLHLENQNSIVDSIHINADTVLPVWSEA 117 >SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 26.6 bits (56), Expect = 3.4 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 4/46 (8%) Frame = +2 Query: 473 KYFNFLSG*TLYFASSLASRSSDCEIST----*PDFNYILYCRRIF 598 K F+F + +Y A SL SR S EIS+ PDF +L R++ Sbjct: 45 KAFSFKNQDQIYGAGSLKSRFSTHEISSEKNDSPDFTLVLPSNRLY 90 >SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosaccharomyces pombe|chr 1|||Manual Length = 180 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/28 (35%), Positives = 19/28 (67%) Frame = -3 Query: 185 FKKTFTVQYTVYV*SSFKTRFSFLLNKS 102 F+++FT++ ++Y SFK + +L KS Sbjct: 79 FRQSFTIEGSIYEVGSFKVAIANVLQKS 106 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,717,874 Number of Sequences: 5004 Number of extensions: 51557 Number of successful extensions: 92 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 91 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 92 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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