Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0360
(701 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ... 43 2e-04
At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain... 42 4e-04
At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar ... 38 0.009
At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar ... 36 0.026
At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar ... 34 0.079
At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar ... 33 0.18
At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar ... 33 0.24
At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar ... 32 0.32
At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar ... 31 0.74
At4g10360.1 68417.m01701 expressed protein 30 1.3
At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar ... 30 1.3
At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar ... 30 1.7
At2g03040.1 68415.m00257 transmembrane protein-related low simil... 29 2.3
At1g75850.1 68414.m08810 vacuolar protein sorting-associated pro... 29 2.3
At3g02430.1 68416.m00230 hypothetical protein contains Pfam prof... 29 3.9
At2g37500.1 68415.m04599 arginine biosynthesis protein ArgJ fami... 28 5.2
At4g39230.1 68417.m05553 isoflavone reductase, putative similar ... 28 6.9
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 28 6.9
At1g06660.1 68414.m00706 expressed protein 28 6.9
>At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24
family; contains non-consensus TG acceptor splice site
at exon 3
Length = 217
Score = 42.7 bits (96), Expect = 2e-04
Identities = 20/61 (32%), Positives = 34/61 (55%)
Frame = +1
Query: 484 QRTCYYIKTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYSQEV 663
+R+ Y I+ + Y+++R+ R INE TN+RV + V +V++I QV Y +
Sbjct: 151 RRSTEYASAIRANILYLRIREAYMREINEKTNTRVNQLGLMSLGVAIVVSISQVLYLKRY 210
Query: 664 F 666
F
Sbjct: 211 F 211
>At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains
weak similarity to transmembrane protein (GI:1212965)
[Homo sapiens]
Length = 214
Score = 41.9 bits (94), Expect = 4e-04
Identities = 18/53 (33%), Positives = 28/53 (52%)
Frame = +2
Query: 263 ILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIG 421
+ P GN++ + S + F AP +G Y +CF N S TP+ V F + +G
Sbjct: 73 VTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYS--TPETVSFYIHVG 123
Score = 40.7 bits (91), Expect = 0.001
Identities = 18/55 (32%), Positives = 34/55 (61%)
Frame = +1
Query: 502 IKTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYSQEVF 666
++++ +Q+Y++ RD HR NEST RV+ +++ E L + QV Y +++F
Sbjct: 151 LESVVAEQKYLKARDTRHRHTNESTRKRVIFYTVGEYIFLAAASGLQVLYIRKLF 205
>At1g21900.1 68414.m02741 emp24/gp25L/p24 family protein similar to
SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Mesocricetus
auratus}; contains Pfam profile PF01105: emp24/gp25L/p24
family
Length = 216
Score = 37.5 bits (83), Expect = 0.009
Identities = 17/55 (30%), Positives = 32/55 (58%)
Frame = +1
Query: 502 IKTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYSQEVF 666
+ +I+ + Y++ R+ R ++E+TNSRV +SI V +V+ Q+ Y + F
Sbjct: 156 VLSIRENLNYIKDREAEMREVSETTNSRVAWFSIMSLGVCVVVVGSQILYLKRYF 210
Score = 30.7 bits (66), Expect = 0.98
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 263 ILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCF 364
+ P GN +H E + G + F+ AG Y CF
Sbjct: 73 VTSPYGNNLHHQENVTHGQFAFTTQEAGNYLACF 106
>At3g07680.1 68416.m00921 emp24/gp25L/p24 family protein similar to
SP|Q15363 Cop-coated vesicle membrane protein p24
precursor (p24A) {Homo sapiens}; contains Pfam profile
PF01105: emp24/gp25L/p24 family
Length = 208
Score = 35.9 bits (79), Expect = 0.026
Identities = 21/83 (25%), Positives = 37/83 (44%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTK 436
++I GP G IH + S + F G Y +CF+NK S + + F++++G
Sbjct: 65 LVIHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNK--SPYHETIDFDVQLGHFAYY 122
Query: 437 SGNENEADHNKLEDMIKELATTL 505
+ + L + I +L L
Sbjct: 123 DQHAKDEHFTPLMEQISKLEEAL 145
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/52 (25%), Positives = 29/52 (55%)
Frame = +1
Query: 511 IKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVMTIGQVYYSQEVF 666
I+ +Q +++ + +NE+ + R V ++FE+ L+ + QVY + +F
Sbjct: 148 IQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLRRLF 199
>At1g69460.1 68414.m07981 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105:
emp24/gp25L/p24 family
Length = 214
Score = 34.3 bits (75), Expect = 0.079
Identities = 31/92 (33%), Positives = 45/92 (48%), Gaps = 3/92 (3%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKV---VMFNLEIGEP 427
V + GN H AE+ SG + FSA AG Y CF+ P+V + F + G
Sbjct: 70 VKVTSNSGNNYHHAEQVDSGQFAFSAVEAGDYMACFT--AVDHKPEVSLSIDFEWKTG-V 126
Query: 428 QTKSGNENEADHNKLEDMIKELATTLRLSNTI 523
Q+KS N A +++E M E+ + L N+I
Sbjct: 127 QSKSW-ANVAKKSQVEVMEFEVKSLLDTVNSI 157
>At3g29070.1 68416.m03636 emp24/gp25L/p24 family protein similar to
SP|Q28735 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Oryctolagus
cuniculus}; contains Pfam profile: PF01105
emp24/gp25L/p24 family
Length = 204
Score = 33.1 bits (72), Expect = 0.18
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +2
Query: 248 RYYVIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCF 364
+ +V + P G H AE SG + F+A G Y CF
Sbjct: 57 KLFVTVSSPKGKSHHHAENVESGKFVFTAEETGDYMTCF 95
>At1g57620.1 68414.m06539 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 32.7 bits (71), Expect = 0.24
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLE 415
V + P G V+H E ++G + F+ +G Y CF S K N++
Sbjct: 67 VKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINID 119
>At1g09580.1 68414.m01075 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 217
Score = 32.3 bits (70), Expect = 0.32
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLE 415
V + P GN +H E + G + F+ +G Y CF S K V N++
Sbjct: 72 VKVTSPYGNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNVSINID 124
>At1g14010.1 68414.m01654 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 212
Score = 31.1 bits (67), Expect = 0.74
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFS 367
V + P G H+++ SG ++F A G Y CFS
Sbjct: 68 VRVTSPQGTAYHESDGVESGQFSFVAVETGDYISCFS 104
>At4g10360.1 68417.m01701 expressed protein
Length = 266
Score = 30.3 bits (65), Expect = 1.3
Identities = 22/87 (25%), Positives = 41/87 (47%)
Frame = -2
Query: 595 TSPHVNLYFHLLNDGYDHAPAYTLDRV**S*CSSKFFDHVLKFIMVSFIFITRFSLWFSD 416
T+P VNL ++L N G + AYTL+ + + F ++ +++ F L F
Sbjct: 164 TTPFVNLRWYLDNSGQKGSKAYTLNGI------ALFLGWLVARVLLFIFFFVHMYLHFHQ 217
Query: 415 FKIEHYHLGCHG*HFITEAISILPCSW 335
K + + LG + I A++++ W
Sbjct: 218 VK-QVFPLGFYSLLTIPPALAVMNLLW 243
>At2g03290.1 68415.m00284 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 170
Score = 30.3 bits (65), Expect = 1.3
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFS 367
V ++ P G +H+A++ +G ++F+A G Y C +
Sbjct: 26 VKVMPPQGKNLHEADKVEAGQFSFTAYENGSYVACIT 62
>At1g26690.1 68414.m03251 emp24/gp25L/p24 family protein similar to
SP|P49755 Transmembrane protein Tmp21 precursor (21 kDa
Transmembrane trafficking protein) {Homo sapiens};
contains Pfam profile PF01105: emp24/gp25L/p24 family
Length = 214
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 278 GNVIHKAERESSGIYTFSAPTAGKYTYCFS 367
GN H AE SG + F+A +G Y C++
Sbjct: 77 GNTYHHAEDVESGQFAFTAVESGDYMACYT 106
>At2g03040.1 68415.m00257 transmembrane protein-related low
similarity to SP|Q28735|TM21_RABIT Transmembrane protein
Tmp21 precursor (21 kDa Transmembrane trafficking
protein) (Integral membrane protein p23) {Oryctolagus
cuniculus}
Length = 166
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESSGIYTFSAPTAGKYTYCFS 367
V ++ P G +H+A+ +G ++F+A G Y C +
Sbjct: 69 VKVMPPQGKNLHEADNVEAGQFSFTAYENGSYVACIT 105
>At1g75850.1 68414.m08810 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 838
Score = 29.5 bits (63), Expect = 2.3
Identities = 17/57 (29%), Positives = 24/57 (42%)
Frame = +2
Query: 296 AERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADHN 466
A R SSG T KY Y F +TP + +E+ + +S N N H+
Sbjct: 752 ATRGSSGPVTLFVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHS 808
>At3g02430.1 68416.m00230 hypothetical protein contains Pfam profile
PF05078: Protein of unknown function (DUF679)
Length = 219
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +3
Query: 78 KY-LRISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADTK 209
KY +R W+ TL LV A + D + +CFY + EA+TK
Sbjct: 139 KYRMRFVDWIHATLSVLVFGAVALR---DKYITDCFYPSPEAETK 180
>At2g37500.1 68415.m04599 arginine biosynthesis protein ArgJ family
contains Pfam profile: PF01960 ArgJ family
Length = 468
Score = 28.3 bits (60), Expect = 5.2
Identities = 17/71 (23%), Positives = 34/71 (47%)
Frame = +2
Query: 299 ERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADHNKLED 478
E ++G++T + A YC +S T + V+ N G+ +G +A + + D
Sbjct: 98 EAVAAGVFTTNVVAAAPVVYCKKVLETSKTARAVLIN--AGQANAATG---DAGYQDMLD 152
Query: 479 MIKELATTLRL 511
+ +AT L++
Sbjct: 153 CVGSIATLLKV 163
>At4g39230.1 68417.m05553 isoflavone reductase, putative similar to
allergenic isoflavone reductase-like protein Bet v
6.0102 [Betula pendula][GI:10764491]; contains Pfam
profile PF02716: Isoflavone reductase
Length = 308
Score = 27.9 bits (59), Expect = 6.9
Identities = 28/101 (27%), Positives = 45/101 (44%), Gaps = 3/101 (2%)
Frame = +2
Query: 257 VIILGPDGNVIHKAERESS-GIYTFSAPTAGKYTYCFSNKMSSMTPKV--VMFNLEIGEP 427
VI+LG DGN +E G YT +A + NK+ + P + FN +
Sbjct: 176 VIVLG-DGNPKAVFNKEEDIGTYTINAVDDPRTL----NKILYIRPPMNTYSFNDLVSLW 230
Query: 428 QTKSGNENEADHNKLEDMIKELATTLRLSNTIKSICRCVIV 550
+ K G E + E ++K++ + N + S+C CV V
Sbjct: 231 ENKIGKTLERIYVPEEQLLKQIIESSPPLNVMLSLCHCVFV 271
>At1g79280.1 68414.m09242 expressed protein weak similarity to
Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
Length = 2111
Score = 27.9 bits (59), Expect = 6.9
Identities = 18/89 (20%), Positives = 37/89 (41%)
Frame = +2
Query: 284 VIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNENEADH 463
V KA ES + C +M + + + + E + N + AD+
Sbjct: 1319 VAQKARMESENFENLLKTKQTELDLCMK-EMEKLRMETDLHKKRVDELRETYRNIDIADY 1377
Query: 464 NKLEDMIKELATTLRLSNTIKSICRCVIV 550
N+L+D +++L L+ + C+ V++
Sbjct: 1378 NRLKDEVRQLEEKLKAKDAHAEDCKKVLL 1406
>At1g06660.1 68414.m00706 expressed protein
Length = 481
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 87 RISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADT 206
RI V ++ N++ NA Y V D+H + E +EA+T
Sbjct: 290 RIRSQFVHSVSNIMENASLYKVYKDSHEGLDYEEQIEAET 329
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,772,956
Number of Sequences: 28952
Number of extensions: 297698
Number of successful extensions: 722
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 722
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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