BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0335 (613 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 26 4.9 SPAC869.11 ||SPAC922.08c|amino acid permease, unknown 6|Schizosa... 26 4.9 SPAC821.12 |orb6||serine/threonine protein kinase Orb6|Schizosac... 25 6.5 SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 25 8.6 SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 25 8.6 >SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 942 Score = 25.8 bits (54), Expect = 4.9 Identities = 15/32 (46%), Positives = 17/32 (53%) Frame = +3 Query: 267 DMNFHVNRTSKINVIHNQNSSDTAKLNSLFHN 362 DMNF N T+KIN N S TA FH+ Sbjct: 565 DMNFDFNDTNKIN-----NPSSTANQTRHFHS 591 >SPAC869.11 ||SPAC922.08c|amino acid permease, unknown 6|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 25.8 bits (54), Expect = 4.9 Identities = 15/29 (51%), Positives = 17/29 (58%), Gaps = 2/29 (6%) Frame = -1 Query: 142 NIFTLYYIHLCFIIYRLLFNI--HTLNEL 62 N FT I LC II+RL F H+L EL Sbjct: 444 NFFTWGSICLCHIIFRLAFKKQGHSLKEL 472 >SPAC821.12 |orb6||serine/threonine protein kinase Orb6|Schizosaccharomyces pombe|chr 1|||Manual Length = 469 Score = 25.4 bits (53), Expect = 6.5 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +1 Query: 412 KNKQVLCSNRLATQRLCCPKQKRSMRHYAITKIICKGNFNYIK 540 KN+Q+ S +Q L + + S+ ++ K+I KG F ++ Sbjct: 66 KNRQLRASGEKESQFLRFRRTRLSLEDFSTIKVIGKGAFGEVR 108 >SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 950 Score = 25.0 bits (52), Expect = 8.6 Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Frame = +3 Query: 222 YDVASETTNLKLNSPDMNFHVNRTSKINVIHNQNSSDTAKL--NSLFHNTRTEL 377 YD ++TNLKL S ++ I ++ SSDT L L+ + R +L Sbjct: 840 YDAIEQSTNLKLKSISQEDTIDLMRNQTFILSRISSDTILLVPKKLYPSKRKKL 893 >SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1811 Score = 25.0 bits (52), Expect = 8.6 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = +3 Query: 216 HGYDVASETTNLKLNSPDMNFHVNRTSKINVIHNQN 323 H YD+ S++ KL S + +H+ RT +N++ + N Sbjct: 395 HEYDLKSQSMRSKLMSLHLIYHILRT-YMNILSDIN 429 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,412,957 Number of Sequences: 5004 Number of extensions: 46904 Number of successful extensions: 130 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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