BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0312 (642 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 132 5e-32 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 98 1e-21 SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3... 32 0.081 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 28 1.3 SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 5.3 SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc... 26 5.3 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 7.0 SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 25 9.3 SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 25 9.3 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 132 bits (318), Expect = 5e-32 Identities = 57/83 (68%), Positives = 67/83 (80%) Frame = +1 Query: 244 SCTRACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGGYKSRESVPKLVDEYL 423 S ACHKGWG S++IGVAAAG+EISTRPFQLVTGR W+G AFGG K R +P LV EYL Sbjct: 282 SALEACHKGWGQSIVIGVAAAGQEISTRPFQLVTGRVWRGCAFGGVKGRSQLPDLVKEYL 341 Query: 424 EKKLPLDEFVTHNVPLKEINEAF 492 + KL +D+++TH PLKEINEAF Sbjct: 342 DHKLEIDKYITHRRPLKEINEAF 364 Score = 83.8 bits (198), Expect = 2e-17 Identities = 35/63 (55%), Positives = 45/63 (71%) Frame = +2 Query: 68 RIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTMRA 247 RI G+D+NP+K A FG +F+NP D PIQ VL+ TDGGL++TF+C GNV MR+ Sbjct: 223 RIFGIDVNPEKKNWAMSFGATDFINPNDLQSPIQDVLIHETDGGLDWTFDCTGNVHVMRS 282 Query: 248 ALE 256 ALE Sbjct: 283 ALE 285 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 97.9 bits (233), Expect = 1e-21 Identities = 42/78 (53%), Positives = 55/78 (70%) Frame = +1 Query: 259 CHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGGYKSRESVPKLVDEYLEKKLP 438 CHKGWG +IGVAAAG+ + RPF +VTGR G+AFGG K R +P VDEY++ Sbjct: 283 CHKGWGKLCVIGVAAAGKTLDFRPFLVVTGRQVLGSAFGGVKGRSELPNFVDEYMQGHFK 342 Query: 439 LDEFVTHNVPLKEINEAF 492 +DE++T+ PLK IN+AF Sbjct: 343 VDEYITNEEPLKNINKAF 360 Score = 65.7 bits (153), Expect = 5e-12 Identities = 29/64 (45%), Positives = 43/64 (67%) Frame = +2 Query: 65 NRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTMR 244 +RII +DIN DK AKKFG +F++ K + Q ++D+TDGG++Y F+C GNV M+ Sbjct: 219 SRIIAIDINADKEVYAKKFGATDFIDSSKV-KDLVQYVIDVTDGGVDYAFDCTGNVTVMQ 277 Query: 245 AALE 256 L+ Sbjct: 278 QELQ 281 >SPCC70.08c |||methyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 260 Score = 31.9 bits (69), Expect = 0.081 Identities = 10/26 (38%), Positives = 19/26 (73%) Frame = +2 Query: 68 RIIGVDINPDKFEVAKKFGVNEFVNP 145 R++G+D +PD + A++ G+N +V P Sbjct: 57 RVVGIDASPDMIKAARELGLNAYVIP 82 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = -2 Query: 368 AVPFQVRPVTSWNGRVLISSPAAATPMIT 282 +VP+ PVTS N + S+P +TP+ T Sbjct: 501 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 529 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = -2 Query: 368 AVPFQVRPVTSWNGRVLISSPAAATPMIT 282 +VP+ PVTS N + S+P +TP+ T Sbjct: 560 SVPYTSTPVTSSNYTISSSTPVTSTPVTT 588 >SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 575 Score = 25.8 bits (54), Expect = 5.3 Identities = 11/35 (31%), Positives = 17/35 (48%) Frame = +1 Query: 274 GVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGG 378 G V+IG + G E++ + +T T G GG Sbjct: 110 GALVVIGTSLGGHELTNGGKEFITSATSLGALLGG 144 >SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 489 Score = 25.8 bits (54), Expect = 5.3 Identities = 11/31 (35%), Positives = 14/31 (45%), Gaps = 3/31 (9%) Frame = +3 Query: 294 CSCCW---RRDQHSSIPTCYRSHLEGNSFWR 377 C CW + D + P C R + E N WR Sbjct: 42 CRFCWHHIKEDLNGRCPACRRLYTEENVQWR 72 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 25.4 bits (53), Expect = 7.0 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = -3 Query: 430 SSPSTHLQALVH-FLYFCNLQKLFPSRCD 347 SSPS+ L+ F Y CN+ KL+ + D Sbjct: 914 SSPSSDLEVKQQAFTYVCNISKLYVYKSD 942 >SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 25.0 bits (52), Expect = 9.3 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = +3 Query: 555 IYTIFVHLLNLFSSAFTTS 611 IYT+F HLLN++ S Sbjct: 1720 IYTLFEHLLNIYKETINDS 1738 >SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pombe|chr 3|||Manual Length = 983 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/31 (32%), Positives = 18/31 (58%) Frame = -3 Query: 184 IHQYLLNWFIIILRVDKFIDSKFLSYLKLVR 92 +H L +W +I+ + DS LSY+++ R Sbjct: 185 LHSSLSSWMMILESSKEIKDSTLLSYMQISR 215 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,442,271 Number of Sequences: 5004 Number of extensions: 49232 Number of successful extensions: 146 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 138 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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