Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0266
         (693 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase sm...   136   1e-32
At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p...    29   3.9  
At2g21500.2 68415.m02559 expressed protein                             29   3.9  
At2g21500.1 68415.m02558 expressed protein                             29   3.9  
At5g42490.1 68418.m05172 kinesin motor family protein contains P...    28   6.8  
At4g23520.1 68417.m03390 cysteine proteinase, putative contains ...    28   6.8  

>At2g21790.1 68415.m02590 ribonucleoside-diphosphate reductase small
           chain, putative / ribonucleotide reductase, putative
           similar to ribonucleotide reductase GI:4151068 from
           [Nicotiana tabacum]
          Length = 816

 Score =  136 bits (330), Expect = 1e-32
 Identities = 61/84 (72%), Positives = 69/84 (82%)
 Frame = +1

Query: 1   PGLADCWGKEFESLYETYEKENRFVXQVRAQVLWKAIIEAQVETGTPYMLFKDSCNRKSN 180
           PGLADCWG EFE+LY  YE+E +    V+AQ LW  I+ +QVETGTPYMLFKDSCNRKSN
Sbjct: 357 PGLADCWGAEFETLYTKYEREGKAKKVVQAQQLWYEILTSQVETGTPYMLFKDSCNRKSN 416

Query: 181 QKNLGTIKCSNLCTEIVEYTSDDE 252
           Q+NLGTIK SNLCTEI+EYTS  E
Sbjct: 417 QQNLGTIKSSNLCTEIIEYTSPTE 440



 Score =  121 bits (292), Expect = 4e-28
 Identities = 72/144 (50%), Positives = 91/144 (63%), Gaps = 19/144 (13%)
 Frame = +3

Query: 252 SAVCNLASIALNMFVDD-------------------NKTYNFKKLKEVTKTITENLNKII 374
           +AVCNLASIAL  FV +                   N+ ++F+KL EVT T+T NLNKII
Sbjct: 441 TAVCNLASIALPRFVREKGVPLDSHPPKLAGSLDSKNRYFDFEKLAEVTATVTVNLNKII 500

Query: 375 DVNYYPVPEAKKSNLRHRPIGIGVQGLADAFILMRLPYESPEAIN*INKYLKLFTMGALE 554
           DVNYYPV  AK SN+RHRPIGIGVQGLADAFIL+ +P++SPEA        +     AL+
Sbjct: 501 DVNYYPVETAKTSNMRHRPIGIGVQGLADAFILLGMPFDSPEAQQLNKDIFETIYYHALK 560

Query: 555 ASCELAAKVWECMIHTTDAPASRG 626
           AS ELAA++     +   +P S+G
Sbjct: 561 ASTELAARLGPYETY-AGSPVSKG 583


>At2g26300.1 68415.m03156 guanine nucleotide binding protein
           (G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
           identical to SP|P18064 Guanine nucleotide-binding
           protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
           thaliana}
          Length = 383

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +3

Query: 315 NFKKLKEVTKTITENLNKIIDVNYYPVPE 401
           N  ++ + TK + ENL ++ D+NY P  E
Sbjct: 156 NELQVPDCTKYLMENLKRLSDINYIPTKE 184


>At2g21500.2 68415.m02559 expressed protein
          Length = 421

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/67 (25%), Positives = 25/67 (37%)
 Frame = +3

Query: 300 DNKTYNFKKLKEVTKTITENLNKIIDVNYYPVPEAKKSNLRHRPIGIGVQGLADAFILMR 479
           D        +  V++ IT+  N   D  YYP P     +L  +P    V  L+    L  
Sbjct: 97  DQSFSRIASIDSVSEQITQKTNDSTDPTYYPSPAKLSLSLASQPSSFSVSPLSSQSHLPM 156

Query: 480 LPYESPE 500
            P  S +
Sbjct: 157 PPASSSQ 163


>At2g21500.1 68415.m02558 expressed protein
          Length = 421

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/67 (25%), Positives = 25/67 (37%)
 Frame = +3

Query: 300 DNKTYNFKKLKEVTKTITENLNKIIDVNYYPVPEAKKSNLRHRPIGIGVQGLADAFILMR 479
           D        +  V++ IT+  N   D  YYP P     +L  +P    V  L+    L  
Sbjct: 97  DQSFSRIASIDSVSEQITQKTNDSTDPTYYPSPAKLSLSLASQPSSFSVSPLSSQSHLPM 156

Query: 480 LPYESPE 500
            P  S +
Sbjct: 157 PPASSSQ 163


>At5g42490.1 68418.m05172 kinesin motor family protein contains Pfam
           domain, PF00225: Kinesin motor domain
          Length = 1087

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 11/41 (26%), Positives = 22/41 (53%)
 Frame = +3

Query: 261 CNLASIALNMFVDDNKTYNFKKLKEVTKTITENLNKIIDVN 383
           CN +S+  N+  DDN    +  LKE  K + + +  ++ ++
Sbjct: 660 CNESSVCKNIGTDDNDNDTYMALKEKVKEMQKKIEYLMSMH 700


>At4g23520.1 68417.m03390 cysteine proteinase, putative contains
           similarity to cysteine proteinase (thiol protease) RD21A
           GI:435619, SP:P43297 from [Arabidopsis thaliana]
          Length = 356

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 18/70 (25%), Positives = 33/70 (47%), Gaps = 5/70 (7%)
 Frame = +3

Query: 285 NMFVDDNKTYNFKKLKEVTKTITENLNKIIDVNYYP-VPEAKKSNLR----HRPIGIGVQ 449
           N  +D  K Y ++  +          NK+I ++ Y  VP   + +L+    H+P+ +GV 
Sbjct: 211 NNGLDSEKDYPYQGTQGSCNRKQSTSNKVITIDSYEDVPANDEISLQKAVAHQPVSVGVD 270

Query: 450 GLADAFILMR 479
             +  F+L R
Sbjct: 271 KKSQEFMLYR 280


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,514,215
Number of Sequences: 28952
Number of extensions: 247492
Number of successful extensions: 568
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 557
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 567
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)