BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0259 (518 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g41460.1 68418.m05035 fringe-related protein strong similarit... 31 0.35 At4g23490.1 68417.m03384 fringe-related protein + weak similari... 28 4.3 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 31.5 bits (68), Expect = 0.35 Identities = 19/41 (46%), Positives = 21/41 (51%) Frame = -1 Query: 425 NQDYYSGKL**FKQHLQNTYFDLSTAWGGMLETGHQIKYEL 303 NQ YY G L + HLQN YF A+GG G I Y L Sbjct: 231 NQMYYIGSL--SESHLQNIYFSYGMAYGG---GGFAISYPL 266 >At4g23490.1 68417.m03384 fringe-related protein + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 526 Score = 27.9 bits (59), Expect = 4.3 Identities = 17/42 (40%), Positives = 21/42 (50%) Frame = -1 Query: 422 QDYYSGKL**FKQHLQNTYFDLSTAWGGMLETGHQIKYELVQ 297 Q YY G L + HLQN +F A+GG G I Y L + Sbjct: 233 QMYYIGSL--SESHLQNIFFSYGMAYGG---GGFAISYPLAK 269 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,110,710 Number of Sequences: 28952 Number of extensions: 194348 Number of successful extensions: 350 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 348 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 350 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 947539968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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