BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0228 (445 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1685.10 |rps27||40S ribosomal protein S27|Schizosaccharomyce... 105 2e-24 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 6.9 SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|... 25 6.9 >SPBC1685.10 |rps27||40S ribosomal protein S27|Schizosaccharomyces pombe|chr 2|||Manual Length = 83 Score = 105 bits (253), Expect = 2e-24 Identities = 43/63 (68%), Positives = 51/63 (80%) Frame = +2 Query: 77 LSGLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVCAGCSTILCQPTGGRARLTEGCSFK 256 L LV P S+FMDVKCPGC+ ITTVFSHAQ VV+C C+++LCQPTGG+ARL EGCSF+ Sbjct: 21 LKQLVQGPRSFFMDVKCPGCFNITTVFSHAQTVVICGSCASVLCQPTGGKARLMEGCSFR 80 Query: 257 ENN 265 N Sbjct: 81 RKN 83 Score = 35.5 bits (78), Expect = 0.004 Identities = 16/23 (69%), Positives = 19/23 (82%) Frame = +1 Query: 16 MPLAIDLLHPSPASERRKHKLKR 84 M LA+DLL+PS SE RKHKLK+ Sbjct: 1 MVLAVDLLNPSHESEMRKHKLKQ 23 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 24.6 bits (51), Expect = 6.9 Identities = 12/18 (66%), Positives = 13/18 (72%) Frame = +2 Query: 41 ILPLRRRGGNISLSGLVP 94 ILPL RRG I SGL+P Sbjct: 512 ILPLLRRGIGIHHSGLLP 529 >SPBC3H7.10 |||elongator homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 24.6 bits (51), Expect = 6.9 Identities = 9/29 (31%), Positives = 17/29 (58%) Frame = -1 Query: 178 HHSLCVTKNCCNLVTARALNIHEIRVRMW 92 +H+L ++ C L ++ L+ H I +R W Sbjct: 37 YHALKAKESTCFLTFSKTLDEHAISMRKW 65 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,659,597 Number of Sequences: 5004 Number of extensions: 29802 Number of successful extensions: 62 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 61 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 62 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 162176800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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