BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0224 (635 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe... 100 2e-22 SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyce... 44 2e-05 SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 29 0.74 SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 27 2.3 SPAC15A10.06 |||CPA1 sodium ion/proton antiporter|Schizosaccharo... 26 4.0 SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 26 5.2 SPAC869.04 |||formamidase-like protein|Schizosaccharomyces pombe... 26 5.2 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 25 6.9 SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|... 25 6.9 SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 9.1 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 9.1 >SPCC830.08c |||Golgi membrane protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 182 Score = 100 bits (239), Expect = 2e-22 Identities = 43/113 (38%), Positives = 71/113 (62%) Frame = +3 Query: 177 FLRLGRVHRFIFSVRFRS*INMQLDRFVYPAYMSMKALESPQKDDDTKWLTYWVVYACFS 356 FL ++ + + + L F PA+ S+ A+E+ K DDT+WLTY++V + + Sbjct: 39 FLTAAGIYALFLFLNWGGFLLTNLLAFAMPAFFSINAIETTNKADDTQWLTYYLVTSFLN 98 Query: 357 IVEYFSDFIVGWFPLYWLLKCIFVIWCYLPTEYNGSLVIYYRIIRPYYQKHMV 515 ++EY+S I+ + P+YWLLK IF+IW LP ++NG+ +IY +IRPY H++ Sbjct: 99 VIEYWSQLILYYVPVYWLLKAIFLIWLALP-KFNGATIIYRHLIRPYITPHVI 150 Score = 31.9 bits (69), Expect = 0.080 Identities = 16/66 (24%), Positives = 32/66 (48%), Gaps = 2/66 (3%) Frame = +1 Query: 76 YKDNIEQSLNDKSKPWTKYFEL--AEQKVGVNRLYIFLGLVAFTGLYLVFGFGAELICNS 249 ++ ++Q++ D + +L E+ GV++LY+FL L+L +G L+ N Sbjct: 3 FQVRVKQNMQDLDNRLAAFPQLNSLEKNFGVSKLYVFLTAAGIYALFLFLNWGGFLLTNL 62 Query: 250 IGSCTP 267 + P Sbjct: 63 LAFAMP 68 >SPBC30D10.09c |||HVA22/TB2/DP1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 44.0 bits (99), Expect = 2e-05 Identities = 19/69 (27%), Positives = 30/69 (43%) Frame = +3 Query: 303 KDDDTKWLTYWVVYACFSIVEYFSDFIVGWFPLYWLLKCIFVIWCYLPTEYNGSLVIYYR 482 +++ + + YW VY C + E + W P Y K +F +W P G+ IY Sbjct: 97 EEERRRLMAYWCVYGCVTAAESILGRFLSWVPFYSTSKIVFWLWLLNP-RTQGAAFIYAS 155 Query: 483 IIRPYYQKH 509 I P+ H Sbjct: 156 YISPFLSDH 164 >SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubiquinone oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 28.7 bits (61), Expect = 0.74 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = -1 Query: 230 APKPNTKYKPVNATKPKKMYNLFTPTFCSASS 135 A K +KYKP+N KP + + PT S S+ Sbjct: 510 ATKSASKYKPINYPKPDNVLSFDIPTSVSRSA 541 >SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1 complex subunit Tpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1039 Score = 27.1 bits (57), Expect = 2.3 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = +2 Query: 473 LLPYHSSLLPEAHGRI 520 +L YH L+PEAHG I Sbjct: 483 VLQYHLGLIPEAHGNI 498 >SPAC15A10.06 |||CPA1 sodium ion/proton antiporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 26.2 bits (55), Expect = 4.0 Identities = 13/41 (31%), Positives = 25/41 (60%) Frame = +1 Query: 97 SLNDKSKPWTKYFELAEQKVGVNRLYIFLGLVAFTGLYLVF 219 +++ K+K TKY ++ N ++I+LG+ FT + LV+ Sbjct: 307 NMSYKAKLSTKYVFRVLAQLSENFIFIYLGMSLFTQVDLVY 347 >SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +1 Query: 31 G*PLKDRTMASKLQEYKDNIEQSLNDKSKPW 123 G P + + L EYK E+S+ND S W Sbjct: 20 GDPTVPKPNIASLDEYKRMYEESINDPSTFW 50 >SPAC869.04 |||formamidase-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 410 Score = 25.8 bits (54), Expect = 5.2 Identities = 10/24 (41%), Positives = 13/24 (54%) Frame = +3 Query: 348 CFSIVEYFSDFIVGWFPLYWLLKC 419 C ++EYF F + LY LL C Sbjct: 337 CLRVIEYFRRFGYNDYQLYLLLSC 360 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +1 Query: 61 SKLQEYKDNIEQSLNDKSKPWTKYFE 138 +++ E D I++ LNDK K W F+ Sbjct: 53 NEMLEVMDIIDRRLNDKGKNWRHVFK 78 >SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 329 Score = 25.4 bits (53), Expect = 6.9 Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 2/41 (4%) Frame = +3 Query: 330 YWVVYACFSIVEY--FSDFIVGWFPLYWLLKCIFVIWCYLP 446 Y Y FSI + FSDF++ YW+ + + W Y P Sbjct: 146 YGYFYLFFSIALFLLFSDFLI-----YWIHRALHHRWLYAP 181 >SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 25.0 bits (52), Expect = 9.1 Identities = 11/28 (39%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = -2 Query: 142 LVQNISS-MACFCRSDFVLCCLYTLVIW 62 L+++IS+ +ACF F+LC + + V+W Sbjct: 225 LIKSISTYIACFAMLIFLLCNVGSHVVW 252 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 25.0 bits (52), Expect = 9.1 Identities = 15/56 (26%), Positives = 30/56 (53%) Frame = +2 Query: 377 FHRRLVSIVLAVKVHLRDLVLPAD*IQRVPRDLLPYHSSLLPEAHGRIDDMGQHRE 544 + RRL+SI+ +++ H+R+ + P + R+ R+ P S P + D +R+ Sbjct: 439 YERRLLSIMESIRPHIRN-IDPFGGLSRLGREEYPRRLSNPPPSQKTNQDAWTNRK 493 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,902,492 Number of Sequences: 5004 Number of extensions: 65609 Number of successful extensions: 193 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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