BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0221 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 111 7e-26 SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 111 7e-26 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 3.4 SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch... 27 3.4 SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 26 5.9 SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 5.9 SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 25 7.9 >SPBC336.10c |tif512||translation initiation factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 111 bits (268), Expect = 7e-26 Identities = 49/66 (74%), Positives = 57/66 (86%) Frame = +3 Query: 57 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 236 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 237 VGIDIF 254 V +DIF Sbjct: 61 VALDIF 66 Score = 105 bits (253), Expect = 5e-24 Identities = 44/87 (50%), Positives = 67/87 (77%) Frame = +2 Query: 254 HGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQL 433 +G+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG ++ Sbjct: 67 NGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEI 125 Query: 434 RTDFDSGKELLCTVLKSCGEECVIASK 514 F+ GK+L+ TV+ + GEE +A + Sbjct: 126 EEGFEEGKDLIITVVSAMGEEIALACR 152 >SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 111 bits (268), Expect = 7e-26 Identities = 49/66 (74%), Positives = 57/66 (86%) Frame = +3 Query: 57 MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 236 M + E FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+ Sbjct: 1 MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60 Query: 237 VGIDIF 254 V +DIF Sbjct: 61 VALDIF 66 Score = 106 bits (255), Expect = 3e-24 Identities = 45/92 (48%), Positives = 68/92 (73%) Frame = +2 Query: 254 HGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQL 433 +G+KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM +G ++D+++P+G+LG ++ Sbjct: 67 NGRKYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEI 125 Query: 434 RTDFDSGKELLCTVLKSCGEECVIASKQTQLS 529 FD G++L+ TV+ + GEE +A + S Sbjct: 126 EEGFDEGRDLIITVVSAMGEETALACRDAPSS 157 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 26.6 bits (56), Expect = 3.4 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = +2 Query: 293 NMDVPHVKREDYQLTDISDDGYL 361 NM +P+++ E Q+ D + D YL Sbjct: 968 NMSIPYIRGEYVQMIDANQDNYL 990 >SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 204 Score = 26.6 bits (56), Expect = 3.4 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 149 FRYAEGSSMQDC*NVHIQNRKARPR*SSLGWD 244 + + E +Q C N+ +N +A R SS GWD Sbjct: 41 YHHLEKGLLQQCFNLVKKNMEALYRQSSFGWD 72 >SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 340 Score = 25.8 bits (54), Expect = 5.9 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +2 Query: 470 TVLKSCGEECVIASKQTQLSTNKPTQHL*G 559 TV K+ EECV+AS +T L T PT + G Sbjct: 306 TVAKT--EECVLASIETFLDTGIPTNPVNG 333 >SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 3971 Score = 25.8 bits (54), Expect = 5.9 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%) Frame = -1 Query: 504 MTHSSPQDFSTVHNNSLPLSKS--VRNCVPRSPSGILRSS 391 + S+P STV N+S P++ S + +P + S +L SS Sbjct: 1254 LNSSTPITSSTVVNSSTPITSSTALNTSIPITSSSVLNSS 1293 Score = 25.4 bits (53), Expect = 7.9 Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 2/48 (4%) Frame = -1 Query: 504 MTHSSPQDFSTVHNNSLPLSKS--VRNCVPRSPSGILRSSRRSPLSAI 367 + S+P STV N+S P++ S + P + S +L SS S+I Sbjct: 3138 LNSSTPITSSTVVNSSTPITSSTTLNTSTPITSSSVLNSSTAITSSSI 3185 >SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 875 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/17 (58%), Positives = 14/17 (82%) Frame = +2 Query: 311 VKREDYQLTDISDDGYL 361 +KREDY+LT ++ GYL Sbjct: 91 IKREDYELTLCANCGYL 107 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,982,986 Number of Sequences: 5004 Number of extensions: 62868 Number of successful extensions: 220 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 218 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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