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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0221
         (693 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g13950.1 68414.m01639 eukaryotic translation initiation facto...   104   6e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto...   101   3e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto...   101   5e-22
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ...    29   2.9  
At5g03140.1 68418.m00262 lectin protein kinase family protein co...    29   3.9  
At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,...    29   3.9  
At3g51290.1 68416.m05614 proline-rich family protein                   28   5.1  
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont...    28   6.8  
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-...    27   8.9  
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila...    27   8.9  
At2g28550.1 68415.m03468 AP2 domain-containing transcription fac...    27   8.9  
At1g56345.1 68414.m06477 pseudouridine synthase family protein l...    27   8.9  
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein...    27   8.9  
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF...    27   8.9  

>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
           5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Arabidopsis thaliana}
          Length = 158

 Score =  104 bits (249), Expect = 6e-23
 Identities = 43/72 (59%), Positives = 56/72 (77%)
 Frame = +3

Query: 69  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
           E+ HFE+ D+GAS T+P Q   +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 249 IFMVKSMKISVP 284
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75



 Score =  104 bits (249), Expect = 6e-23
 Identities = 47/86 (54%), Positives = 65/86 (75%), Gaps = 1/86 (1%)
 Frame = +2

Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 436
           KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG  ++DLK+P+ D L  Q++
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127

Query: 437 TDFDSGKELLCTVLKSCGEECVIASK 514
           + FD GK+L+ +V+ + GEE + A K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153


>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similarity to
           eukaryotic initiation factor 5A (2) (Nicotiana
           plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Lycopersicon esculentum}
          Length = 158

 Score =  101 bits (243), Expect = 3e-22
 Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
 Frame = +2

Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
           KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG  ++DLK+P D  L TQL+
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127

Query: 437 TDFDSGKELLCTVLKSCGEECVIASKQ 517
             F+ GK+++ +V+ + GEE + A K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154



 Score =  100 bits (240), Expect = 8e-22
 Identities = 42/72 (58%), Positives = 55/72 (76%)
 Frame = +3

Query: 69  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
           ++ HFE+ D+GAS T+P Q   +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 249 IFMVKSMKISVP 284
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75


>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similariy to
           SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
           (eIF-5A 1) {Lycopersicon esculentum}
          Length = 159

 Score =  101 bits (242), Expect = 5e-22
 Identities = 45/87 (51%), Positives = 64/87 (73%), Gaps = 1/87 (1%)
 Frame = +2

Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
           KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G  ++DLK+P D  L  Q+R
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127

Query: 437 TDFDSGKELLCTVLKSCGEECVIASKQ 517
             FD GK+++ +V+ S GEE + A K+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154



 Score = 96.3 bits (229), Expect = 2e-20
 Identities = 40/72 (55%), Positives = 52/72 (72%)
 Frame = +3

Query: 69  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
           ++ HFE  +SGAS T+P     +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 249 IFMVKSMKISVP 284
           IF  K ++  VP
Sbjct: 64  IFTAKKLEDIVP 75


>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 591

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 15/34 (44%), Positives = 16/34 (47%)
 Frame = +2

Query: 398 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 499
           L IPDGD  T L      G   +C  L SCG  C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445


>At5g03140.1 68418.m00262 lectin protein kinase family protein
           contains Pfam domains, PF00138: Legume lectins alpha
           domain, PF00139: Legume lectins beta domain and PF00069:
           Protein kinase domain
          Length = 711

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = -3

Query: 169 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 62
           +P  +T+P +R     +   A+ PE P++K  SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666


>At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,
           putative strong similarity to SP|P54151 Peptide
           methionine sulfoxide reductase (EC 1.8.4.6) {Brassica
           napus}; contains Pfam profile PF01625: Peptide
           methionine sulfoxide reductase
          Length = 258

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
 Frame = +2

Query: 251 LHGKKYEDICPST--HNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLG 424
           +H   YED+C  T  HN     V R  Y   + S +  L +  +  D    L    GD+G
Sbjct: 126 VHNPSYEDVCTGTTGHN----EVVRVQYDPKECSFESLLDVFWNRHD-PTTLNRQGGDVG 180

Query: 425 TQLRT 439
           TQ R+
Sbjct: 181 TQYRS 185


>At3g51290.1 68416.m05614 proline-rich family protein 
          Length = 602

 Score = 28.3 bits (60), Expect = 5.1
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 46  KTQQWVTSKTHTLRPETPGPQPPSP 120
           +T  W T+ T ++ P  P P PP P
Sbjct: 91  ETTTWTTTTTSSVLPPPPPPPPPPP 115


>At2g27680.1 68415.m03354 aldo/keto reductase family protein
           contains Pfam profile PF00248: oxidoreductase, aldo/keto
           reductase family
          Length = 384

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 26/95 (27%), Positives = 44/95 (46%)
 Frame = +2

Query: 269 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 448
           ++I  S   MDV  +    +   D ++DGYL  +    DL+E     +G + T   T+FD
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201

Query: 449 SGKELLCTVLKSCGEECVIASKQTQLSTNKPTQHL 553
           +  E L  +L++ G   V    Q  +   +P Q +
Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233


>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
           RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
           contains Pfam profiles PF04408: Helicase associated
           domain (HA2), PF00271: Helicase conserved C-terminal
           domain
          Length = 1226

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
 Frame = +2

Query: 311 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 460
           V+R+  +++      Y  L ADN    +   +  G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400


>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
            SP|P35207 Antiviral protein SKI2 {Saccharomyces
            cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
            box helicase, PF00271: Helicase conserved C-terminal
            domain
          Length = 1347

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
 Frame = +2

Query: 341  ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 475
            +SD+  L + A  G  D+ +++   D DL  Q++     + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191


>At2g28550.1 68415.m03468 AP2 domain-containing transcription factor
           RAP2.7 (RAP2.7) nearly identical to AP2 domain
           transcription factor RAP2.7 (GI:2281639) [Arabidopsis
           thaliana]
          Length = 449

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 9/15 (60%), Positives = 12/15 (80%)
 Frame = +1

Query: 70  KTHTLRPETPGPQPP 114
           +T  +RP+ PGPQPP
Sbjct: 402 QTSAVRPQPPGPQPP 416


>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
           similarity to SP|P23851 Ribosomal large subunit
           pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
           synthase) (Uracil hydrolyase) {Escherichia coli};
           contains Pfam profile PF00849: RNA pseudouridylate
           synthase
          Length = 322

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
 Frame = -3

Query: 478 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVRY 302
           +HGA +  AA++V   L     +RD E   + V+V S + +   + D++ ++V A   R 
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIV-AEGER- 233

Query: 301 VHVVCGGTD 275
             + CGG D
Sbjct: 234 -ELSCGGDD 241


>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
           contains INTERPRO domain, IPR001878: Zn-finger CCHC type
          Length = 353

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 15/45 (33%), Positives = 18/45 (40%), Gaps = 3/45 (6%)
 Frame = +1

Query: 70  KTHTLRPETPGPQPPS---PCNVRPCVKMVSLC*RVVHARLLKCP 195
           KT T       P PP    PCN  PC  + S   +  + R   CP
Sbjct: 22  KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66


>At1g35830.1 68414.m04452 VQ motif-containing protein contains
           PF05678: VQ motif
          Length = 302

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = +1

Query: 22  SFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSP 120
           ++L  V       + ++TH L P+ P P PP P
Sbjct: 60  TYLNFVNNLISDDILNQTHLLPPQPPPPPPPPP 92


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,994,191
Number of Sequences: 28952
Number of extensions: 352553
Number of successful extensions: 1005
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 947
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1002
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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