BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0154 (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC11B10.10c |pht1||histone H2A variant|Schizosaccharomyces pom... 124 2e-29 SPCC622.08c |hta1||histone H2A alpha |Schizosaccharomyces pombe|... 97 3e-21 SPAC19G12.06c |hta2||histone H2A beta|Schizosaccharomyces pombe|... 97 3e-21 SPAC1786.02 |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 30 0.28 SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosa... 30 0.36 SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 28 1.1 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 1.9 >SPBC11B10.10c |pht1||histone H2A variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 171 Score = 124 bits (298), Expect = 2e-29 Identities = 60/73 (82%), Positives = 67/73 (91%) Frame = +1 Query: 37 GLQFPVGRIHRHLKNRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITP 216 GLQFPVGR+ R LK +T ++ RVGA +AVYSAA+LEYLTAEVLELAGNA+KDLKVKRITP Sbjct: 63 GLQFPVGRVRRFLKAKTQNNMRVGAKSAVYSAAVLEYLTAEVLELAGNAAKDLKVKRITP 122 Query: 217 RHLQLAIRGDEEL 255 RHLQLAIRGDEEL Sbjct: 123 RHLQLAIRGDEEL 135 Score = 40.7 bits (91), Expect = 2e-04 Identities = 16/23 (69%), Positives = 22/23 (95%) Frame = +3 Query: 252 IDSLIKATIAGGGVIPHIHKSLI 320 +D+LI+ATIAGGGV+PHI+K L+ Sbjct: 135 LDTLIRATIAGGGVLPHINKQLL 157 >SPCC622.08c |hta1||histone H2A alpha |Schizosaccharomyces pombe|chr 3|||Manual Length = 132 Score = 96.7 bits (230), Expect = 3e-21 Identities = 50/84 (59%), Positives = 61/84 (72%) Frame = +1 Query: 37 GLQFPVGRIHRHLKNRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITP 216 GL FPVGR+HR L+ + RVGA A VY AA+LEYL AE+LELAGNA++D K RI P Sbjct: 24 GLAFPVGRVHRLLRKGNYAQ-RVGAGAPVYLAAVLEYLAAEILELAGNAARDNKKTRIIP 82 Query: 217 RHLQLAIRGDEELTAS*KQLSLAE 288 RHLQLAIR DEEL +++A+ Sbjct: 83 RHLQLAIRNDEELNKLLGHVTIAQ 106 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/21 (52%), Positives = 15/21 (71%) Frame = +3 Query: 273 TIAGGGVIPHIHKSLIGKKGG 335 TIA GGV+P+I+ L+ K G Sbjct: 103 TIAQGGVVPNINAHLLPKTSG 123 >SPAC19G12.06c |hta2||histone H2A beta|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 96.7 bits (230), Expect = 3e-21 Identities = 50/84 (59%), Positives = 61/84 (72%) Frame = +1 Query: 37 GLQFPVGRIHRHLKNRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITP 216 GL FPVGR+HR L+ + RVGA A VY AA+LEYL AE+LELAGNA++D K RI P Sbjct: 24 GLAFPVGRVHRLLRKGNYAQ-RVGAGAPVYLAAVLEYLAAEILELAGNAARDNKKTRIIP 82 Query: 217 RHLQLAIRGDEELTAS*KQLSLAE 288 RHLQLAIR DEEL +++A+ Sbjct: 83 RHLQLAIRNDEELNKLLGHVTIAQ 106 Score = 31.5 bits (68), Expect = 0.12 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = +3 Query: 273 TIAGGGVIPHIHKSLIGKKGGPGAP 347 TIA GGV+P+I+ L+ K+ G G P Sbjct: 103 TIAQGGVVPNINAHLLPKQSGKGKP 127 >SPAC1786.02 |||phospholipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 30.3 bits (65), Expect = 0.28 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +3 Query: 132 RYFGISYSRGFGVGGKCV*RFKSEAYYSSALTTCY 236 +Y G +YS G V GKCV +F + + +T Y Sbjct: 311 QYLGTNYSNGTAVDGKCVTQFDNVGFLVGTSSTRY 345 >SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 29.9 bits (64), Expect = 0.36 Identities = 17/70 (24%), Positives = 34/70 (48%) Frame = +1 Query: 43 QFPVGRIHRHLKNRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRH 222 +FPV RI + + G+V V + LE +++ + ++ + KR+T H Sbjct: 23 RFPVARIKK-IMQADQDVGKVAQVTPVIMSKALELFMQSIIQESCKQTRLHQAKRVTVSH 81 Query: 223 LQLAIRGDEE 252 L+ A++ E+ Sbjct: 82 LKHAVQSVEQ 91 >SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 28.3 bits (60), Expect = 1.1 Identities = 15/59 (25%), Positives = 33/59 (55%) Frame = -3 Query: 582 SLILIQSYFKSPYSLHISITYSYILVRTNSSSHTTVHFTNIILIENYVNQSGR*FVQVC 406 +L + ++ K S ++ + YI+ R + +SH + F++I+ I V + G F+++C Sbjct: 119 TLAINNNFIKGRRSQYVVASCLYIVCRISKTSHMLIDFSDILQIN--VFKLGSTFLKLC 175 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 27.5 bits (58), Expect = 1.9 Identities = 17/60 (28%), Positives = 26/60 (43%), Gaps = 1/60 (1%) Frame = -1 Query: 245 SPLIASCKCRGVIRFTFKS-LDAFPANSKTSAVRYSKIAAE*TAAVAPTRPWLVVLFLRC 69 SPL CKC G IR+ + L + +SK + K E T + + P + + C Sbjct: 19 SPLFHPCKCTGSIRYVHQECLVEWLGHSKKTHCELCKAKFEFTKVYSESMPRTIPFTILC 78 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,790,826 Number of Sequences: 5004 Number of extensions: 56650 Number of successful extensions: 155 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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