Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= prgv0056
         (678 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3 |Sc...    75   1e-14
SPBC1289.07c |rpc40|rpa42|DNA-directed RNA polymerase I and III ...    37   0.003
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc...    28   1.1  
SPBC2F12.02c |mrpl7||mitochondrial ribosomal protein subunit L7|...    28   1.4  
SPAC10F6.07c |mug94||sequence orphan|Schizosaccharomyces pombe|c...    27   1.9  
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    27   3.3  
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit...    25   7.6  

>SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 297

 Score = 74.5 bits (175), Expect = 1e-14
 Identities = 33/74 (44%), Positives = 50/74 (67%), Gaps = 4/74 (5%)
 Frame = +1

Query: 256 AIDWVQLEANSTVLSDEFLAHRIGLIPLISDDVVDK----IRYSRDCMCVDFCSECSVEF 423
           AID V++  N++V+ DEFLAHR+G+IPL S ++ +     + Y+R+C C  +C +CSVE 
Sbjct: 44  AIDLVEINVNTSVMPDEFLAHRLGMIPLDSSNIDEPPPVGLEYTRNCDCDQYCPKCSVEL 103

Query: 424 TLDVKCTDEQTRHV 465
            L+ KCT E T  +
Sbjct: 104 FLNAKCTGEGTMEI 117



 Score = 28.7 bits (61), Expect = 0.82
 Identities = 13/24 (54%), Positives = 15/24 (62%)
 Frame = +2

Query: 575 KLRKGQELKLRAYAKKGFGERACK 646
           KLRK QE+ LR  AKKG  +   K
Sbjct: 147 KLRKEQEISLRCIAKKGIAKEHAK 170



 Score = 28.7 bits (61), Expect = 0.82
 Identities = 11/17 (64%), Positives = 13/17 (76%)
 Frame = +3

Query: 618 KKVLGKEHAKWNPTGWV 668
           KK + KEHAKW+PT  V
Sbjct: 161 KKGIAKEHAKWSPTSAV 177


>SPBC1289.07c |rpc40|rpa42|DNA-directed RNA polymerase I and III
           subunit Rpc40 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 348

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 19/63 (30%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
 Frame = +1

Query: 256 AIDWVQLEANSTVLSDEFLAHRIGLIPLISD-DVVDKIRYSRDCMCVDFCSECSVEFTLD 432
           A ++V +  N++++ DE L+HRIGL+P+ +D D+    ++             +V F+L+
Sbjct: 85  AFEFVYIINNTSIIQDEVLSHRIGLVPISADPDMFKWFQHPLPGQEATHTDYDTVVFSLN 144

Query: 433 VKC 441
            KC
Sbjct: 145 KKC 147



 Score = 26.6 bits (56), Expect = 3.3
 Identities = 15/36 (41%), Positives = 18/36 (50%)
 Frame = +2

Query: 539 PIMVKQMKY*FIKLRKGQELKLRAYAKKGFGERACK 646
           PI V        KLR GQE+ L A+A  G G+   K
Sbjct: 190 PIRVVNPDIVVAKLRPGQEIDLEAHAILGIGQDHAK 225


>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
           Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 554

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = -1

Query: 642 HALSPKPFLAYARSFNS*PLRNLMNQYFIC 553
           H  + K + A  +SFN+ PL  +MN+ F+C
Sbjct: 197 HKYNIKVYEACLQSFNALPLAAIMNKQFLC 226


>SPBC2F12.02c |mrpl7||mitochondrial ribosomal protein subunit
           L7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 287

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = -3

Query: 571 ESIFHLLHHNRPGLHLYVVM*RDNSRIARFQISS 470
           E+++ +  H+ PG ++ +     ++R+ARF +SS
Sbjct: 240 EAVYEMYPHSLPGFNVNITTNSKDTRLARFFVSS 273


>SPAC10F6.07c |mug94||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 188

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 14/47 (29%), Positives = 24/47 (51%)
 Frame = -2

Query: 413 LHSEQKSTHMQSREYLILSTTSSDMSGMRPMRCAKNSSLKTVELASN 273
           L S QKST   ++   +L+ + +  +G+   RC  +S L  + L  N
Sbjct: 74  LSSNQKSTSKSNQMIKLLNNSLAHNNGLPSQRCNHDSKLVYMSLVQN 120


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 7/26 (26%), Positives = 18/26 (69%)
 Frame = +3

Query: 120 KMPYANQPSVHITELSDENVKFVVED 197
           + P A+ P+++  + + EN++ ++ED
Sbjct: 83  RQPIADVPAIYFVQPTQENIELIIED 108


>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
           Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 546

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 9/36 (25%), Positives = 21/36 (58%)
 Frame = +1

Query: 544 YGEADEILIH*VTQGSGIEASGICQKRFWGKSMQNG 651
           YGEA E++   +++ +G++ + +  K +     +NG
Sbjct: 441 YGEAFEVVPRTISENAGLDPTDVISKLYAAHHKENG 476


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,806,876
Number of Sequences: 5004
Number of extensions: 57080
Number of successful extensions: 143
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)