BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0050
(695 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g02940.1 68417.m00401 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 3.9
At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 29 3.9
At4g38890.1 68417.m05508 dihydrouridine synthase family protein ... 28 6.8
At3g45830.1 68416.m04960 expressed protein 28 6.8
At1g59850.1 68414.m06741 expressed protein 27 9.0
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 27 9.0
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 27 9.0
>At4g02940.1 68417.m00401 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to A. thaliana hypothetical
protein T13L16.2, GenBank accession number 2708738;
contains Pfam domain PF03171 2OG-Fe(II) oxygenase
superfamily
Length = 569
Score = 28.7 bits (61), Expect = 3.9
Identities = 26/85 (30%), Positives = 41/85 (48%), Gaps = 4/85 (4%)
Frame = +1
Query: 4 HESLDSSSEEISPTKESITADESRGHFYNFGSHYEGLTLPKKKNRNPQFA-LEDLDVLDL 180
HE D+ S EI P K ++ +GH N +GL L ++ + + + L D V +L
Sbjct: 209 HEDCDARSCEIKPIKGFQAKEQVKGHTVNV---VKGLKLYEELLKEDEISKLLDF-VAEL 264
Query: 181 TEIGEN---IGDQFGIFGGRKRQNK 246
E G N G+ F +F + + NK
Sbjct: 265 REAGINGKLAGESFILFNKQIKGNK 289
>At1g09020.1 68414.m01006 protein kinase, putative similar to
protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
profile PF00571: CBS domain
Length = 487
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = +1
Query: 112 LTLPKKKNRNPQFALEDLDVLDLTEIGENIGDQFGIFGGRKRQNKLKPTNIVTV 273
+T K Q L+D+ V ++G++ +GIF G++ L+ ++V V
Sbjct: 396 ITALAKDKAYAQIHLDDMTVHQALQLGQDASPPYGIFNGQRCHMCLRSDSLVKV 449
>At4g38890.1 68417.m05508 dihydrouridine synthase family protein
contains Pfam domain, PF01207: Dihydrouridine synthase
(Dus)
Length = 700
Score = 27.9 bits (59), Expect = 6.8
Identities = 17/63 (26%), Positives = 33/63 (52%), Gaps = 4/63 (6%)
Frame = +1
Query: 25 SEEISPTKESITADESRGHFYNFGSHYEGLTLPK--KKNRNP--QFALEDLDVLDLTEIG 192
+EE+ P K++ + D+ + G Y+G+ L + KKN P + +ED D + + E
Sbjct: 270 TEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETD 329
Query: 193 ENI 201
++
Sbjct: 330 SSL 332
>At3g45830.1 68416.m04960 expressed protein
Length = 1298
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +3
Query: 267 DGRDGDEYDEMFDVISLDALTRKSDTGNKNIKKDRRRQNKMMRDLWPE 410
DG GDE E +S +AL++K T + +RR N ++ L+P+
Sbjct: 710 DGNSGDENLEARLFVSCNALSKKRKTRESLMDMERREDNGDLQ-LYPD 756
>At1g59850.1 68414.m06741 expressed protein
Length = 498
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +3
Query: 300 FDVISLDALTRKSDTGNKNIKKDRRRQN 383
F VIS D L +S T N N K++ RQN
Sbjct: 471 FSVISTDLLVSRSITQNGNHKRNACRQN 498
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
Frame = -2
Query: 244 YSASFCHQIYQID----HRYFHRFQLNLKHPNPLVQTVDFCFSFWV 119
YSA F Q + ID HR+ H+F + + P+ ++ F FW+
Sbjct: 97 YSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
Frame = -2
Query: 244 YSASFCHQIYQID----HRYFHRFQLNLKHPNPLVQTVDFCFSFWV 119
YSA F Q + ID HR+ H+F + + P+ ++ F FW+
Sbjct: 97 YSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,713,896
Number of Sequences: 28952
Number of extensions: 237688
Number of successful extensions: 582
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 571
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 582
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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