BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= prgv0028 (674 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein... 82 3e-16 At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein... 79 2e-15 At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein... 79 4e-15 At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein... 64 1e-10 At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 44 1e-04 At5g28320.1 68418.m03438 expressed protein This is likely a pseu... 29 3.8 At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein... 28 6.6 At1g52310.1 68414.m05902 protein kinase family protein / C-type ... 27 8.7 >At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein similar to SP|O00264 Membrane associated progesterone receptor component (mPR) {Homo sapiens}; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 233 Score = 82.2 bits (194), Expect = 3e-16 Identities = 42/98 (42%), Positives = 64/98 (65%), Gaps = 1/98 (1%) Frame = +1 Query: 256 LLAVNGIIFDVTRGKRFYGPGGPYSAFAGKDATRGLATGQVAASENDEYDDVSDLGSDEI 435 L+A+ G I+DV++ + FYGPGGPY+ FAGKDA+R LA +++ + D D+S LG+ E+ Sbjct: 89 LMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALA--KMSFEDQDLTGDISGLGAFEL 146 Query: 436 ASAIEWEEQFRGKYGIVGRLLKL-GERQKITVMMRAKT 546 + +WE +F KY VG + K GE ++ + AKT Sbjct: 147 EALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPSEAKT 184 >At5g52240.1 68418.m06484 cytochrome b5 domain-containing protein similar to SP|P70580 Membrane associated progesterone receptor component 1 {Rattus norvegicus}; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 220 Score = 79.4 bits (187), Expect = 2e-15 Identities = 42/102 (41%), Positives = 63/102 (61%) Frame = +1 Query: 256 LLAVNGIIFDVTRGKRFYGPGGPYSAFAGKDATRGLATGQVAASENDEYDDVSDLGSDEI 435 L+A+ I+DVT+ + FYGPGGPY+ FAGKDA+R LA +++ E D DVS LG E+ Sbjct: 93 LMAIKHQIYDVTQSRMFYGPGGPYALFAGKDASRALA--KMSFEEKDLTWDVSGLGPFEL 150 Query: 436 ASAIEWEEQFRGKYGIVGRLLKLGERQKITVMMRAKTKNLSK 561 + +WE +F KY VG +K+ + T + T+N+ + Sbjct: 151 DALQDWEYKFMSKYAKVG-TVKVAGSEPETASVSEPTENVEQ 191 >At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein similar to SP|P70580 Membrane associated progesterone receptor component 1 {Rattus norvegicus}; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 100 Score = 78.6 bits (185), Expect = 4e-15 Identities = 37/80 (46%), Positives = 52/80 (65%) Frame = +1 Query: 259 LAVNGIIFDVTRGKRFYGPGGPYSAFAGKDATRGLATGQVAASENDEYDDVSDLGSDEIA 438 +A+ G +FDVT GK FYG GG YS FAGKDA+R L G+++ +E D + L EI Sbjct: 22 VAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRAL--GKMSKNEEDVSPSLEGLTEKEIN 79 Query: 439 SAIEWEEQFRGKYGIVGRLL 498 + +WE +F KY +VGR++ Sbjct: 80 TLNDWETKFEAKYPVVGRVV 99 >At4g14965.1 68417.m02300 cytochrome b5 domain-containing protein similar to SP|O15173 Membrane associated progesterone receptor component 2 (Steroid receptor protein DG6) {Homo sapiens}; contains Pfam profile PF00173: Heme/Steroid binding domain Length = 245 Score = 63.7 bits (148), Expect = 1e-10 Identities = 31/81 (38%), Positives = 46/81 (56%) Frame = +1 Query: 256 LLAVNGIIFDVTRGKRFYGPGGPYSAFAGKDATRGLATGQVAASENDEYDDVSDLGSDEI 435 LL + G +FDVT+GK YG GG Y+ FAG+DA+R +G + D + L S E+ Sbjct: 60 LLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG--DGLTDSLQGLSSSEV 117 Query: 436 ASAIEWEEQFRGKYGIVGRLL 498 S ++W + Y VG+L+ Sbjct: 118 KSIVDWRGFYSRTYTPVGKLV 138 >At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1347 Score = 43.6 bits (98), Expect = 1e-04 Identities = 34/93 (36%), Positives = 46/93 (49%), Gaps = 5/93 (5%) Frame = +1 Query: 274 IIFDVTR---GKRFYGP-GGPYSAFAGKDATRGL-ATGQVAASENDEYDDVSDLGSDEIA 438 ++FD R GK F G Y+ AG+ RGL TG V DE D SDL + Sbjct: 750 VVFDALRKFDGKEFRQLLPGEYTQMAGRAGRRGLDKTGTVVVMCRDEVPDESDLRRVIVG 809 Query: 439 SAIEWEEQFRGKYGIVGRLLKLGERQKITVMMR 537 SA E QFR Y ++ LL++ E K+ M++ Sbjct: 810 SATRLESQFRLTYIMILHLLRV-EELKVEDMLK 841 >At5g28320.1 68418.m03438 expressed protein This is likely a pseudogene. Length = 967 Score = 28.7 bits (61), Expect = 3.8 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = -2 Query: 391 FLTQQPALWLSLLWRLYQQTPSMGLPVHKIS 299 FLT++ L LS+L+++Y G P H+I+ Sbjct: 812 FLTEKSLLMLSVLFKVYGAMVDSGFPEHEIA 842 >At4g05360.1 68417.m00818 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 735 Score = 27.9 bits (59), Expect = 6.6 Identities = 22/54 (40%), Positives = 28/54 (51%), Gaps = 1/54 (1%) Frame = +1 Query: 319 GPYSAFAGKDATRGLATGQVAASENDEYDDVSDLG-SDEIASAIEWEEQFRGKY 477 GP SA A +T ATG S+ND+ DD DL SDE E+ E ++ Y Sbjct: 439 GPTSASA-TGSTSASATGPATGSDNDQSDD-DDLSISDE-----EFAENYKALY 485 >At1g52310.1 68414.m05902 protein kinase family protein / C-type lectin domain-containing protein contains protein kinase domain, Pfam:PF00069, PF00059 Lectin C-type domain Length = 552 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 3/59 (5%) Frame = -1 Query: 563 GLLRFLVFALIITVIFWRSPNLRRRPTMPYFPLNCSS---HSMAEAISSEPRSLTSSYS 396 GL+ F FA+I+ ++ ++ RR+ P + SS S S E RS+T ++S Sbjct: 210 GLILFTTFAIILWLLVYKRSKKRRKSRKVSNPASSSSVVPPSWKIFTSEELRSMTKNFS 268 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,257,666 Number of Sequences: 28952 Number of extensions: 250566 Number of successful extensions: 668 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 656 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 664 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1432596384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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