Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= prgv0020
(603 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 100 1e-21
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 98 3e-21
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 98 5e-21
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 97 8e-21
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.59
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 30 1.4
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 29 1.8
At5g38990.1 68418.m04717 protein kinase family protein contains ... 29 3.1
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 3.1
At3g46390.1 68416.m05029 hypothetical protein 28 4.1
At5g02030.1 68418.m00123 homeodomain protein (BELLRINGER) severa... 27 7.2
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 27 7.2
At5g39000.1 68418.m04718 protein kinase family protein contains ... 27 9.6
At2g36270.1 68415.m04452 bZIP transcription factor family protei... 27 9.6
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 99.5 bits (237), Expect = 1e-21
Identities = 45/64 (70%), Positives = 51/64 (79%)
Frame = +2
Query: 317 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINK 496
A K +KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WGASKLVPVGYGI K
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGIKK 182
Query: 497 LQIM 508
L IM
Sbjct: 183 LTIM 186
Score = 34.3 bits (75), Expect = 0.063
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 12 KSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 131
K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 24 KTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 98.3 bits (234), Expect = 3e-21
Identities = 46/80 (57%), Positives = 57/80 (71%)
Frame = +2
Query: 308 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYG 487
+A + K S K KSS+L+D+KPWDDETDMK++E VR+I+MEGL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYG 186
Query: 488 INKLQIMVLSRTTKFSVDLL 547
I KL IM S+D +
Sbjct: 187 IKKLHIMCTIVDDLVSIDTM 206
Score = 40.3 bits (90), Expect = 0.001
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +3
Query: 12 KSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 125
+SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 23 RSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 97.9 bits (233), Expect = 5e-21
Identities = 45/80 (56%), Positives = 58/80 (72%)
Frame = +2
Query: 308 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYG 487
+A + K S K KSS+L+D+KPWDDETDMK++E V++I+MEGL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGASKLVPVGYG 186
Query: 488 INKLQIMVLSRTTKFSVDLL 547
I KLQI+ S+D +
Sbjct: 187 IKKLQILCTIVDDLVSIDTM 206
Score = 39.9 bits (89), Expect = 0.001
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +3
Query: 12 KSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 125
+SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 23 RSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 97.1 bits (231), Expect = 8e-21
Identities = 47/77 (61%), Positives = 56/77 (72%)
Frame = +2
Query: 317 ADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGINK 496
A K +KK KSS+LL+VKPWDDETDMK++E VR+++M GL WGASKLVPVGYGI K
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYGIKK 186
Query: 497 LQIMVLSRTTKFSVDLL 547
L IM+ SVD L
Sbjct: 187 LTIMMTIVDDLVSVDNL 203
Score = 35.9 bits (79), Expect = 0.021
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +3
Query: 12 KSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASY 134
K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 24 KTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.59
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -3
Query: 457 SPEKAFHFNSAYLVFHFLHIGFIIPW 380
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 29.9 bits (64), Expect = 1.4
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 7/41 (17%)
Frame = +3
Query: 24 SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 125
+G TPS ADV VF + + +PHV+RW N I
Sbjct: 92 NGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.5 bits (63), Expect = 1.8
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +3
Query: 12 KSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 152
K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 238 KPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +1
Query: 94 FPTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQ 213
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2
Query: 326 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 457
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At3g46390.1 68416.m05029 hypothetical protein
Length = 69
Score = 28.3 bits (60), Expect = 4.1
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +2
Query: 305 LKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGA--SKLVPV 478
+K Y+ K K+ + +I +D+K +M + E + I M G++W S L+ V
Sbjct: 1 MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60
Query: 479 GYGINKLQI 505
+ K QI
Sbjct: 61 DEALLKQQI 69
>At5g02030.1 68418.m00123 homeodomain protein (BELLRINGER) several
homeodomain proteins;
Length = 575
Score = 27.5 bits (58), Expect = 7.2
Identities = 18/52 (34%), Positives = 22/52 (42%)
Frame = -3
Query: 556 FSGQQINRKLCRPR*HHNLQFIYAIPNRHKFGGSPEKAFHFNSAYLVFHFLH 401
F G +R LC H +A P G PE+A A+L HFLH
Sbjct: 323 FGGSDSSRGLCSAGQRHGFPDHHA-PVWRPHRGLPERAVTVLRAWLFDHFLH 373
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 27.5 bits (58), Expect = 7.2
Identities = 9/36 (25%), Positives = 18/36 (50%)
Frame = +2
Query: 347 IAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLW 454
+ S++L D+K WD+ M+ + ++ G W
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSW 570
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = +1
Query: 97 PTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQQ 216
P YYD I+ + L++N G A + P+++P L P +
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNR 429
>At2g36270.1 68415.m04452 bZIP transcription factor family protein /
ABA-responsive element-binding protein, putative similar
to ABA-responsive element binding protein 1 (AREB1)
GI:9967417 from [Arabidopsis thaliana]; contains a bZIP
transcription factor basic domain signature (PDOC00036)
Length = 442
Score = 27.1 bits (57), Expect = 9.6
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -2
Query: 215 CWGGSRGFSTGGQWAGALRP 156
C+GG GF GGQ G + P
Sbjct: 293 CYGGGVGFGAGGQQMGMVGP 312
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,076,011
Number of Sequences: 28952
Number of extensions: 241927
Number of successful extensions: 664
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 644
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 663
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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