Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0992.Seq
(765 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 128 6e-31
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 113 3e-26
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 57 2e-09
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.14
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.7
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 2.9
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 26 5.1
SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 25 9.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 9.0
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 128 bits (310), Expect = 6e-31
Identities = 67/85 (78%), Positives = 75/85 (88%)
Frame = +3
Query: 255 LVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRL 434
LVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQPRL
Sbjct: 69 LVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQPRL 126
Query: 435 FVGMILILIFAEVLGLYGLIVAIYL 509
FV MILILIFAEVLGLYGLIVA+ L
Sbjct: 127 FVAMILILIFAEVLGLYGLIVALLL 151
Score = 101 bits (242), Expect = 1e-22
Identities = 44/62 (70%), Positives = 55/62 (88%)
Frame = +1
Query: 70 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 249
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 250 YG 255
YG
Sbjct: 67 YG 68
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 113 bits (272), Expect = 3e-26
Identities = 54/85 (63%), Positives = 68/85 (80%)
Frame = +3
Query: 255 LVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRL 434
LV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q R+
Sbjct: 69 LVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQDRI 128
Query: 435 FVGMILILIFAEVLGLYGLIVAIYL 509
FV M+LILIFAEVLGLYGLIV + L
Sbjct: 129 FVSMVLILIFAEVLGLYGLIVGLIL 153
Score = 87.4 bits (207), Expect = 2e-18
Identities = 37/62 (59%), Positives = 48/62 (77%)
Frame = +1
Query: 70 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 249
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 250 YG 255
YG
Sbjct: 67 YG 68
Score = 38.7 bits (86), Expect = 9e-04
Identities = 17/77 (22%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
Frame = +3
Query: 306 PLYKGFIHL-GAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGL 482
P+Y F G ++ FS L AG+ + G A +P + + ++ ++ + ++G+
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 483 YGLIVAIYLTQNKRPEH 533
YGL++++ + + P++
Sbjct: 67 YGLVMSVLIAGDMSPDN 83
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 57.2 bits (132), Expect = 2e-09
Identities = 30/91 (32%), Positives = 48/91 (52%), Gaps = 6/91 (6%)
Frame = +3
Query: 255 LVVAVLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGT 416
L++A++ + + + PA + Y GF G+ VG L G +GI G +
Sbjct: 101 LIIAIVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALAD 160
Query: 417 AQQPRLFVGMILILIFAEVLGLYGLIVAIYL 509
AQ LFV ++++ IF VLGL+GLIV + +
Sbjct: 161 AQDASLFVKVLVVEIFGSVLGLFGLIVGLLI 191
Score = 45.2 bits (102), Expect = 1e-05
Identities = 19/55 (34%), Positives = 33/55 (60%)
Frame = +1
Query: 88 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 252
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIY 99
Score = 40.3 bits (90), Expect = 3e-04
Identities = 20/58 (34%), Positives = 32/58 (55%)
Frame = +3
Query: 330 LGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAI 503
LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y LI+AI
Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAI 105
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.14
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = -3
Query: 487 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 317
P RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.7
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = -3
Query: 418 AVPRTPAS--PTMPMAKPAARPENPTAKPAP 332
A P P S P++PM PAA P P+A AP
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.1 bits (57), Expect = 2.9
Identities = 19/65 (29%), Positives = 28/65 (43%)
Frame = +1
Query: 73 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 252
+YGPF+ A+ FS Y +G G + S+ + LI + I IIA+
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSIKK--LISAASIIYGYTTIIAVL 144
Query: 253 GWSWL 267
W L
Sbjct: 145 LWGIL 149
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 26.2 bits (55), Expect = 5.1
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1
Query: 478 VFTDLSSPSTLHKIN 522
+FTDLSS LHK+N
Sbjct: 822 IFTDLSSTDFLHKVN 836
>SPBC428.08c |clr4||histone H3 methyltransferase
Clr4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 25.4 bits (53), Expect = 9.0
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -2
Query: 662 QELKGAPKPYARWTRYRRRPSLCASSDSDNEHRGS 558
+ L G A W R +RR ++SDSD+ H S
Sbjct: 45 ENLSGCSAVLAEWKRRKRRLK-GSNSDSDSPHHAS 78
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 9.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -3
Query: 418 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 329
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,341,566
Number of Sequences: 5004
Number of extensions: 70696
Number of successful extensions: 218
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 367316502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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