Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0985.Seq
(749 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 147 1e-36
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 91 2e-19
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 48 1e-06
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 44 3e-05
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 28 1.2
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 28 1.2
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 27 2.2
SPCC16C4.16c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.9
SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual 27 2.9
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 26 5.0
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 5.0
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3... 26 5.0
SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 26 6.6
SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch... 25 8.7
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 147 bits (357), Expect = 1e-36
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +3
Query: 3 ITHHNELNMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMA 182
+THHN+L+MDLYMRIAPELY KMLVVGGLDRVYEIGRQFRNEG DLTHNPEFT+ EFY A
Sbjct: 288 VTHHNDLDMDLYMRIAPELYLKMLVVGGLDRVYEIGRQFRNEGADLTHNPEFTSIEFYQA 347
Query: 183 YADYNDLMTITESILSGMVQSIHG 254
YADY DLM TE +LSG+V+ + G
Sbjct: 348 YADYYDLMDTTEELLSGLVKDLTG 371
Score = 118 bits (284), Expect = 9e-28
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +1
Query: 508 EEECINPTFILDHPQIMSPLSKYHRDVPGLTERFEVYVAKKEICNAYTELNDPATQRERF 687
E ECINPTFI+ HPQ+MSPL+KYHR GL ERFE +VA KEICNAYTELND QR RF
Sbjct: 457 ESECINPTFIIGHPQMMSPLAKYHRSDAGLCERFEAFVATKEICNAYTELNDIFDQRARF 516
Query: 688 EEQAKNRAAGDDE 726
EEQA+ +A GDDE
Sbjct: 517 EEQARQKAQGDDE 529
Score = 109 bits (262), Expect = 4e-25
Identities = 48/85 (56%), Positives = 62/85 (72%)
Frame = +2
Query: 254 TYKVKYHPEGPEGKEIEIDFTPPFARVPMMATLERVLNVKLPAPDKLDTPEANDFLNNLC 433
+YKV YHPEGPEG + E+DF+ P+ R+ M+ LE LN K P D+L TPEAN FL +LC
Sbjct: 372 SYKVPYHPEGPEGPKWELDFSRPWRRINMIEYLEEKLNTKFPPGDQLHTPEANAFLRDLC 431
Query: 434 NTHQIECSPPRTTARLLDKLVSVFL 508
H +EC+PP+T +RLLDKLV F+
Sbjct: 432 AKHGVECAPPQTCSRLLDKLVGEFI 456
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 90.6 bits (215), Expect = 2e-19
Identities = 38/76 (50%), Positives = 56/76 (73%)
Frame = +3
Query: 27 MDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYNDLM 206
+ L +R APEL+ K LV+GG++RV+E+G+ FRNEGID THNPEFT+CE Y AY + +
Sbjct: 251 LPLTLRCAPELWLKQLVIGGMNRVFELGKNFRNEGIDATHNPEFTSCEAYCAYLNLEGMK 310
Query: 207 TITESILSGMVQSIHG 254
+TE ++ + +I+G
Sbjct: 311 KLTEELIRFICLTING 326
Score = 73.7 bits (173), Expect = 3e-14
Identities = 36/78 (46%), Positives = 49/78 (62%), Gaps = 6/78 (7%)
Frame = +1
Query: 523 NPTFILDHPQIMSPLSKYHRDVPGLTE-----RFEVYVAKKEICNAYTELNDPATQRERF 687
+P F+++HP++MSPL+K + G E RFE+Y+ EICNAY E NDP Q +F
Sbjct: 407 SPKFVINHPEVMSPLAKSDIKLYGAVEQRISKRFELYIGGYEICNAYEEENDPVAQYHKF 466
Query: 688 EEQAKNR-AAGDDETPQP 738
+ Q +R GDDETP P
Sbjct: 467 QAQKYDRLQLGDDETPAP 484
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 48.4 bits (110), Expect = 1e-06
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 1/78 (1%)
Frame = +3
Query: 48 APELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADY-NDLMTITESI 224
+P+ Y ++L+ G+ Y+I R FR+E + PEFT + M++ D +++M + E +
Sbjct: 214 SPQQYKQILMASGIGNYYQIARCFRDEDLRFDRQPEFTQIDLEMSFVDKPHEIMEVVEDL 273
Query: 225 LSGMVQSIHGPTRSNIIQ 278
L +V G T + Q
Sbjct: 274 LVRLVSFAKGITLAKPFQ 291
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 43.6 bits (98), Expect = 3e-05
Identities = 24/75 (32%), Positives = 43/75 (57%), Gaps = 2/75 (2%)
Frame = +3
Query: 30 DLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGID-LTHNPEFTTCEFYMAYAD-YNDL 203
D ++ +P+LY +ML+ +RV+EIG FR E + H EFT + MA+ + Y+++
Sbjct: 301 DGFLSQSPQLYKQMLIAADRERVFEIGPVFRAEDSNTYRHMTEFTGLDLEMAFNEHYHEV 360
Query: 204 MTITESILSGMVQSI 248
M E + + ++I
Sbjct: 361 MEFIEKLFLYIFKTI 375
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 28.3 bits (60), Expect = 1.2
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +2
Query: 281 GPEGKEIEIDFTPPFARVP---MMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIE 451
G + ++I PF ++P ++ +L VKL D PE ++NNL N+H +E
Sbjct: 390 GNNWRRMDIGIDYPFDQLPDEDIVRFPYAILEVKLQTQFGQDPPE---WVNNLVNSHLVE 446
Query: 452 CSP 460
P
Sbjct: 447 AVP 449
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +3
Query: 6 THHNELNMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCE 170
TH ++ ++ ++A +H + + I R FRNE +D TH EF E
Sbjct: 327 THTTAVSANMLYKLAQNGFHPA-------KYFSIDRVFRNETVDATHLAEFHQVE 374
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +1
Query: 598 TERFEVYVAKKEICNAYTELNDPAT 672
+ER E+++ K E CN + NDP++
Sbjct: 217 SEREELFIKKLEQCNIIFDFNDPSS 241
>SPCC16C4.16c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 161
Score = 27.1 bits (57), Expect = 2.9
Identities = 15/50 (30%), Positives = 21/50 (42%)
Frame = +3
Query: 147 NPEFTTCEFYMAYADYNDLMTITESILSGMVQSIHGPTRSNIIQKDQRVR 296
+P TC+F + YA Y D + + L HG R QK + R
Sbjct: 110 HPTVPTCQFKIRYARYGD-KKVKSAHLYSRYYLFHGDPREEKAQKSKSKR 158
>SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual
Length = 545
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -1
Query: 137 VDTFISELSSNFIYPIESTHNKHFVIKLRSYSH 39
+DTF + ++N PI+ST + H V RS S+
Sbjct: 315 IDTFSLDEAANIQIPIQSTSDNHTVTVDRSLSN 347
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +2
Query: 326 ARVPMMATLERVLNVKLPAPDKLDTPEANDFLN 424
AR P+ L++V KLP PD LDT + +D N
Sbjct: 1369 ARYPIFWGLKKV--GKLPNPDPLDTAQLDDPTN 1399
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.2 bits (55), Expect = 5.0
Identities = 17/41 (41%), Positives = 21/41 (51%)
Frame = -3
Query: 582 SMVLREGTHNLRMIQYKCRIYTLLLRNTETNLSKSLAVVLG 460
+MV R HNLR Y CR+Y LR TN + + LG
Sbjct: 678 AMVPRLSKHNLR---YLCRVYPGPLRVGSTNFNPQVYWRLG 715
>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 555
Score = 26.2 bits (55), Expect = 5.0
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +3
Query: 24 NMDLYMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGI 134
N+D+ MRI P L + DRVY +G RNE I
Sbjct: 430 NLDIRMRITPLLTG----IPQPDRVYVVGGASRNEAI 462
>SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 183
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/41 (34%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = -2
Query: 370 HVKNTFQCCHHR-YSSKGWCEIDFYFLTL-WSFWMIFDLVG 254
H T CC ++ K W + FYF L WM D+ G
Sbjct: 121 HAFTTTWCCMFELFAEKKWMIMSFYFPYLAIPLWMAIDMGG 161
>SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 577
Score = 25.4 bits (53), Expect = 8.7
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = -1
Query: 536 INVGFIHSSSEIQKPIYPRASRWSSV 459
I++ F HS + + +PIY ++ W V
Sbjct: 499 IDLEFDHSKATVNEPIYSKSDGWIPV 524
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,306,698
Number of Sequences: 5004
Number of extensions: 71907
Number of successful extensions: 224
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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