Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0932.Seq
(713 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 51 2e-07
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 42 1e-04
SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces p... 30 0.29
SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|ch... 28 1.5
SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4 |Schi... 27 3.5
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 27 3.5
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 4.7
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 26 6.1
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 8.1
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 25 8.1
SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosacch... 25 8.1
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 50.8 bits (116), Expect = 2e-07
Identities = 20/34 (58%), Positives = 28/34 (82%)
Frame = -2
Query: 625 ALASTGRDINWDMKRLEGYRNFCNKLWNASRFVL 524
+L + GRD+N D+ R+EGYR FCNKL+NA++F L
Sbjct: 718 SLTTGGRDLNLDILRVEGYRKFCNKLYNATKFAL 751
Score = 48.8 bits (111), Expect = 8e-07
Identities = 22/74 (29%), Positives = 39/74 (52%)
Frame = -3
Query: 474 SLADRWILAEFNQTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEA 295
SL ++WI N A + ++ F A +++F + CD Y+E +K +++ GTE
Sbjct: 771 SLVEKWIFHRLNIAAAAMNKNMEEMNFLQATSAVHQFWLYELCDVYIENSKYLLSDGTEV 830
Query: 294 ELRGTRHTLVTVLE 253
+ + TL TVL+
Sbjct: 831 QQESAKQTLYTVLD 844
Score = 42.7 bits (96), Expect = 5e-05
Identities = 23/70 (32%), Positives = 35/70 (50%)
Frame = -2
Query: 247 LRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTEWLKQAIVA 68
LRL HP +P++TE +WQR+ G TI+ FP +E A E + + +
Sbjct: 847 LRLMHPFMPYVTEEMWQRLPRRPGDKTQTIVKAAFPVERVDYSNEIAAKYYESIITVVHS 906
Query: 67 VRNIRAEMNI 38
R++ AE I
Sbjct: 907 TRSMMAENGI 916
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 41.5 bits (93), Expect = 1e-04
Identities = 25/76 (32%), Positives = 38/76 (50%), Gaps = 5/76 (6%)
Frame = -2
Query: 250 LLRLAHPIIPFITETIWQRVKVLCGITADTIMLQ-----PFPQYDASQVDEAALADTEWL 86
L+R+ P PFITE I+Q ++ I + I L+ PFP Y + DE L + +
Sbjct: 761 LVRIMGPFTPFITENIYQHLRNYMPIDKNEISLRSVHFLPFPTYKSELDDETVLRRVKRM 820
Query: 85 KQAIVAVRNIRAEMNI 38
+ I R +R + NI
Sbjct: 821 QTIIELARYVREQNNI 836
>SPAC17A2.06c |vps8||WD repeat protein Vps8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1272
Score = 30.3 bits (65), Expect = 0.29
Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
Frame = -3
Query: 462 RWILAEFNQTI-KAYREALDSFRFDIAAGILYEFTWNQFCDWYLELT 325
+++L +F + I K + ++ S R + I+YE T+ Q WY E T
Sbjct: 959 KYLLDDFTKVITKTF--SIKSIRLQFLSLIIYEITYEQLSSWYRERT 1003
>SPBC2F12.03c |||EST1 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 891
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +1
Query: 427 FDGLVELRQNPAVRQRQVISPPLKPQSYLLCSSAQSG 537
FD LVE RQ ++ V+ P L P L S Q+G
Sbjct: 555 FDDLVERRQYGSLSSNAVLMPMLLPLCVLTSSCLQNG 591
>SPAC22A12.03c |csn4||COP9/signalosome complex subunit Csn4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 377
Score = 26.6 bits (56), Expect = 3.5
Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +1
Query: 250 DLQYSHQR-MASTAQFCFGATVHYRLGQLEIPVTELVPGELIQNACGDIEAEAVQRFAVR 426
+LQ + R + T QF A +YR+ Q ++P EL+ E + + + ++
Sbjct: 176 ELQLCNARALDETGQFLEAAKCYYRVLQYKVPGNELIYRENLCSVAQCLLLAIPSPIVLQ 235
Query: 427 FDGLVELRQNPAVRQ 471
F L E+ P+VR+
Sbjct: 236 F--LQEISLQPSVRE 248
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 26.6 bits (56), Expect = 3.5
Identities = 13/58 (22%), Positives = 29/58 (50%)
Frame = +2
Query: 284 PRSSASVPPFITGLVSSRYQSQNWFQVNSYRMPAAISKRKLSSASRYALMVWLNSARI 457
P++ AS PP + +S ++++ +++ + + +S SRY +M+ N I
Sbjct: 306 PKARASTPPPLNFSRASEHRNEKGERISMINPRVVLDENGISHRSRYFIMLCDNETAI 363
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 26.2 bits (55), Expect = 4.7
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = -3
Query: 516 QKVGLRLQRRRNDLSLADRWILAEFNQTIKAYREALDSFR 397
+K +Q R+++ ++ D+ A+F I AY++ +D R
Sbjct: 716 EKQRKEVQERKHEQAIKDKKAFAQFASYIHAYKQNIDDER 755
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -1
Query: 704 KIRKRTEKQFPNGIEPHGTD 645
K K+ EK FPNGI G D
Sbjct: 595 KSSKQMEKAFPNGIPAQGID 614
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 25.4 bits (53), Expect = 8.1
Identities = 19/60 (31%), Positives = 26/60 (43%)
Frame = -3
Query: 444 FNQTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV 265
F+Q K +L S IA I+ T+ + LTK +NGG R R+ LV
Sbjct: 1381 FDQLSKTVVASLPSIFSLIATWIVLYITFAIAFNQIFGLTKLGLNGGPNKNFRSIRNALV 1440
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +1
Query: 259 YSHQRM-ASTAQFCFGATVHYRLGQLEIPVTELVPGE 366
+ H RM S + CF +++ + + IPVTE+V E
Sbjct: 225 FLHGRMYLSESHICFNSSIFGWVTNIVIPVTEIVSVE 261
>SPBC215.08c |arg4||carbamoyl-phosphate synthase
Arg4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1160
Score = 25.4 bits (53), Expect = 8.1
Identities = 19/73 (26%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = -3
Query: 483 NDLSLADRWILAEFNQTIKAYREALD-SFRFDIAAGILYEFTWNQFCDWYLELTKPVMNG 307
+DL+ D+W L++ K Y+E + + + I+ F D L+++K V G
Sbjct: 522 HDLTKIDKWFLSKLANMAKVYKELEEIGSLYGLNKEIMLRAKKTGFSD--LQISKLV--G 577
Query: 306 GTEAELRGTRHTL 268
+E ++R R L
Sbjct: 578 ASELDVRARRKRL 590
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,795,291
Number of Sequences: 5004
Number of extensions: 54809
Number of successful extensions: 199
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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