Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0896.Seq
(726 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NA... 112 2e-25
At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NA... 109 2e-24
At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NA... 107 7e-24
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA... 100 1e-21
At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NA... 99 1e-21
At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NA... 94 1e-19
At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenas... 67 1e-11
At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /... 30 1.4
At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /... 30 1.4
At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /... 30 1.4
At3g01200.1 68416.m00026 expressed protein contains Pfam domain ... 29 2.4
At5g10500.1 68418.m01216 kinase interacting family protein simil... 29 4.1
At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) fami... 29 4.1
At1g47790.1 68414.m05317 F-box family protein contains Pfam:PF00... 29 4.1
At3g29350.2 68416.m03686 two-component phosphorelay mediator 1 (... 28 5.5
At3g29350.1 68416.m03685 two-component phosphorelay mediator 1 (... 28 5.5
At3g19190.1 68416.m02436 expressed protein 28 7.2
At1g28320.1 68414.m03475 protease-related similar to Protease de... 28 7.2
At2g28290.2 68415.m03434 chromatin remodeling protein, putative ... 27 9.6
At2g28290.1 68415.m03433 chromatin remodeling protein, putative ... 27 9.6
>At4g35650.1 68417.m05062 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 368
Score = 112 bits (270), Expect = 2e-25
Identities = 68/158 (43%), Positives = 95/158 (60%), Gaps = 1/158 (0%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 434
P + AV++V +A PV FE + EV + E+V+ S+ NK+C+KG LATP
Sbjct: 51 PLVTGAVEQVMEAMHAPVHFERY---EVLGNMRKVPEEVIESVKRNKVCLKGGLATPV-- 105
Query: 435 HTGELQTLNMKLRNALDLYANVVQ*V-IAQREVQAPGRGLHHHQRTDRREYSALEHESVP 611
G + +LNM+LR LD++A++V + + + + + EYS LEHE VP
Sbjct: 106 -GGGVSSLNMQLRKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVP 164
Query: 612 GVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
GVVE LK+IT SERIA++AF+YA RKKVTAVHK
Sbjct: 165 GVVESLKVITKFCSERIARYAFEYAYLNNRKKVTAVHK 202
>At2g17130.1 68415.m01977 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 367
Score = 109 bits (262), Expect = 2e-24
Identities = 72/164 (43%), Positives = 93/164 (56%), Gaps = 7/164 (4%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 434
P + AVQ+V +A PV FE F EV+ + + E ++ SI NK+C+KG L TP
Sbjct: 50 PLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKKNKVCLKGGLKTPV-- 104
Query: 435 HTGELQTLNMKLRNALDLYANVVQ*VIAQREVQAPGRGLHHH-------QRTDRREYSAL 593
G + +LN+ LR LDL+A++V PG H + EY+ L
Sbjct: 105 -GGGVSSLNVNLRKELDLFASLVNCF------NLPGLASRHENVDIVVIRENTEGEYAGL 157
Query: 594 EHESVPGVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
EHE VPGVVE LK+IT SERIAK+AF+YA RKKVTAVHK
Sbjct: 158 EHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHK 201
>At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NAD+
isocitrate dehydrogenase subunit 1 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 367
Score = 107 bits (257), Expect = 7e-24
Identities = 66/158 (41%), Positives = 94/158 (59%), Gaps = 1/158 (0%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 434
P + AV++V +A P+ FE + +V+ +S +V+ SI NK+C+KG L TP
Sbjct: 50 PLVTNAVEQVMEAMHAPIFFEKY---DVHGEMSRVPPEVMESIRKNKVCLKGGLKTPV-- 104
Query: 435 HTGELQTLNMKLRNALDLYANVVQ*V-IAQREVQAPGRGLHHHQRTDRREYSALEHESVP 611
G + +LN++LR LDL+A++V + + + + EY+ LEHE VP
Sbjct: 105 -GGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVP 163
Query: 612 GVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
GVVE LK+IT SERIAK+AF+YA RKKVTAVHK
Sbjct: 164 GVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHK 201
>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
[Nicotiana tabacum]
Length = 374
Score = 100 bits (239), Expect = 1e-21
Identities = 66/160 (41%), Positives = 96/160 (60%), Gaps = 3/160 (1%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDF-ESFFFSEVNPTLSAPLE-DVVNSIAVNKICIKGILATPD 428
PE+ +V++VF AA + +D+ E F +EV+P ++ L D + S+ NK+ +KG +ATP
Sbjct: 56 PEIAESVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATP- 114
Query: 429 FSHTGELQTLNMKLRNALDLYANVVQ*V-IAQREVQAPGRGLHHHQRTDRREYSALEHES 605
G ++LN+ LR L+LYANV + + + L + EYS LEH+
Sbjct: 115 -IGKGH-RSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQV 172
Query: 606 VPGVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
V GVVE LKIIT S R+A++AF YA GRKKV+A+HK
Sbjct: 173 VKGVVESLKIITRKASMRVAEYAFLYAKTHGRKKVSAIHK 212
>At2g17130.2 68415.m01978 isocitrate dehydrogenase subunit 2 / NAD+
isocitrate dehydrogenase subunit 2 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana] GI:1766048
Length = 363
Score = 99 bits (238), Expect = 1e-21
Identities = 70/164 (42%), Positives = 90/164 (54%), Gaps = 7/164 (4%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 434
P + AVQ+V +A PV FE F EV+ + + E ++ SI NK+C+KG L TP
Sbjct: 50 PLVTNAVQQVMEAMHAPVYFEPF---EVHGDMKSLPEGLLESIKKNKVCLKGGLKTPV-- 104
Query: 435 HTGELQTLNMKLRNALDLYANVVQ*VIAQREVQAPGRGLHHH-------QRTDRREYSAL 593
G + +LN+ LR LDL+A++V PG H + EY+ L
Sbjct: 105 -GGGVSSLNVNLRKELDLFASLVNCF------NLPGLASRHENVDIVVIRENTEGEYAGL 157
Query: 594 EHESVPGVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
EHE VPGVVE LK SERIAK+AF+YA RKKVTAVHK
Sbjct: 158 EHEVVPGVVESLKFC----SERIAKYAFEYAYLNNRKKVTAVHK 197
>At5g03290.1 68418.m00279 isocitrate dehydrogenase, putative / NAD+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
GI:3021506
Length = 374
Score = 93.9 bits (223), Expect = 1e-19
Identities = 60/160 (37%), Positives = 95/160 (59%), Gaps = 3/160 (1%)
Frame = +3
Query: 255 PELVYAVQEVFKAASIPVDFESFFF-SEVNPTLSAPLE-DVVNSIAVNKICIKGILATPD 428
PE+ +V++VF A +P+++E + +E++P + L + + S+ NK+ +KG +ATP
Sbjct: 56 PEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATP- 114
Query: 429 FSHTGELQTLNMKLRNALDLYANVVQ*V-IAQREVQAPGRGLHHHQRTDRREYSALEHES 605
G ++LN+ LR L+LYANV + + + L + EYS LEH+
Sbjct: 115 -IGKGH-RSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQV 172
Query: 606 VPGVVECLKIITAAKSERIAKFAFDYAVKMGRKKVTAVHK 725
V GVVE LKIIT S R+A++AF YA GR++V+A+HK
Sbjct: 173 VRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHK 212
>At1g32480.1 68414.m04008 isocitrate/isopropylmalate dehydrogenase
family protein similar to NAD+ dependent isocitrate
dehydrogenase subunit 2 [Arabidopsis thaliana]
GI:1766048; contains Pfam profile PF00180 dehydrogenase,
isocitrate/isopropylmalate family
Length = 294
Score = 66.9 bits (156), Expect = 1e-11
Identities = 52/153 (33%), Positives = 79/153 (51%), Gaps = 2/153 (1%)
Frame = +3
Query: 270 AVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGEL 449
AV +V A PV FE++ N ++ +VV+SI NK+C+ G +
Sbjct: 15 AVHQVMDAMQAPVYFETYIIKGKN--MNHLTWEVVDSIRKNKVCLNGRVNN--------- 63
Query: 450 QTLNMKLRNALDLYANVVQ*V-IAQREVQAPGRGLHHHQRTDRREYSALEHESVPGVVEC 626
+L R LDL+A++V + + + + + EY+ EHE VPGV+E
Sbjct: 64 -SLCGGARKELDLFASLVDCFNLNGQPSRHENVDIVVIRENTEGEYAGREHEVVPGVIES 122
Query: 627 LKI-ITAAKSERIAKFAFDYAVKMGRKKVTAVH 722
++ +T S+RIAK+AF+YA RKKVTAVH
Sbjct: 123 FQVTMTKFWSDRIAKYAFEYAHFSKRKKVTAVH 155
>At1g14820.3 68414.m01773 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 252
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 261 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 410
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 35 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 84
>At1g14820.2 68414.m01772 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 261 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 410
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At1g14820.1 68414.m01771 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; similar to
phosphatidylinositol/phosphatidylcholine transfer
protein SP:P24280 [Saccharomyces cerevisiae (Baker's
yeast)]
Length = 239
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 261 LVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKG 410
LV + KAA + VD++ + S V PT P +V + + K+C++G
Sbjct: 22 LVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQG 71
>At3g01200.1 68416.m00026 expressed protein contains Pfam domain
PF03618: Domain of unknown function (DUF299)
Length = 377
Score = 29.5 bits (63), Expect = 2.4
Identities = 18/80 (22%), Positives = 35/80 (43%)
Frame = +3
Query: 204 NKGRSYQMHLDSWGRCRPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSI 383
N+G S HL SW L+ +++ K ++ F++ NP+++ + + +
Sbjct: 117 NRGSSVNTHLFSWVEDEERLIEIIKQAAKEGAM------CFYTLANPSMAKSAKQACDQL 170
Query: 384 AVNKICIKGILATPDFSHTG 443
V + I G + SH G
Sbjct: 171 GVLSVDILGPIIEGIASHLG 190
>At5g10500.1 68418.m01216 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) [Petunia
integrifolia]
Length = 848
Score = 28.7 bits (61), Expect = 4.1
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = +3
Query: 204 NKGRSYQMHLDSWGRCRPELVYAVQEVFK---AASIPVDFESFFFSEVNPTLSAPLEDVV 374
++G S+ + + + RPEL+ V+E FK A + D S N T+++ D V
Sbjct: 46 DEGDSFAKRAEMYYKRRPELISFVEESFKAYRALAERYDHISKELQNANTTIASVFPDQV 105
Query: 375 NSIAVNK 395
A+N+
Sbjct: 106 PEFAMNE 112
>At4g17610.1 68417.m02633 tRNA/rRNA methyltransferase (SpoU) family
protein similar to TAR RNA loop binding protein [Homo
sapiens] GI:1184692; contains Pfam profile PF00588: SpoU
rRNA Methylase (RNA methyltransferase, TrmH) family
Length = 1850
Score = 28.7 bits (61), Expect = 4.1
Identities = 20/63 (31%), Positives = 31/63 (49%)
Frame = +3
Query: 195 TEGNKGRSYQMHLDSWGRCRPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVV 374
T G RS + L SW E + +V + A IP D +SFF + T+ + +D+V
Sbjct: 1057 TAGVLVRSRRAVLLSWKWLCVESLLSVMHILDARRIPEDRKSFFSDD---TVKSIFQDIV 1113
Query: 375 NSI 383
S+
Sbjct: 1114 ESL 1116
>At1g47790.1 68414.m05317 F-box family protein contains Pfam:PF00646
F-box domain
Length = 389
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/97 (23%), Positives = 41/97 (42%), Gaps = 3/97 (3%)
Frame = +3
Query: 159 EKCMLCTIWSPATEGNKGRSYQMHL---DSWGRCRPELVYAVQEVFKAASIPVDFESFFF 329
++C + T+ S ++ H D+WGRC +VY + V+ +
Sbjct: 185 DECQVLTLGSAQKSWRTVKTKHKHRSTNDTWGRCIKGVVYYIAYVYH-TRVWCIMSFHVK 243
Query: 330 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHT 440
SE + PLE++ + +N +G LA D +T
Sbjct: 244 SEKFDMIKLPLENIYRDVMIN---YEGRLACVDKLYT 277
>At3g29350.2 68416.m03686 two-component phosphorelay mediator 1
(HP1) identical to ATHP1 [Arabidopsis thaliana]
GI:4156241
Length = 114
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = -1
Query: 270 RRQAQAYTVPR-NQGAFDTTFLCCPRLQ--GSKWCITYIFLCCYRTCVYTLSNMLRSL 106
+RQ + YT+ QG D F +LQ GS ++ + + CV +SNM R+L
Sbjct: 9 QRQFRDYTISLYQQGFLDDQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARAL 66
>At3g29350.1 68416.m03685 two-component phosphorelay mediator 1
(HP1) identical to ATHP1 [Arabidopsis thaliana]
GI:4156241
Length = 156
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = -1
Query: 270 RRQAQAYTVPR-NQGAFDTTFLCCPRLQ--GSKWCITYIFLCCYRTCVYTLSNMLRSL 106
+RQ + YT+ QG D F +LQ GS ++ + + CV +SNM R+L
Sbjct: 9 QRQFRDYTISLYQQGFLDDQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARAL 66
>At3g19190.1 68416.m02436 expressed protein
Length = 1861
Score = 27.9 bits (59), Expect = 7.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 193 QPRATKEGRIKCTLIPGDGVGLSLSTLYK 279
QPR K+G +K GDG+G + S L +
Sbjct: 1741 QPRNAKQGMLKACESIGDGIGKTASALVR 1769
>At1g28320.1 68414.m03475 protease-related similar to Protease degS
[Precursor] (SP:P44947) [Haemophilus influenzae];
similar to DegP protease precursor (GI:2565436)
[Arabidopsis thaliana]
Length = 709
Score = 27.9 bits (59), Expect = 7.2
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 228 HLDSWGRCRPELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAP 359
HLDSW C +VY +E A + +++ + S+P
Sbjct: 471 HLDSWTWCPANVVYICKEQLDIALLQLEYVPGKLQPITANFSSP 514
>At2g28290.2 68415.m03434 chromatin remodeling protein, putative (SYD)
similar to transcriptional activator HBRM [Homo sapiens]
GI:414117; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain; identical to cDNA putative chromatin
remodeling protein SYD (SPLAYED) GI:13603720
Length = 3529
Score = 27.5 bits (58), Expect = 9.6
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +3
Query: 330 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVQ 506
S NP +P+ ++ +K K + A P F +QTLN+ L N+ + A V+
Sbjct: 1599 STSNPDQVSPVHSATTALRSDKAADKDLDAPPGFDSGSHVQTLNV-LENSSERKAFAVK 1656
>At2g28290.1 68415.m03433 chromatin remodeling protein, putative (SYD)
similar to transcriptional activator HBRM [Homo sapiens]
GI:414117; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain; identical to cDNA putative chromatin
remodeling protein SYD (SPLAYED) GI:13603720
Length = 3574
Score = 27.5 bits (58), Expect = 9.6
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +3
Query: 330 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVQ 506
S NP +P+ ++ +K K + A P F +QTLN+ L N+ + A V+
Sbjct: 1599 STSNPDQVSPVHSATTALRSDKAADKDLDAPPGFDSGSHVQTLNV-LENSSERKAFAVK 1656
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,566,179
Number of Sequences: 28952
Number of extensions: 374502
Number of successful extensions: 1015
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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