Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0885.Seq
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 143 1e-34
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 143 1e-34
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 143 1e-34
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.67
At1g29760.1 68414.m03639 expressed protein 31 0.67
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.7
At5g63000.1 68418.m07904 expressed protein 28 4.7
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 4.7
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 4.7
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 4.7
At4g20040.1 68417.m02933 expressed protein C70DMY30S 27 8.2
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 143 bits (346), Expect = 1e-34
Identities = 66/88 (75%), Positives = 74/88 (84%)
Frame = +2
Query: 2 LNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTMNCF 265
VDGKVRTD TYPAGFMDVVSI KT F
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENF 99
Score = 122 bits (294), Expect = 2e-28
Identities = 53/85 (62%), Positives = 68/85 (80%)
Frame = +1
Query: 256 ELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKV 435
E FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK
Sbjct: 97 ENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKP 156
Query: 436 NDSIQLDIATTKIMDFIKFESGNLL 510
ND+I+LD+ KI++FIKF+ GN++
Sbjct: 157 NDTIKLDLEANKIVEFIKFDVGNVV 181
Score = 81.0 bits (191), Expect = 6e-16
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +3
Query: 495 VWELVMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 653
V +VM+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 143 bits (346), Expect = 1e-34
Identities = 66/88 (75%), Positives = 74/88 (84%)
Frame = +2
Query: 2 LNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTMNCF 265
VDGKVRTD TYPAGFMDVVSI KT F
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENF 99
Score = 122 bits (293), Expect = 3e-28
Identities = 53/85 (62%), Positives = 68/85 (80%)
Frame = +1
Query: 256 ELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKV 435
E FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK
Sbjct: 97 ENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKP 156
Query: 436 NDSIQLDIATTKIMDFIKFESGNLL 510
ND+I+LD+ KI++FIKF+ GN++
Sbjct: 157 NDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 81.0 bits (191), Expect = 6e-16
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +3
Query: 495 VWELVMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 653
V +VM+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 143 bits (346), Expect = 1e-34
Identities = 66/88 (75%), Positives = 74/88 (84%)
Frame = +2
Query: 2 LNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 181
LNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+
Sbjct: 12 LNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQ 71
Query: 182 VDGKVRTDPTYPAGFMDVVSIEKTMNCF 265
VDGKVRTD TYPAGFMDVVSI KT F
Sbjct: 72 VDGKVRTDKTYPAGFMDVVSIPKTNENF 99
Score = 122 bits (293), Expect = 3e-28
Identities = 53/85 (62%), Positives = 68/85 (80%)
Frame = +1
Query: 256 ELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKV 435
E FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK
Sbjct: 97 ENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKP 156
Query: 436 NDSIQLDIATTKIMDFIKFESGNLL 510
ND+I+LD+ KI++FIKF+ GN++
Sbjct: 157 NDTIKLDLEENKIVEFIKFDVGNVV 181
Score = 81.0 bits (191), Expect = 6e-16
Identities = 35/53 (66%), Positives = 43/53 (81%)
Frame = +3
Query: 495 VWELVMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIG 653
V +VM+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IG
Sbjct: 177 VGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIG 229
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.67
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 93 KHSRNLWGPVDGLGAYTPPSLSNIHAL 13
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.67
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 204 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 112
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 183 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 67
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At5g63000.1 68418.m07904 expressed protein
Length = 201
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/48 (39%), Positives = 23/48 (47%)
Frame = -3
Query: 611 SRGVLDVHNVEGAGMSLAGHDGAHTPQVTASRDHNKFPDSNLMKSIIF 468
+RGV DV NV GAG + A G P A R N S L ++ F
Sbjct: 110 TRGVHDVFNVVGAGSATAAVFGLIMPGSLAWRARNVLLGSVLGATVCF 157
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 116 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 250
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At4g20040.1 68417.m02933 expressed protein C70DMY30S
Length = 483
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/65 (26%), Positives = 29/65 (44%)
Frame = -1
Query: 262 TVHSLLN*DNIHKSSRISRVSPNFPINLYEALFHNFQDFVSGQSILQTIPQENHQGQALA 83
+VH ++ NI + NFPI E FH+ V Q+ + + ++ G+A+
Sbjct: 358 SVHGKISGVNIVNNMFSGTAKNNFPIVKLEGEFHDINQVVIDQNNAEGMMLKSTTGKAMV 417
Query: 82 QLVGT 68
GT
Sbjct: 418 SANGT 422
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,151,482
Number of Sequences: 28952
Number of extensions: 356806
Number of successful extensions: 1040
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1038
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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