BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0869.Seq (588 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g57290.1 68416.m06377 eukaryotic translation initiation facto... 63 1e-10 At3g62050.1 68416.m06971 expressed protein contains Pfam profile... 28 4.0 At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 28 5.3 At5g50990.1 68418.m06322 pentatricopeptide (PPR) repeat-containi... 27 7.0 At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2 ... 27 7.0 >At3g57290.1 68416.m06377 eukaryotic translation initiation factor 3E / eIF3e (TIF3E1) identical to eukaryotic initiation factor 3E subunit [Arabidopsis thaliana] gi|12407658|gb|AAG53613 Length = 441 Score = 63.3 bits (147), Expect = 1e-10 Identities = 26/56 (46%), Positives = 40/56 (71%) Frame = +2 Query: 89 SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVM 256 S +DLT I LDRHLVFP+ EFL ++ Y ++L++K+++L++TNM+DY M Sbjct: 4 SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAM 59 Score = 47.6 bits (108), Expect = 6e-06 Identities = 24/85 (28%), Positives = 48/85 (56%) Frame = +1 Query: 256 DIRRMLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMKTIETMRDPKTLI 435 DI + LY ED P+E+++RR V++ L+ L++A P++ + + ++ +R K Sbjct: 60 DIHKSLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPN---AVQELRADKQ-Y 115 Query: 436 NHLSTNKEYEFKIEMIDSMYRLAKF 510 N + Y+ + I+++Y+ AKF Sbjct: 116 NLQMLKERYQIGPDQIEALYQYAKF 140 Score = 28.3 bits (60), Expect = 4.0 Identities = 9/19 (47%), Positives = 14/19 (73%) Frame = +3 Query: 498 FGQIRYECGNYVESASYLY 554 + + ++ECGNY +A YLY Sbjct: 137 YAKFQFECGNYSGAADYLY 155 >At3g62050.1 68416.m06971 expressed protein contains Pfam profile PF04396: Protein of unknown function, DUF537 Length = 157 Score = 28.3 bits (60), Expect = 4.0 Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Frame = -1 Query: 351 ILKFLQFRKNYTSPLYNFFRSVFF-RIEHSSDVIT*SIMLV 232 +++ L+ +KNY +YNF V++ + EHS SI+L+ Sbjct: 117 VVQLLKSKKNYNFIIYNFILYVYYQKTEHSYLAPIPSILLI 157 >At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563; metal transport capacity has not been shown, PMID:11500563, PMID:1038174 Length = 1294 Score = 27.9 bits (59), Expect = 5.3 Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 1/53 (1%) Frame = -1 Query: 540 LTPHNFHIHSEF-GQTIHAIDHFNFKLILLVCRKMVYKSFRIPHGFNCFHYII 385 + PHNF+IHS F G++ + D L C+ ++ F + G + +Y++ Sbjct: 207 IVPHNFYIHSYFAGESTSSSDVDKSSL----CQDHLFAIFGVFSGLSLVNYVL 255 >At5g50990.1 68418.m06322 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 511 Score = 27.5 bits (58), Expect = 7.0 Identities = 15/40 (37%), Positives = 21/40 (52%) Frame = +2 Query: 167 LAAKETYDQSELLQAKLEILSKTNMIDYVMTSEECSILKK 286 L++ TY EL + ++ LSK DYV+ S S KK Sbjct: 318 LSSSRTYKNPELGEIAIQNLSKAKSGDYVLLSNIYSSTKK 357 >At1g36070.1 68414.m04484 WD-40 repeat family protein contains 2 WD-40 repeats (PF0400);similar to guanine nucleotide-binding protein beta subunit GPBA (SP:P36408) [Dictyostelium discoideum (Slime mold)]; similar to katanin p80 (WD40-containing) subunit B 1 (GI:12655011) [Homo sapiens] Length = 418 Score = 27.5 bits (58), Expect = 7.0 Identities = 14/59 (23%), Positives = 30/59 (50%), Gaps = 1/59 (1%) Frame = +1 Query: 346 QDAVEPVLRLMQRDD-VMKTIETMRDPKTLINHLSTNKEYEFKIEMIDSMYRLAKFAMN 519 Q V RL D+ + T++ RDP + ++ N + + ++ S R+++FA++ Sbjct: 190 QPGVAFCTRLSTEDNAITNTVDIYRDPSGSLRLITANNDCKIRVFDAQSFTRVSEFALD 248 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,748,689 Number of Sequences: 28952 Number of extensions: 218814 Number of successful extensions: 605 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 597 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 605 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1161268208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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