BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0860X.Seq (434 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g36340.1 68414.m04516 ubiquitin-conjugating enzyme family pro... 28 2.4 At5g42840.1 68418.m05221 DC1 domain-containing protein contains ... 27 4.1 At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr... 27 4.1 At1g33410.1 68414.m04136 expressed protein 27 5.5 At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos... 27 7.2 At3g59770.1 68416.m06670 sacI homology domain-containing protein... 26 9.5 At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 26 9.5 At1g45110.1 68414.m05171 tetrapyrrole methylase family protein c... 26 9.5 >At1g36340.1 68414.m04516 ubiquitin-conjugating enzyme family protein similar to Ubiquitin-conjugating enzyme E2-16 kDa (EC 6.3.2.19) (Ubiquitin-protein ligase) (Ubiquitin carrier protein) from {Schizosaccharomyces pombe} SP|P46595, {Caenorhabditis elegans} SP|P35129; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 154 Score = 28.3 bits (60), Expect = 2.4 Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 3/66 (4%) Frame = -2 Query: 319 EPLFSISSRGNPLFPTTSNSWSPQGSVLSPFLFSL---FVNDIPRSPPTNLALFADDTTV 149 E LFSI N ++ T+ P G+ +F+L F D P PP F T + Sbjct: 24 ESLFSIGQNSNNIYEWTAVIRGPDGTPYEGGMFNLSIKFPTDYPFKPPK----FTFKTPI 79 Query: 148 YYSSRN 131 Y+ + N Sbjct: 80 YHPNIN 85 >At5g42840.1 68418.m05221 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 671 Score = 27.5 bits (58), Expect = 4.1 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = -3 Query: 429 FDVAKAFDKVWHNGLIFKLFNMGVPD 352 FD+ + FD H+G KL G PD Sbjct: 51 FDIQEIFDHPSHDGHCLKLLTTGAPD 76 >At5g14460.1 68418.m01692 pseudouridylate synthase TruB family protein similar to SP|P09171 tRNA pseudouridine synthase B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli O157:H7}; contains Pfam profile PF01509: TruB family pseudouridylate synthase (N terminal domain) Length = 540 Score = 27.5 bits (58), Expect = 4.1 Identities = 19/73 (26%), Positives = 33/73 (45%) Frame = -2 Query: 313 LFSISSRGNPLFPTTSNSWSPQGSVLSPFLFSLFVNDIPRSPPTNLALFADDTTVYYSSR 134 L S S+R + P +S P ++ P+L SLF++ P + + T S+ Sbjct: 10 LMSSSARMSMTSPLFFSSVKPWRNIHKPYLASLFLSTTSTRYPLQYDMIINRPTQSSLSQ 69 Query: 133 NKSLIAKKLQSAA 95 N+ K ++S A Sbjct: 70 NRRRPPKAIESGA 82 >At1g33410.1 68414.m04136 expressed protein Length = 1459 Score = 27.1 bits (57), Expect = 5.5 Identities = 14/35 (40%), Positives = 21/35 (60%) Frame = +3 Query: 189 GDRGISLTNKLNRKGERTEPCGLQLLEVVGKSGFP 293 G+ IS ++N+ T P L+LL++ KSGFP Sbjct: 66 GEPSISFVWRINK----TSPNALELLQLSAKSGFP 96 >At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative similar to SP:P24859 from [Kluyveromyces lactissimilar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 608 Score = 26.6 bits (56), Expect = 7.2 Identities = 16/61 (26%), Positives = 26/61 (42%), Gaps = 2/61 (3%) Frame = -2 Query: 412 VRQSLAQWFDFQTIQHGRAG*SRAHHTGLLVEPLFSISSRGNPLF--PTTSNSWSPQGSV 239 +R W D + ++ GR+G + H G + ISS P + + S + GS Sbjct: 325 MRSDKGPWKDSEILKMGRSGGTFCRHAGAFLSSDSQISSSDKPTYSLKVSDTSTAKSGSE 384 Query: 238 L 236 L Sbjct: 385 L 385 >At3g59770.1 68416.m06670 sacI homology domain-containing protein / WW domain-containing protein contains Pfam profiles PF00397: WW domain, PF02383: SacI homology domain; identical to cDNA SAC domain protein 9 (SAC9) GI:31415734 Length = 1630 Score = 26.2 bits (55), Expect = 9.5 Identities = 10/32 (31%), Positives = 18/32 (56%) Frame = -3 Query: 402 VWHNGLIFKLFNMGVPDSLVLIIRDFLSNRSF 307 VW+ L N+G+P+ V++++ F R F Sbjct: 182 VWNRWLSVPFKNIGLPEHCVILLQGFAEYRPF 213 >At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family protein low similarity to dTDP-D-glucose-4,6-dehydratase from Aneurinibacillus thermoaerophilus GI:16357461, Sphingomonas sp. GI:1314581; contains Pfam profile PF01370: NAD dependent epimerase/dehydratase family; putative NDP-rhamnose synthase (rhm2 gene) GI:31559258 Length = 667 Score = 26.2 bits (55), Expect = 9.5 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = -3 Query: 426 DVAKAFDKVWHNGLIFKLFNMG 361 DVA+AF+ V H G I ++N+G Sbjct: 232 DVAEAFEVVLHKGEIGHVYNVG 253 >At1g45110.1 68414.m05171 tetrapyrrole methylase family protein contains Pfam PF00590 : Tetrapyrrole (Corrin/Porphyrin) Methylases domain; contains TIGRFAMS TIGR00522 : diphthine synthase Length = 343 Score = 26.2 bits (55), Expect = 9.5 Identities = 9/28 (32%), Positives = 18/28 (64%) Frame = -2 Query: 190 PPTNLALFADDTTVYYSSRNKSLIAKKL 107 PP L+ F ++TT Y+ + +IA+++ Sbjct: 214 PPHKLSQFLEETTPYFGESRQCVIAREI 241 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,373,703 Number of Sequences: 28952 Number of extensions: 185491 Number of successful extensions: 612 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 598 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 612 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 683042040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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