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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0860X.Seq
         (434 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g36340.1 68414.m04516 ubiquitin-conjugating enzyme family pro...    28   2.4  
At5g42840.1 68418.m05221 DC1 domain-containing protein contains ...    27   4.1  
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr...    27   4.1  
At1g33410.1 68414.m04136 expressed protein                             27   5.5  
At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative / phos...    27   7.2  
At3g59770.1 68416.m06670 sacI homology domain-containing protein...    26   9.5  
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam...    26   9.5  
At1g45110.1 68414.m05171 tetrapyrrole methylase family protein c...    26   9.5  

>At1g36340.1 68414.m04516 ubiquitin-conjugating enzyme family
           protein similar to  Ubiquitin-conjugating enzyme E2-16
           kDa (EC 6.3.2.19) (Ubiquitin-protein ligase) (Ubiquitin
           carrier protein) from {Schizosaccharomyces pombe}
           SP|P46595, {Caenorhabditis elegans} SP|P35129; contains
           Pfam profile PF00179: Ubiquitin-conjugating enzyme
          Length = 154

 Score = 28.3 bits (60), Expect = 2.4
 Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 3/66 (4%)
 Frame = -2

Query: 319 EPLFSISSRGNPLFPTTSNSWSPQGSVLSPFLFSL---FVNDIPRSPPTNLALFADDTTV 149
           E LFSI    N ++  T+    P G+     +F+L   F  D P  PP     F   T +
Sbjct: 24  ESLFSIGQNSNNIYEWTAVIRGPDGTPYEGGMFNLSIKFPTDYPFKPPK----FTFKTPI 79

Query: 148 YYSSRN 131
           Y+ + N
Sbjct: 80  YHPNIN 85


>At5g42840.1 68418.m05221 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 671

 Score = 27.5 bits (58), Expect = 4.1
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -3

Query: 429 FDVAKAFDKVWHNGLIFKLFNMGVPD 352
           FD+ + FD   H+G   KL   G PD
Sbjct: 51  FDIQEIFDHPSHDGHCLKLLTTGAPD 76


>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
           protein similar to SP|P09171 tRNA pseudouridine synthase
           B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
           (Pseudouridylate synthase) (Uracil hydrolyase)
           {Escherichia coli O157:H7}; contains Pfam profile
           PF01509: TruB family pseudouridylate synthase (N
           terminal domain)
          Length = 540

 Score = 27.5 bits (58), Expect = 4.1
 Identities = 19/73 (26%), Positives = 33/73 (45%)
 Frame = -2

Query: 313 LFSISSRGNPLFPTTSNSWSPQGSVLSPFLFSLFVNDIPRSPPTNLALFADDTTVYYSSR 134
           L S S+R +   P   +S  P  ++  P+L SLF++      P    +  +  T    S+
Sbjct: 10  LMSSSARMSMTSPLFFSSVKPWRNIHKPYLASLFLSTTSTRYPLQYDMIINRPTQSSLSQ 69

Query: 133 NKSLIAKKLQSAA 95
           N+    K ++S A
Sbjct: 70  NRRRPPKAIESGA 82


>At1g33410.1 68414.m04136 expressed protein
          Length = 1459

 Score = 27.1 bits (57), Expect = 5.5
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = +3

Query: 189 GDRGISLTNKLNRKGERTEPCGLQLLEVVGKSGFP 293
           G+  IS   ++N+    T P  L+LL++  KSGFP
Sbjct: 66  GEPSISFVWRINK----TSPNALELLQLSAKSGFP 96


>At1g19650.1 68414.m02449 SEC14 cytosolic factor, putative /
           phosphoglyceride transfer protein, putative similar to
           SP:P24859 from [Kluyveromyces lactissimilar to
           phosphatidylinositol transfer-like protein IV
           (GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
           CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
           N-terminus
          Length = 608

 Score = 26.6 bits (56), Expect = 7.2
 Identities = 16/61 (26%), Positives = 26/61 (42%), Gaps = 2/61 (3%)
 Frame = -2

Query: 412 VRQSLAQWFDFQTIQHGRAG*SRAHHTGLLVEPLFSISSRGNPLF--PTTSNSWSPQGSV 239
           +R     W D + ++ GR+G +   H G  +     ISS   P +    +  S +  GS 
Sbjct: 325 MRSDKGPWKDSEILKMGRSGGTFCRHAGAFLSSDSQISSSDKPTYSLKVSDTSTAKSGSE 384

Query: 238 L 236
           L
Sbjct: 385 L 385


>At3g59770.1 68416.m06670 sacI homology domain-containing protein /
           WW domain-containing protein contains Pfam profiles
           PF00397: WW domain, PF02383: SacI homology domain;
           identical to cDNA SAC domain protein 9 (SAC9)
           GI:31415734
          Length = 1630

 Score = 26.2 bits (55), Expect = 9.5
 Identities = 10/32 (31%), Positives = 18/32 (56%)
 Frame = -3

Query: 402 VWHNGLIFKLFNMGVPDSLVLIIRDFLSNRSF 307
           VW+  L     N+G+P+  V++++ F   R F
Sbjct: 182 VWNRWLSVPFKNIGLPEHCVILLQGFAEYRPF 213


>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
           protein low similarity to dTDP-D-glucose-4,6-dehydratase
           from Aneurinibacillus thermoaerophilus GI:16357461,
           Sphingomonas sp. GI:1314581; contains Pfam profile
           PF01370: NAD dependent epimerase/dehydratase family;
           putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
          Length = 667

 Score = 26.2 bits (55), Expect = 9.5
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = -3

Query: 426 DVAKAFDKVWHNGLIFKLFNMG 361
           DVA+AF+ V H G I  ++N+G
Sbjct: 232 DVAEAFEVVLHKGEIGHVYNVG 253


>At1g45110.1 68414.m05171 tetrapyrrole methylase family protein
           contains Pfam PF00590 : Tetrapyrrole (Corrin/Porphyrin)
           Methylases domain; contains TIGRFAMS TIGR00522 :
           diphthine synthase
          Length = 343

 Score = 26.2 bits (55), Expect = 9.5
 Identities = 9/28 (32%), Positives = 18/28 (64%)
 Frame = -2

Query: 190 PPTNLALFADDTTVYYSSRNKSLIAKKL 107
           PP  L+ F ++TT Y+    + +IA+++
Sbjct: 214 PPHKLSQFLEETTPYFGESRQCVIAREI 241


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,373,703
Number of Sequences: 28952
Number of extensions: 185491
Number of successful extensions: 612
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 598
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 612
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 683042040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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