Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0853.Seq
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 143 2e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 83 5e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 62 5e-11
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 28 1.4
SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|... 27 1.8
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 26 4.2
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.5
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 5.5
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 26 5.5
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 25 7.3
SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 25 7.3
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 25 9.6
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 9.6
SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ... 25 9.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 143 bits (346), Expect = 2e-35
Identities = 62/85 (72%), Positives = 76/85 (89%)
Frame = +3
Query: 255 AKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCD 434
A NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV AVC
Sbjct: 296 ANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCS 355
Query: 435 IPPRGLKMAATFIGNSTAIQELFKR 509
+PP+ LKM+ATFIGNST+IQE+F+R
Sbjct: 356 VPPKDLKMSATFIGNSTSIQEIFRR 380
Score = 132 bits (319), Expect = 4e-32
Identities = 59/82 (71%), Positives = 71/82 (86%)
Frame = +1
Query: 10 TLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL 189
TLK+ +P+Y DLNHLVS M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFAPL
Sbjct: 214 TLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPL 273
Query: 190 TSRGSRQYRALTVPELTQQMFE 255
+ GS ++A++VPELTQQMF+
Sbjct: 274 AAIGSSSFQAVSVPELTQQMFD 295
Score = 101 bits (241), Expect = 1e-22
Identities = 44/48 (91%), Positives = 47/48 (97%)
Frame = +2
Query: 509 ISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA 652
+ +QF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA
Sbjct: 381 LGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA 428
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 82.6 bits (195), Expect = 5e-17
Identities = 35/86 (40%), Positives = 53/86 (61%)
Frame = +1
Query: 1 CFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGF 180
C R L + PTY +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +
Sbjct: 217 CRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTY 276
Query: 181 APLTSRGSRQYRALTVPELTQQMFEP 258
+P+ S + + +V E+T Q FEP
Sbjct: 277 SPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 58.4 bits (135), Expect = 8e-10
Identities = 27/91 (29%), Positives = 47/91 (51%), Gaps = 8/91 (8%)
Frame = +3
Query: 261 NMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIP 440
N M CDPR GRY+ ++RG + ++V + +I+++ + FV+W P K +C P
Sbjct: 304 NQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEP 363
Query: 441 PR--------GLKMAATFIGNSTAIQELFKR 509
P+ + A + N+T+I E + R
Sbjct: 364 PQHVPGSGIAKVNRAVCMLSNTTSIAEAWSR 394
Score = 50.4 bits (115), Expect = 2e-07
Identities = 18/44 (40%), Positives = 31/44 (70%)
Frame = +2
Query: 509 ISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 640
+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 395 LDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 81.8 bits (193), Expect = 8e-17
Identities = 35/86 (40%), Positives = 53/86 (61%)
Frame = +1
Query: 1 CFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGF 180
C R L + P+Y +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +
Sbjct: 213 CRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTY 272
Query: 181 APLTSRGSRQYRALTVPELTQQMFEP 258
AP+ S + + +V E+T Q FEP
Sbjct: 273 APIVSAAKAFHESNSVQEITNQCFEP 298
Score = 62.5 bits (145), Expect = 5e-11
Identities = 29/91 (31%), Positives = 47/91 (51%), Gaps = 8/91 (8%)
Frame = +3
Query: 261 NMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIP 440
N M CDPR GRY+ ++RG + ++V + I+ K + FV+W P K +CD P
Sbjct: 300 NQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRP 359
Query: 441 PRGLK--------MAATFIGNSTAIQELFKR 509
P+ ++ A + N+T+I E + R
Sbjct: 360 PQHIEGSEIAKVDRAVCMLSNTTSIAEAWSR 390
Score = 50.4 bits (115), Expect = 2e-07
Identities = 18/44 (40%), Positives = 31/44 (70%)
Frame = +2
Query: 509 ISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 640
+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 391 LDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 62.5 bits (145), Expect = 5e-11
Identities = 29/86 (33%), Positives = 49/86 (56%), Gaps = 3/86 (3%)
Frame = +1
Query: 13 LKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT 192
L PT+ N LVS MS TT LR+PG +N DL + +++P PR HF + + P T
Sbjct: 218 LHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFT 277
Query: 193 SRGSRQYRAL---TVPELTQQMFEPR 261
++ + +A+ TV ++ +++ P+
Sbjct: 278 NQQVEEAKAIRKTTVLDVMRRLLLPK 303
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 27.9 bits (59), Expect = 1.4
Identities = 16/74 (21%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +3
Query: 315 IFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIG--NSTA 488
++ + ++ + + ++NI+ N S W+PN + +P G K++ I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 489 IQELFKRS-RNSSP 527
+EL++ + S+P
Sbjct: 403 KEELYETDLKESAP 416
>SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 684
Score = 27.5 bits (58), Expect = 1.8
Identities = 20/74 (27%), Positives = 31/74 (41%)
Frame = +2
Query: 407 QQREDRRVRHSPSWSQDGRHLHRKLHRHPGAVQAISEQFTAMFRRKAFLHWYTGEGMDEM 586
Q+ RR R W+ G + H P + +QF A RR+ +L + +
Sbjct: 287 QEISKRRGRFGNWWANSGLKAYFDAHGIPAYQPSPIDQFYAHLRRQYYLKTNGYQALKSK 346
Query: 587 EFTEAESNMNDLVS 628
+ EA SN+ D S
Sbjct: 347 AYEEA-SNVADSAS 359
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.2 bits (55), Expect = 4.2
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = -3
Query: 400 HSTKYEEFLFWMLSICSSTSFMDMRPRKMAATV 302
+ST +E+ + W++++ SST MRP + ATV
Sbjct: 199 NSTVFEDIMSWVVAMSSST----MRPIRHTATV 227
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1379
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +2
Query: 434 HSPSWSQDGRHLHRKLHRHPGAVQAISEQFTAMFRRKAF 550
H PS + HRK+ H A+ A SE+ T ++
Sbjct: 945 HIPSHNSFSDIHHRKIAAHAAAITAASEKLTPSIEETSY 983
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +3
Query: 294 RYLTVAAIFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 419
RYLT + + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
Alp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/63 (26%), Positives = 26/63 (41%), Gaps = 2/63 (3%)
Frame = -1
Query: 258 WLEHLLRELGYSQGTVLSASTGCEGSETWHEEV--ETREGNHVYGQFSEISVQLTGEPKA 85
WL H++ G +GT +G+E W+E + + GN SE + G K
Sbjct: 501 WLMHIVSISGLEEGT------SSQGNEVWNESITKQADVGNETRNFESEHNRSTQGTSKV 554
Query: 84 SGD 76
D
Sbjct: 555 GSD 557
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 7.3
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +3
Query: 294 RYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 410
+Y+ ++F G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 948
Score = 25.4 bits (53), Expect = 7.3
Identities = 10/29 (34%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 444 RGLKMAATFIGNSTA-IQELFKRSRNSSP 527
+G ++ ++G S A ++ +F+++RNSSP
Sbjct: 720 KGPELLNMYVGESEANVRNVFEKARNSSP 748
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 25.0 bits (52), Expect = 9.6
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 515 EQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 646
E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1717 ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.0 bits (52), Expect = 9.6
Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = +2
Query: 2 ASALSNCPHPPTVTLTT*FPSQCLVSPLALGSPVS*TLISENW--P*TWFPS---RVSTS 166
A +LS CP PT L++ + +V + G + S NW +W PS RV+ +
Sbjct: 263 ALSLSWCPEDPTFLLSSGKDGRAMVWNVETGESLGSFPRSGNWYTKSSWCPSNSNRVAVA 322
Query: 167 SCQVSLPSHPVEADST 214
S + + +++ +T
Sbjct: 323 SLEGKVSIFSIQSTNT 338
>SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 886
Score = 25.0 bits (52), Expect = 9.6
Identities = 24/106 (22%), Positives = 48/106 (45%), Gaps = 3/106 (2%)
Frame = -2
Query: 311 SDGEVAAVARVTGGHHVLGSNICCVSSGTVKARY--CLLPRDVRGAKPGMKKWRRGKGTM 138
++G++ + G +G +I + G + + CL D+ +PG R +
Sbjct: 28 TEGDLIECLSIGDGKWWIGRHINTNTQGIFPSNFVHCL---DIPTVRPGSSMSRTSASSF 84
Query: 137 FTANFLRSAFN*P-GNLRQVVTPDIVRETKWLRSP*VGVDSLRVRK 3
++ +S+ + P + Q +TPD+V + L P DSL+ +K
Sbjct: 85 RYSSPQKSSIDTPITSSDQGLTPDLVGSSNALNKPTRESDSLKHQK 130
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,627,538
Number of Sequences: 5004
Number of extensions: 50910
Number of successful extensions: 198
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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