Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0792X.Seq
(555 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g46500.2 68415.m05791 phosphatidylinositol 3- and 4-kinase fa... 48 6e-06
At2g46500.1 68415.m05790 phosphatidylinositol 3- and 4-kinase fa... 48 6e-06
At1g26270.1 68414.m03205 phosphatidylinositol 3- and 4-kinase fa... 45 4e-05
At5g24240.1 68418.m02852 phosphatidylinositol 3- and 4-kinase fa... 42 2e-04
At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase fa... 40 0.001
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 38 0.005
At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase fa... 37 0.008
At2g40850.1 68415.m05043 phosphatidylinositol 3- and 4-kinase fa... 37 0.010
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 31 0.39
At4g37580.1 68417.m05319 N-acetyltransferase, putative / hookles... 31 0.52
At2g23060.1 68415.m02749 GCN5-related N-acetyltransferase (GNAT)... 31 0.52
At5g15910.1 68418.m01861 dehydrogenase-related low similarity to... 30 1.2
At1g32120.1 68414.m03952 expressed protein contains Pfam profile... 29 2.8
At1g48950.1 68414.m05485 expressed protein 28 3.6
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 27 6.4
At5g01015.1 68418.m00003 expressed protein 27 6.4
At3g18773.1 68416.m02383 zinc finger (C3HC4-type RING finger) fa... 27 6.4
At5g50780.1 68418.m06291 ATP-binding region, ATPase-like domain-... 27 8.4
At5g45290.1 68418.m05560 zinc finger (C3HC4-type RING finger) fa... 27 8.4
At5g10730.1 68418.m01243 expressed protein 27 8.4
At3g08670.1 68416.m01007 expressed protein 27 8.4
>At2g46500.2 68415.m05791 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 566
Score = 47.6 bits (108), Expect = 6e-06
Identities = 21/54 (38%), Positives = 33/54 (61%), Gaps = 1/54 (1%)
Frame = +2
Query: 326 ELVLQAETAIDNGIFPERIYQGSSGSYFVK-KAGGKIIGVFKPKDEEPYGRLNP 484
+++ A + +G P R +G+ G+YF++ +G K +GVFKP DEEP NP
Sbjct: 243 DMIQSASDGLKSGNSPVRSSEGTGGAYFMQGPSGNKFVGVFKPIDEEPMAENNP 296
>At2g46500.1 68415.m05790 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 566
Score = 47.6 bits (108), Expect = 6e-06
Identities = 21/54 (38%), Positives = 33/54 (61%), Gaps = 1/54 (1%)
Frame = +2
Query: 326 ELVLQAETAIDNGIFPERIYQGSSGSYFVK-KAGGKIIGVFKPKDEEPYGRLNP 484
+++ A + +G P R +G+ G+YF++ +G K +GVFKP DEEP NP
Sbjct: 243 DMIQSASDGLKSGNSPVRSSEGTGGAYFMQGPSGNKFVGVFKPIDEEPMAENNP 296
>At1g26270.1 68414.m03205 phosphatidylinositol 3- and 4-kinase
family protein similar to phosphatidylinositol 4-kinase
type-II beta [Homo sapiens] GI:20159767; contains Pfam
profile PF00454: Phosphatidylinositol 3- and 4-kinase
Length = 630
Score = 44.8 bits (101), Expect = 4e-05
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +2
Query: 350 AIDNGIFPERIYQGSSGSYFVKKAGGKIIGVFKPKDEEPYGRLNP 484
A+ GI P + G G+Y+ K + G+ + + KP DEEPY NP
Sbjct: 158 AVKKGIDPVAVNSGLGGAYYFKNSRGESVAIVKPTDEEPYAPNNP 202
>At5g24240.1 68418.m02852 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain, Pfam profile
PF00454: Phosphatidylinositol 3- and 4-kinase
Length = 574
Score = 42.3 bits (95), Expect = 2e-04
Identities = 22/54 (40%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 326 ELVLQAETAIDNGIFPERIYQGSSGSYFVKKAGG-KIIGVFKPKDEEPYGRLNP 484
EL+ ++ G P R GS G+YF++ G K + VFKP DEEP NP
Sbjct: 245 ELISSTLEGLEKGNGPIRSSDGSGGAYFMQDPSGHKYVSVFKPIDEEPMAVNNP 298
>At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 650
Score = 39.9 bits (89), Expect = 0.001
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +2
Query: 350 AIDNGIFPERIYQGSSGSYFVKKAGGKIIGVFKPKDEEPYGRLNP 484
A+ G+ P ++ G G+Y+ + G+ + + KP DEEP+ NP
Sbjct: 162 AMKMGVEPLPVHSGLGGAYYFRNKRGESVAIVKPTDEEPFAPNNP 206
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 37.9 bits (84), Expect = 0.005
Identities = 21/57 (36%), Positives = 27/57 (47%)
Frame = +2
Query: 314 PQFSELVLQAETAIDNGIFPERIYQGSSGSYFVKKAGGKIIGVFKPKDEEPYGRLNP 484
P LV + AI +G P + G G+Y ++ G I V KP DEEP NP
Sbjct: 85 PTVRSLVAEVTIAIVSGAQPLLLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNP 141
>At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 622
Score = 37.1 bits (82), Expect = 0.008
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2
Query: 350 AIDNGIFPERIYQGSSGSYFVKKAGGKIIGVFKPKDEEPYGRLNP 484
A+ G+ P + G G+Y+ + G+ + + KP DEEP+ NP
Sbjct: 154 AMKMGVEPIPVNGGLGGAYYFRDEKGQSVAIVKPTDEEPFAPNNP 198
>At2g40850.1 68415.m05043 phosphatidylinositol 3- and 4-kinase
family protein contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 561
Score = 36.7 bits (81), Expect = 0.010
Identities = 20/57 (35%), Positives = 27/57 (47%)
Frame = +2
Query: 314 PQFSELVLQAETAIDNGIFPERIYQGSSGSYFVKKAGGKIIGVFKPKDEEPYGRLNP 484
P LV + A+ +G P + G G+Y ++ G I V KP DEEP NP
Sbjct: 105 PTVRALVAEVTMAMVSGAQPLLLPSGMGGAYLLQTGKGHNIAVAKPVDEEPLAFNNP 161
>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 340
Score = 31.5 bits (68), Expect = 0.39
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = -3
Query: 232 ESRLPSNSTAGTKQISSELLDNETTPSSGGAVVVFG 125
E LPSN + SEL+D +T PSS A G
Sbjct: 123 EKNLPSNMLPQIYDVLSELVDRKTLPSSSSAAAAVG 158
>At4g37580.1 68417.m05319 N-acetyltransferase, putative / hookless1
(HLS1) contains Pfam profile PF00583: acetyltransferase,
GNAT family; identical to cDNA putative
N-acetyltransferase hookless1 (HLS1) GI:1277089
Length = 403
Score = 31.1 bits (67), Expect = 0.52
Identities = 20/61 (32%), Positives = 32/61 (52%)
Frame = -3
Query: 520 TGSILVHPFHPXGVQTTVGLFVFRLENADDFSARFFYEVRPAGSLVYPLREDAVINGRLR 341
T SILV+P + V + + V +LE D A Y +R + + +P D+V+N +L
Sbjct: 163 TPSILVNPVYAHRVNVSRRVTVIKLEPVD---AETLYRIRFSTTEFFPRDIDSVLNNKLS 219
Query: 340 L 338
L
Sbjct: 220 L 220
>At2g23060.1 68415.m02749 GCN5-related N-acetyltransferase (GNAT)
family protein contains Pfam profile PF00583:
acetyltransferase, GNAT family
Length = 413
Score = 31.1 bits (67), Expect = 0.52
Identities = 20/61 (32%), Positives = 33/61 (54%)
Frame = -3
Query: 520 TGSILVHPFHPXGVQTTVGLFVFRLENADDFSARFFYEVRPAGSLVYPLREDAVINGRLR 341
T SILV+P + V + + V +LE +D A Y +R + + +P D+V+N +L
Sbjct: 173 TPSILVNPVYAHRVNISRRVTVIKLEPSD---AELLYRLRFSTTEFFPRDIDSVLNNKLS 229
Query: 340 L 338
L
Sbjct: 230 L 230
>At5g15910.1 68418.m01861 dehydrogenase-related low similarity to
SP|Q9R1J0 NAD(P)-dependent steroid dehydrogenase (EC
1.1.1.-) {Mus musculus}
Length = 269
Score = 29.9 bits (64), Expect = 1.2
Identities = 17/48 (35%), Positives = 24/48 (50%)
Frame = -3
Query: 325 AELGVIWEVIEGYFEVAAAAKQRLDSRFTPGESRLPSNSTAGTKQISS 182
A+ GVI +I GYFE A + + +F S L GT+Q+ S
Sbjct: 152 ADFGVINNLIRGYFEGKRATEAEILDKFGNRGSVLRPGFIHGTRQVGS 199
>At1g32120.1 68414.m03952 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 1206
Score = 28.7 bits (61), Expect = 2.8
Identities = 22/76 (28%), Positives = 35/76 (46%)
Frame = +3
Query: 240 VNRESSRCLAAAATSKYPSITSQMTPSSASWYYRRRRPLITASSRRGYTKDPAGRTS*KK 419
+N + + SK P ++S ++ SS+S + R A+SR GY P+ R S +
Sbjct: 758 INSRGQKNPPSPRVSKEPYMSSSLSVSSSSTRKKPPRSHEAANSR-GYNHTPSLRASKEP 816
Query: 420 RAEKSSAFSSRKTKSP 467
S + SS K P
Sbjct: 817 YKSSSLSGSSSTRKKP 832
Score = 27.1 bits (57), Expect = 8.4
Identities = 23/65 (35%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = +3
Query: 282 SKYPSITSQMTPSSASWYYRRRRPLI-TASSRRGYTKDPAGRTS*K--KRAEKSSAFSSR 452
SK P +S ++ SS++ R++ P ASS RGY P+ R S + K S + S+
Sbjct: 813 SKEPYKSSSLSGSSST---RKKPPRSHEASSSRGYNHPPSPRVSKELNKTPSISGSPSAT 869
Query: 453 KTKSP 467
+ KSP
Sbjct: 870 RNKSP 874
>At1g48950.1 68414.m05485 expressed protein
Length = 608
Score = 28.3 bits (60), Expect = 3.6
Identities = 12/26 (46%), Positives = 19/26 (73%)
Frame = -3
Query: 262 QRLDSRFTPGESRLPSNSTAGTKQIS 185
Q +D FTP +S+LPS+ST+ K ++
Sbjct: 11 QIMDKLFTPSKSQLPSSSTSVDKLLN 36
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 27.5 bits (58), Expect = 6.4
Identities = 18/47 (38%), Positives = 24/47 (51%)
Frame = +3
Query: 156 GVVSLSSSSEEICLVPAVEFEGNLDSPGVNRESSRCLAAAATSKYPS 296
G+V SSSS + P ++ GNL +N E L AA SK+ S
Sbjct: 373 GLVKTSSSSSKDEPKPYIDGTGNLQVWRINCEEKILLEAAEQSKFYS 419
>At5g01015.1 68418.m00003 expressed protein
Length = 104
Score = 27.5 bits (58), Expect = 6.4
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +1
Query: 517 PCCFGRSCLIPNQ 555
PCCFG +C +PN+
Sbjct: 73 PCCFGINCQLPNK 85
>At3g18773.1 68416.m02383 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 220
Score = 27.5 bits (58), Expect = 6.4
Identities = 12/48 (25%), Positives = 23/48 (47%)
Frame = +3
Query: 126 PNTTTAPPDDGVVSLSSSSEEICLVPAVEFEGNLDSPGVNRESSRCLA 269
P + + P D V+ + + ++P V + ++ PGV E CL+
Sbjct: 88 PISIPSTPRDSSVNKGIKKKALKMLPVVNYSPEINLPGVGEECVICLS 135
>At5g50780.1 68418.m06291 ATP-binding region, ATPase-like
domain-containing protein low similarity to microrchidia
[Homo sapiens] GI:5410257; contains Pfam profile
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein
Length = 823
Score = 27.1 bits (57), Expect = 8.4
Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 9/56 (16%)
Frame = +3
Query: 138 TAPPDDGVVSLSSSSE---------EICLVPAVEFEGNLDSPGVNRESSRCLAAAA 278
T PP + V+ LSSS+E EI + +V+ G D G R S +A+ A
Sbjct: 27 TIPPPESVIELSSSNEGSELGENLDEIAEIQSVDRTGGDDVSGTKRARSDSIASPA 82
>At5g45290.1 68418.m05560 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 545
Score = 27.1 bits (57), Expect = 8.4
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3
Query: 252 SSRCLAAAATSKYPSITSQMTPSSASWYYRRRR 350
S CL ++A ++ S +Q PSS+S RRRR
Sbjct: 6 SKSCLGSSAMAREASDVAQPPPSSSSGGGRRRR 38
>At5g10730.1 68418.m01243 expressed protein
Length = 287
Score = 27.1 bits (57), Expect = 8.4
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = -3
Query: 325 AELGVIWEVIEGYFEVAAAAKQRLDSRFTPGESRLPSNSTAGTKQISS 182
A+ G+ ++ GY+E AA+ L +RF G L GT+ + S
Sbjct: 167 ADFGLANYLLRGYYEGKRAAETELLTRFAYGGIILRPGFIYGTRSVGS 214
>At3g08670.1 68416.m01007 expressed protein
Length = 567
Score = 27.1 bits (57), Expect = 8.4
Identities = 18/65 (27%), Positives = 31/65 (47%)
Frame = +3
Query: 129 NTTTAPPDDGVVSLSSSSEEICLVPAVEFEGNLDSPGVNRESSRCLAAAATSKYPSITSQ 308
+++ A P + +SS+ + + + E S R SS + +TS+Y S TS
Sbjct: 114 HSSLAAPKIASSARASSASKASRLSVSQSESGYHSSRPARSSSVTRPSISTSQYSSFTSG 173
Query: 309 MTPSS 323
+PSS
Sbjct: 174 RSPSS 178
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,311,598
Number of Sequences: 28952
Number of extensions: 252059
Number of successful extensions: 854
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 853
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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