Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0788.Seq
(717 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 45 4e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 44 1e-04
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 44 1e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 44 1e-04
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 43 2e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 42 4e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 40 0.002
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 35 0.062
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 33 0.25
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 31 1.0
At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein ... 30 1.3
At3g30380.1 68416.m03835 expressed protein ; expression supporte... 29 4.1
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 7.1
At5g50630.1 68418.m06272 nodulin family protein similar to nodul... 27 9.4
At5g50520.1 68418.m06257 nodulin family protein similar to nodul... 27 9.4
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 9.4
At1g05200.1 68414.m00524 glutamate receptor family protein (GLR3... 27 9.4
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 45.2 bits (102), Expect = 4e-05
Identities = 26/92 (28%), Positives = 51/92 (55%), Gaps = 8/92 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 412
D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN +
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 508
+++ + +GVL+V P+ P +++ I+
Sbjct: 126 EIKASM-ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 44.0 bits (99), Expect = 1e-04
Identities = 27/92 (29%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 412
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 508
V++ + +GVLTV+ P+ P +++ I+
Sbjct: 128 EVKATM-ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 44.0 bits (99), Expect = 1e-04
Identities = 30/91 (32%), Positives = 47/91 (51%), Gaps = 8/91 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 412
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPI 505
V++ + +GVLTV P+ A K + I
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQVKSI 153
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 43.6 bits (98), Expect = 1e-04
Identities = 28/92 (30%), Positives = 50/92 (54%), Gaps = 8/92 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 412
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 508
V++ + +GVLTV P+ + +++ I+
Sbjct: 122 QVKAAM-ENGVLTVTVPKAETKKADVKSIQIS 152
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 42.7 bits (96), Expect = 2e-04
Identities = 19/81 (23%), Positives = 41/81 (50%), Gaps = 3/81 (3%)
Frame = +2
Query: 251 QLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNPDTVE 421
+ D+ S E++ + D ++++G+ ++ + + R + R LP+NC D ++
Sbjct: 141 RFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIK 200
Query: 422 SRLSSDGVLTVIAPRTPAATK 484
+ L +GVL + P+T K
Sbjct: 201 AEL-KNGVLFITIPKTKVERK 220
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 41.9 bits (94), Expect = 4e-04
Identities = 27/92 (29%), Positives = 49/92 (53%), Gaps = 8/92 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 412
DV EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN +
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPIT 508
V++ + +GVL+V P+ + ++V I+
Sbjct: 125 EVKASM-ENGVLSVTVPKVQESKPEVKSVDIS 155
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 39.9 bits (89), Expect = 0.002
Identities = 28/91 (30%), Positives = 45/91 (49%), Gaps = 8/91 (8%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPD 412
D+ EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMD 121
Query: 413 TVESRLSSDGVLTVIAPRTPAATKNERAVPI 505
V++ + +GVLTV P+ K + I
Sbjct: 122 QVKASM-ENGVLTVTVPKVETNKKKAQVKSI 151
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 34.7 bits (76), Expect = 0.062
Identities = 20/92 (21%), Positives = 46/92 (50%), Gaps = 9/92 (9%)
Frame = +2
Query: 257 DVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYALPENCNP 409
+V + E++ + DG + ++G H+ ++ E Y S + + +LP++
Sbjct: 141 EVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDAKV 200
Query: 410 DTVESRLSSDGVLTVIAPRTPAATKNERAVPI 505
+ +++ L + GVL ++ PRT KN + + +
Sbjct: 201 EDIKAELKN-GVLNLVIPRTEKPKKNVQEISV 231
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 32.7 bits (71), Expect = 0.25
Identities = 27/118 (22%), Positives = 44/118 (37%)
Frame = +2
Query: 278 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 457
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 458 APRTPAATKNERAVPITKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSVIACVNFI 631
R KN +PI+ + R + + N +R+ + KS+ +F+
Sbjct: 108 DDRHSRMLKNVTELPISAFQGESKNKRVVFTEPYPESEFNPTRDVLEGKSLTTIDDFM 165
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 30.7 bits (66), Expect = 1.0
Identities = 19/71 (26%), Positives = 32/71 (45%)
Frame = +2
Query: 254 LDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLS 433
+D+ EEI V+ D ++ + F R++ LPE+ + + +
Sbjct: 41 VDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIGISAGYE 100
Query: 434 SDGVLTVIAPR 466
DGVLTVI P+
Sbjct: 101 -DGVLTVIVPK 110
>At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 675
Score = 30.3 bits (65), Expect = 1.3
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +2
Query: 461 PRTPAATKNERAVP---ITKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSV 610
P+ PA++KN + P I S +L + + + NND AVN+ +V
Sbjct: 594 PQKPASSKNLKTTPSSHIQSSLLSAMKLAAEFESAKVERGNNDPTEAVNKSNV 646
>At3g30380.1 68416.m03835 expressed protein ; expression supported
by MPSS
Length = 399
Score = 28.7 bits (61), Expect = 4.1
Identities = 14/38 (36%), Positives = 23/38 (60%)
Frame = +2
Query: 497 VPITKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSV 610
VP+T+ ARS +R P R +T Q + SR + +Q+ +
Sbjct: 271 VPLTEKARSSTDIREPARP--STDQRDKSRTSTDQREM 306
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 7.1
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +2
Query: 101 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 214
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At5g50630.1 68418.m06272 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +1
Query: 511 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 681
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At5g50520.1 68418.m06257 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +1
Query: 511 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 681
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 9.4
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +2
Query: 278 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 454
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
>At1g05200.1 68414.m00524 glutamate receptor family protein (GLR3.4)
plant glutamate receptor family, PMID:11379626
Length = 959
Score = 27.5 bits (58), Expect = 9.4
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 470 PAATKNERAVPITKPARSGRRLRSP 544
P + ERA +++P+RSGR R+P
Sbjct: 889 PESADEERAGEVSEPSRSGRGSRAP 913
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,762,847
Number of Sequences: 28952
Number of extensions: 271839
Number of successful extensions: 872
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 842
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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