BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0786.Seq (748 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex ... 93 3e-20 SPAC6F12.14 |cut23|apc8|anaphase-promoting complex subunit Apc8 ... 60 2e-10 SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 37 0.004 SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 30 0.30 SPBC11G11.01 |fis1||mitochondrial fission protein Fis1 |Schizosa... 30 0.40 SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosacch... 27 2.8 SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha... 27 2.8 SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 27 3.8 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 5.0 SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomy... 26 5.0 SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 23 6.5 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 8.7 SPBC725.07 |pex5||peroxisomal targeting signal receptor |Schizos... 25 8.7 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 25 8.7 >SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex subunit Apc3|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 93.5 bits (222), Expect = 3e-20 Identities = 41/84 (48%), Positives = 55/84 (65%) Frame = +1 Query: 259 MEIYSTCLWHLQREAQLSALAQELVELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQ 438 ME++ST LWHLQ+ LS LA E +E N +P +W + NCFSL +E ALK RA+Q Sbjct: 400 MEVFSTALWHLQKSVPLSYLAHETLETNPYSPESWCILANCFSLQREHSQALKCINRAIQ 459 Query: 439 LNPDAAYAHALLGHEYAVAEETDK 510 L+P YA+ L GHE++ EE +K Sbjct: 460 LDPTFEYAYTLQGHEHSANEEYEK 483 Score = 48.0 bits (109), Expect = 1e-06 Identities = 22/73 (30%), Positives = 41/73 (56%) Frame = +2 Query: 38 LLSLMKEIGEAYKYLAFLDCKNAIKSFQELPPKQLASPWVQTMIARSHYELAQYEAAAKI 217 L+ L+K G+ LA + A+ FQ LP +Q +P+V + +++EL YE + ++ Sbjct: 326 LMELLKLFGKGVYLLAQYKLREALNCFQSLPIEQQNTPFVLAKLGITYFELVDYEKSEEV 385 Query: 218 FSEIRKQYPNRTE 256 F ++R P+R + Sbjct: 386 FQKLRDLSPSRVK 398 Score = 43.2 bits (97), Expect = 4e-05 Identities = 20/71 (28%), Positives = 38/71 (53%) Frame = +3 Query: 534 VSIDPRNYVAWFGIATVYARQERWKASEVHIRRALSIHPLSGVLRCQLGLAQSALGKMER 713 + ++ R+Y AW+G+ VY + R ++ H +RA I+P + VL +G+ ++ Sbjct: 492 IRVNVRHYNAWYGLGMVYLKTGRNDQADFHFQRAAEINPNNSVLITCIGMIYERCKDYKK 551 Query: 714 ALSTLEKAVAL 746 AL ++A L Sbjct: 552 ALDFYDRACKL 562 >SPAC6F12.14 |cut23|apc8|anaphase-promoting complex subunit Apc8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 565 Score = 60.5 bits (140), Expect = 2e-10 Identities = 30/82 (36%), Positives = 48/82 (58%) Frame = +1 Query: 259 MEIYSTCLWHLQREAQLSALAQELVELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQ 438 M+ YS L+ L+ +++L LAQ +++ P + GN +SL E E A+ +F+RA+Q Sbjct: 307 MDTYSNVLFVLENKSKLGFLAQVASSIDKFRPETCSIIGNYYSLLSEHEKAVTYFKRALQ 366 Query: 439 LNPDAAYAHALLGHEYAVAEET 504 LN + A L+GHEY + T Sbjct: 367 LNRNYLSAWTLMGHEYVELKNT 388 Score = 28.3 bits (60), Expect = 1.2 Identities = 17/56 (30%), Positives = 27/56 (48%) Frame = +1 Query: 331 VELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQLNPDAAYAHALLGHEYAVAE 498 ++LNR AW + G+ + K A++ +R AV +N A LG Y V + Sbjct: 365 LQLNRNYLSAWTLMGHEYVELKNTHAAIESYRLAVDVNRKDYRAWYGLGQTYEVLD 420 Score = 26.6 bits (56), Expect = 3.8 Identities = 16/52 (30%), Positives = 24/52 (46%) Frame = +1 Query: 331 VELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQLNPDAAYAHALLGHEY 486 V++NR + AW G + + AL +F+RA L P LG+ Y Sbjct: 399 VDVNRKDYRAWYGLGQTYEVLDMHFYALYYFQRATALRPYDQRMWQALGNCY 450 >SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1389 Score = 36.7 bits (81), Expect = 0.004 Identities = 19/69 (27%), Positives = 36/69 (52%) Frame = +3 Query: 540 IDPRNYVAWFGIATVYARQERWKASEVHIRRALSIHPLSGVLRCQLGLAQSALGKMERAL 719 I P++ AW G+ YAR R+ ++ RA + P ++ + + +G+ E A+ Sbjct: 679 ISPKDTNAWSGLGEAYARSGRYVSALKAFNRASILDPDDWYVKYFIATLEKDMGEYEVAV 738 Query: 720 STLEKAVAL 746 STL + +A+ Sbjct: 739 STLSEILAV 747 Score = 28.7 bits (61), Expect = 0.93 Identities = 19/69 (27%), Positives = 31/69 (44%) Frame = +3 Query: 534 VSIDPRNYVAWFGIATVYARQERWKASEVHIRRALSIHPLSGVLRCQLGLAQSALGKMER 713 ++ DPRNY A G V E++ + + L H L +L G + + Sbjct: 457 LAADPRNYHALLGKGLVQIENEQYSDAVKTLGLLLDDHENDPSL-SELSWCYFKTGNLPK 515 Query: 714 ALSTLEKAV 740 A+ST+EK + Sbjct: 516 AISTVEKCL 524 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/46 (30%), Positives = 22/46 (47%) Frame = +1 Query: 322 QELVELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQLNPDAAY 459 Q + ++ + AW G ++ +ALK F RA L+PD Y Sbjct: 674 QSALRISPKDTNAWSGLGEAYARSGRYVSALKAFNRASILDPDDWY 719 >SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosaccharomyces pombe|chr 2|||Manual Length = 2358 Score = 30.3 bits (65), Expect = 0.30 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%) Frame = +2 Query: 179 HYELAQYEAAA-KIFSEIRKQYPNRTEGWK 265 H++ A+ E KIF+ IR+QYP GWK Sbjct: 551 HFDFAKPELKMFKIFNFIREQYPALKSGWK 580 >SPBC11G11.01 |fis1||mitochondrial fission protein Fis1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 29.9 bits (64), Expect = 0.40 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = +2 Query: 167 IARSHYELAQYEAAAKIFSEIRKQYPNRTEGWKFTVPVY 283 IA SHY+L QYE + + + + + PN E K +Y Sbjct: 84 IALSHYKLKQYEESRRYLNMLLSKDPNSPEALKLKNRLY 122 >SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 476 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/41 (31%), Positives = 21/41 (51%) Frame = +2 Query: 11 PPKNSNGVALLSLMKEIGEAYKYLAFLDCKNAIKSFQELPP 133 P KN+ + + KE+GEAY L+ + + S +L P Sbjct: 378 PDKNAGNLEAEARFKEVGEAYTILSDPESRRRFDSGVDLEP 418 >SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccharomyces pombe|chr 2|||Manual Length = 1102 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 322 QELVELNRTNPIAWLVAGNCFSLHKERETALKFFRRAVQLNP 447 Q+ V + NP W G + + + AL + RA++LNP Sbjct: 608 QQAVYRDGRNPTFWCSIGVLYYQINQYQDALDAYSRAIRLNP 649 >SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = +3 Query: 534 VSIDPRNYVAWFGIATVYARQERWKASEVHIRRALSIHPLSG 659 + I+P +Y WF +++ A+ R LSI+P G Sbjct: 548 LKINPLSYPTWFTYGCAALELQKYDAAMEAFSRCLSINPEDG 589 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.2 bits (55), Expect = 5.0 Identities = 14/40 (35%), Positives = 20/40 (50%) Frame = -3 Query: 194 EPIHNGYVRSWFVPKVKPIVLEVTLERILLHFYSLKMQDI 75 +P+H G + W V K+K VLE E + S K D+ Sbjct: 1553 KPMHYGIISEWDVSKLKIRVLEQGRESKKVSVASYKFGDL 1592 >SPBC16E9.11c |pub3||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 786 Score = 26.2 bits (55), Expect = 5.0 Identities = 13/43 (30%), Positives = 22/43 (51%) Frame = +2 Query: 581 SLRTTRTLESIRSSHTPCSQYTSTFRSVEMSVRSGTVCSRKNG 709 S TT T + R++ P + ++ +S S R+GT + NG Sbjct: 151 STPTTTTARTTRTTPRPTATTNTSNQSTSNSTRNGTSAATSNG 193 >SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 475 Score = 22.6 bits (46), Expect(2) = 6.5 Identities = 8/22 (36%), Positives = 14/22 (63%) Frame = +2 Query: 134 KQLASPWVQTMIARSHYELAQY 199 K+ SPW+ +R +E+A+Y Sbjct: 406 KETWSPWILPSESRLKFEMAKY 427 Score = 21.4 bits (43), Expect(2) = 6.5 Identities = 11/18 (61%), Positives = 11/18 (61%) Frame = +2 Query: 65 EAYKYLAFLDCKNAIKSF 118 E Y LAFL N IKSF Sbjct: 355 EGYVPLAFLASFNRIKSF 372 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 25.4 bits (53), Expect = 8.7 Identities = 12/48 (25%), Positives = 22/48 (45%) Frame = -1 Query: 535 TANLKLARAYQFLLLRRIRALIMHVHKQHLDLAELHV*RTLMQFHVPY 392 T N+ L + + +MHV + ++ LH+ R+ H+PY Sbjct: 1077 TLNMALKSEFDMTDFNNSGSKLMHVLHARIFISVLHLWRSADDLHLPY 1124 >SPBC725.07 |pex5||peroxisomal targeting signal receptor |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 8.7 Identities = 15/66 (22%), Positives = 27/66 (40%) Frame = +3 Query: 543 DPRNYVAWFGIATVYARQERWKASEVHIRRALSIHPLSGVLRCQLGLAQSALGKMERALS 722 +P N + W + E+ + RA+S+ P +R + ++ LG E A Sbjct: 468 EPSNEILWNKLGAALTNAEKNTEAVSSYNRAVSLQPQYVRVRSNMAVSNINLGYFEDAAK 527 Query: 723 TLEKAV 740 L A+ Sbjct: 528 HLLAAI 533 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 25.4 bits (53), Expect = 8.7 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 3/65 (4%) Frame = -3 Query: 197 IEPIHNGY---VRSWFVPKVKPIVLEVTLERILLHFYSLKMQDIYKLLLFLS*DSTMLLH 27 I+ IH G + + FV PI + R +LH +S + +DI L L S+ +H Sbjct: 732 IKSIHPGSLSGISNSFVNSKHPIEQWQSRLRKILHLFSGRNEDIASLQCVLQFGSSERIH 791 Query: 26 LNSLE 12 L ++ Sbjct: 792 LEDIQ 796 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,935,217 Number of Sequences: 5004 Number of extensions: 56575 Number of successful extensions: 183 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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