BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0774.Seq (715 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1734.10c |||mRNA processing protein |Schizosaccharomyces pom... 27 2.0 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 2.7 SPBC2G2.03c |sbh1||translocon beta subunit Sbh1|Schizosaccharomy... 27 2.7 SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 3.5 >SPBC1734.10c |||mRNA processing protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 332 Score = 27.5 bits (58), Expect = 2.0 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -1 Query: 169 RNESCGGPLQSGPGFTRSCSFCNS 98 R +S G + GP F CSFCN+ Sbjct: 153 RLQSLEGKVAYGPNFKLHCSFCNA 176 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 27.1 bits (57), Expect = 2.7 Identities = 15/45 (33%), Positives = 27/45 (60%) Frame = +3 Query: 552 QRSSCPSTKHKTVAPFVKKYIVYFSKSFQTQQKFPSLFSIASTSP 686 QR S P T+ + +++K + + +Q Q+K S+FS+A T+P Sbjct: 1150 QRISNPVTRFP-LPDWLQKRVAVLNSKYQ-QKKIDSIFSLAPTNP 1192 >SPBC2G2.03c |sbh1||translocon beta subunit Sbh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 102 Score = 27.1 bits (57), Expect = 2.7 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 135 GPGSHVVVAFVIRETTRRPQAGPAGARSA 49 GP S + + + T+ +GPAGAR+A Sbjct: 20 GPKSQIRRRAAVEKNTKESNSGPAGARAA 48 >SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 26.6 bits (56), Expect = 3.5 Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 7/65 (10%) Frame = -3 Query: 650 FLLCLKRFRKINNIFFYKWCYC----FVFSR---WTA*TLLLPLYLRVFNNIHKVVVLNL 492 F++ F ++ +FF+ C C F+FS + L LPL LRVF+ + ++ L Sbjct: 143 FIVYFSSFPILHFLFFFFLCVCVFLSFLFSLSHLLSLAILFLPLLLRVFSTLSRLPRLFC 202 Query: 491 FGLRK 477 L+K Sbjct: 203 LCLQK 207 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,807,286 Number of Sequences: 5004 Number of extensions: 56279 Number of successful extensions: 118 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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