BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0742.Seq (603 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 28 0.91 SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 28 1.2 SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 27 1.6 SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 27 2.1 SPBC577.04 |||human THOC5 homolog|Schizosaccharomyces pombe|chr ... 25 6.4 SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S... 25 6.4 SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 25 6.4 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 25 6.4 SPBC1347.13c |||ribose methyltransferase|Schizosaccharomyces pom... 25 6.4 SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr... 25 8.5 SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 25 8.5 SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces pomb... 25 8.5 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 28.3 bits (60), Expect = 0.91 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -3 Query: 127 HGPHELLHRVEGGEVSRAGPREPSAL 50 HGP L ++GG VSR R+ SAL Sbjct: 1334 HGPTSLSSMLKGGSVSRTPSRQNSAL 1359 >SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity factor complex subunit Ctf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/34 (50%), Positives = 21/34 (61%), Gaps = 1/34 (2%) Frame = +2 Query: 293 ILPIALTTSSLP-SEGGGTRGPALTVSRSRTANL 391 I P+ +TSSLP S+GGG PA+ S S NL Sbjct: 118 IPPVLHSTSSLPASQGGGMPSPAI-YSSSMATNL 150 >SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modification enzyme|Schizosaccharomyces pombe|chr 2|||Manual Length = 666 Score = 27.5 bits (58), Expect = 1.6 Identities = 11/25 (44%), Positives = 13/25 (52%) Frame = +2 Query: 527 FLHKGGHLXWLSQQALIDCSWGFGN 601 F HK HL WL + L SW + N Sbjct: 304 FPHKARHLIWLEPEGLDPNSWWYPN 328 >SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |Schizosaccharomyces pombe|chr 1|||Manual Length = 649 Score = 27.1 bits (57), Expect = 2.1 Identities = 12/42 (28%), Positives = 19/42 (45%) Frame = +2 Query: 92 TFNPMKEFVRPVHDAHVHDEFERFKVKLQKQYASDLEHEKRL 217 TF + FV P ++ + K+K K+ S + KRL Sbjct: 263 TFGTLVSFVEPTITGSEQSDYRQIKIKKSKKKKSKSDRRKRL 304 >SPBC577.04 |||human THOC5 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 200 Score = 25.4 bits (53), Expect = 6.4 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = -1 Query: 477 HQLIFNGSDRSEQSPVVFRRQLYAQLLHSRFAVRE 373 H+L + D +Q+P +RQL ++LL R A R+ Sbjct: 24 HELKQHSRDADDQNPEQIKRQLMSKLLILREANRK 58 >SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|Schizosaccharomyces pombe|chr 3|||Manual Length = 957 Score = 25.4 bits (53), Expect = 6.4 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 3/48 (6%) Frame = +2 Query: 95 FNPMKEFVRPVHDAHVHDEFERFKVKLQK---QYASDLEHEKRLNIFR 229 F +KE +P+ D V + F +KL K Q A DL R R Sbjct: 222 FKKLKEVQQPLSDVSVPSDVLPFTIKLLKKISQRAQDLSRSHRNEFLR 269 >SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 25.4 bits (53), Expect = 6.4 Identities = 14/44 (31%), Positives = 20/44 (45%), Gaps = 1/44 (2%) Frame = -2 Query: 512 PPHVPNDQHEPNTN*SLTGVTAPN-NLQSCSGGSFTLNSSTRDL 384 P PN + PN N + G+ P L S + S + NS+ L Sbjct: 480 PQTFPNSGNNPNFNRGIAGIVPPGWRLVSSNTQSLSTNSAMTSL 523 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 25.4 bits (53), Expect = 6.4 Identities = 11/41 (26%), Positives = 20/41 (48%) Frame = -3 Query: 142 DVGVVHGPHELLHRVEGGEVSRAGPREPSALHVTVNFEDVR 20 D G +H P++ +V E+S+ P S + V D++ Sbjct: 2 DSGEMHHPYQAFSKVGKCEISQTNPSFSSGMRCLVRSADIQ 42 >SPBC1347.13c |||ribose methyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 301 Score = 25.4 bits (53), Expect = 6.4 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 125 VHDAHVHDEFERFKVKLQKQYASDLEHEKRLN 220 V +A +H EFE F KLQ + +++ LN Sbjct: 56 VQNADIHSEFEEFLNKLQYKIPIKSVNKEHLN 87 >SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 25.0 bits (52), Expect = 8.5 Identities = 23/64 (35%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Frame = -2 Query: 599 SRNPRSSR*APAG*AXTGARLCAGTARPPPPHVPNDQHEPNTN*SLTGVTAP---NNLQS 429 S P R APA A T A L T PPP Q P T ++ G ++P NL S Sbjct: 6 SAAPPPRRAAPARSASTAAALPPRTMAPPPAPSRVQQAPPPT--AVQGGSSPGFFGNLVS 63 Query: 428 CSGG 417 + G Sbjct: 64 TAAG 67 >SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 25.0 bits (52), Expect = 8.5 Identities = 13/60 (21%), Positives = 28/60 (46%) Frame = +2 Query: 74 GSRHFATFNPMKEFVRPVHDAHVHDEFERFKVKLQKQYASDLEHEKRLNIFRQSLRYIHS 253 G + + ++ + D++ E E L+K+ + EHEK + ++ L+ +HS Sbjct: 201 GRKDIVKLSELRNCINSSKDSNQIQELENL---LRKEKQRNTEHEKIIRRMKKELKELHS 257 >SPCC736.15 |||protein kinase inhibitor |Schizosaccharomyces pombe|chr 3|||Manual Length = 351 Score = 25.0 bits (52), Expect = 8.5 Identities = 12/42 (28%), Positives = 24/42 (57%) Frame = +2 Query: 155 ERFKVKLQKQYASDLEHEKRLNIFRQSLRYIHSIIERTAVSP 280 E+FK + + EH ++LN+ R++ ++I+ T V+P Sbjct: 193 EKFKQAMTFNLDALHEHAEKLNLIATYGRHLLNLIDDTPVTP 234 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,263,879 Number of Sequences: 5004 Number of extensions: 42644 Number of successful extensions: 158 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 264253462 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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