BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0735X.Seq (359 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 118 3e-28 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 67 9e-13 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 48 6e-07 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 46 2e-06 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 41 7e-05 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 37 0.001 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 34 0.006 SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 26 1.5 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 2.0 SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 26 2.0 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 2.0 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 4.7 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 24 6.2 SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 24 8.2 SPAC637.12c |mst1||histone acetyltransferase Mst1|Schizosaccharo... 24 8.2 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 118 bits (284), Expect = 3e-28 Identities = 52/63 (82%), Positives = 58/63 (92%) Frame = +3 Query: 57 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGXPRHQGVMVGMGQTDSYVGHEAQSK 236 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVG PRH G+MVGMGQ DSYVG EAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 237 TGI 245 GI Sbjct: 62 RGI 64 Score = 79.8 bits (188), Expect = 1e-16 Identities = 30/35 (85%), Positives = 32/35 (91%) Frame = +2 Query: 254 EYPIEHGIITNWDDMEKTWHHNFYNELRVAPEEHP 358 +YPIEHGI+ NWDDMEK WHH FYNELRVAPEEHP Sbjct: 68 KYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 66.9 bits (156), Expect = 9e-13 Identities = 30/61 (49%), Positives = 40/61 (65%) Frame = +3 Query: 63 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGXPRHQGVMVGMGQTDSYVGHEAQSKTG 242 D + +DNGSG KAGFAGDD P+ +FP+ VG +H+ VM Q D +VG EAQ+ G Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 243 I 245 + Sbjct: 68 L 68 Score = 44.0 bits (99), Expect = 7e-06 Identities = 18/34 (52%), Positives = 23/34 (67%), Gaps = 1/34 (2%) Frame = +2 Query: 260 PIEHGIITNWDDMEKTWHHNFYN-ELRVAPEEHP 358 PIE GII NW DME+ W + + + +L PEEHP Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHP 107 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 47.6 bits (108), Expect = 6e-07 Identities = 17/34 (50%), Positives = 22/34 (64%) Frame = +2 Query: 254 EYPIEHGIITNWDDMEKTWHHNFYNELRVAPEEH 355 +YPI HG I NWD ME+ W + + LR PE+H Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDH 124 Score = 40.7 bits (91), Expect = 7e-05 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +3 Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 158 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 45.6 bits (103), Expect = 2e-06 Identities = 25/57 (43%), Positives = 34/57 (59%), Gaps = 2/57 (3%) Frame = +3 Query: 69 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGXP--RHQGVMVGMGQTDSYVGHEAQS 233 A +V+DNG+G K G+A D+ PR FPSIVG P R + + D VG EA++ Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEA 60 Score = 37.1 bits (82), Expect = 8e-04 Identities = 11/31 (35%), Positives = 24/31 (77%) Frame = +2 Query: 254 EYPIEHGIITNWDDMEKTWHHNFYNELRVAP 346 +YP+E+GII ++++M + W + F+ +L++ P Sbjct: 68 KYPMENGIIRDFEEMNQLWDYTFFEKLKIDP 98 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 40.7 bits (91), Expect = 7e-05 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 60 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 161 D+V+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 Score = 30.7 bits (66), Expect = 0.071 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +2 Query: 263 IEHGIITNWDDMEKTWHHNFYNELRVAPEEHP 358 I +G + NWD W + +L+ P EHP Sbjct: 69 IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHP 100 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 36.7 bits (81), Expect = 0.001 Identities = 23/79 (29%), Positives = 43/79 (54%) Frame = +3 Query: 6 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGXPRHQGVM 185 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R + Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR--- 60 Query: 186 VGMGQTDSYVGHEAQSKTG 242 + +T + VG++ + G Sbjct: 61 -KLSRTSTLVGNDTLIEVG 78 Score = 29.5 bits (63), Expect = 0.16 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +2 Query: 260 PIEHGIITNWDDMEKTWHHNFYNELRVAPEEHP 358 P E +I+NWD ME+ + F +L + EHP Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHP 117 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 34.3 bits (75), Expect = 0.006 Identities = 12/34 (35%), Positives = 23/34 (67%) Frame = +3 Query: 60 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 161 +++ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 Score = 27.1 bits (57), Expect = 0.88 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +2 Query: 269 HGIITNWDDMEKTWHHNFYNELRVAPEEH 355 +GII +W+ TW +L+V P E+ Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEY 102 >SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 685 Score = 26.2 bits (55), Expect = 1.5 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 2/51 (3%) Frame = -1 Query: 356 GAPRGLH-AAHCR-SCGARFSPCHPSW**YRARWGTQSEDACLALGFVTHV 210 GAP GL AAH S +F+P HP W R R+ + AC+ + H+ Sbjct: 34 GAPMGLAPAAHVLFSRIMKFNPAHPKW-LNRDRFILSNGHACVLQYIMCHL 83 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 25.8 bits (54), Expect = 2.0 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = +3 Query: 120 GDDAPRAVFPSIVGXPRHQGVMVGMGQTDSYVGHEAQSKTG 242 G+ PRA F ++ P H G+++ M D G E S G Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKG 540 >SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 25.8 bits (54), Expect = 2.0 Identities = 9/31 (29%), Positives = 17/31 (54%) Frame = +2 Query: 257 YPIEHGIITNWDDMEKTWHHNFYNELRVAPE 349 YP G++ +W ++E N E+R+ P+ Sbjct: 244 YPRTDGLLFSWSELESMNSENMKPEIRLIPK 274 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 25.8 bits (54), Expect = 2.0 Identities = 10/36 (27%), Positives = 19/36 (52%) Frame = +2 Query: 245 PHSEYPIEHGIITNWDDMEKTWHHNFYNELRVAPEE 352 P+ PI+ G + +W+ ++ W H Y+ L P + Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPND 142 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 4.7 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +3 Query: 75 LVVDNGSGMCKAGFAG 122 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 24.2 bits (50), Expect = 6.2 Identities = 12/38 (31%), Positives = 16/38 (42%) Frame = -2 Query: 283 GDDTVLDGVLRVRMPVLLWAS*PT*ESVWPIPTITPWW 170 GDD L G R +LW P + P+P + W Sbjct: 94 GDDATLSGETIKRAAAILWHKIPEYQD-QPVPNFSNGW 130 >SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 682 Score = 23.8 bits (49), Expect = 8.2 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = -1 Query: 134 GRVVAGETGLAHAGAIVDYKRSNIVVA 54 G+V+ G++ A A DYK+ ++A Sbjct: 4 GKVIEGQSVAEWASAYFDYKKGKKIIA 30 >SPAC637.12c |mst1||histone acetyltransferase Mst1|Schizosaccharomyces pombe|chr 1|||Manual Length = 463 Score = 23.8 bits (49), Expect = 8.2 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +1 Query: 82 STMAPACARPVSPATTRPAPSS 147 S AP+ P +P+T +P PS+ Sbjct: 107 SITAPSKTEPSTPSTEKPEPST 128 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,482,031 Number of Sequences: 5004 Number of extensions: 27936 Number of successful extensions: 102 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 94 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 102 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 110009772 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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