BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0721.Seq (783 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 44 2e-05 SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual 29 0.57 SPBC28E12.02 ||SPBC9B6.13|RNA-binding protein|Schizosaccharomyce... 29 0.75 SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur... 29 0.75 SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|... 29 0.75 SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 29 0.99 SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa... 28 1.3 SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schi... 28 1.7 SPAC3A11.10c |||dipeptidyl aminopeptidase |Schizosaccharomyces p... 27 3.0 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 26 5.3 SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces po... 26 5.3 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 26 7.0 SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 26 7.0 SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 25 9.3 >SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual Length = 1279 Score = 44.0 bits (99), Expect = 2e-05 Identities = 25/82 (30%), Positives = 49/82 (59%), Gaps = 5/82 (6%) Frame = +2 Query: 269 IMAIVHQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGD---DSIVV 439 ++A+V+Q +V IDP + R +IG +GKN++++ +E++V++ ++G+ D IV Sbjct: 553 LLALVNQFPESKFYKVTIDPLLQRYVIGSKGKNLQKLRNEHQVELLVGEYGEEDPDVIVC 612 Query: 440 ITGDEDNVL--NAKEHLLNLAE 499 G +D ++ L +LAE Sbjct: 613 YIGADDGKSPDQIQKELADLAE 634 Score = 43.2 bits (97), Expect = 4e-05 Identities = 24/78 (30%), Positives = 44/78 (56%) Frame = +3 Query: 3 SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQ 182 +V K+ + + IA +EK + +++ + + H +IG GGS+I KIR V+ Sbjct: 1182 AVRGHKENVEKAIASLEKSIK-QVMENCIAYLGIPTNLHRRIIGSGGSIINKIRKIAQVK 1240 Query: 183 INLPKRGEPDDDIITIQG 236 I++P+ P D+I+ +QG Sbjct: 1241 IDVPR--TPGDEIVVVQG 1256 Score = 42.3 bits (95), Expect = 8e-05 Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 14/76 (18%) Frame = +2 Query: 314 VDIDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGDDS--------------IVVITGD 451 V+I HR+LIG GK +RR+ +++ V +RFP+ D S VVI G Sbjct: 801 VNIPNDFHRQLIGSNGKYVRRLEEKFSVRVRFPREDDSSNSTGNELMKPTSPDEVVIRGG 860 Query: 452 EDNVLNAKEHLLNLAE 499 + +V AK+ LL L E Sbjct: 861 KKSVAAAKQELLELYE 876 Score = 39.1 bits (87), Expect = 7e-04 Identities = 21/61 (34%), Positives = 31/61 (50%) Frame = +2 Query: 320 IDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGDDSIVVITGDEDNVLNAKEHLLNLAE 499 I +HRR+IG G I +I +V I P+ D IVV+ G V+ AK+ + + Sbjct: 1214 IPTNLHRRIIGSGGSIINKIRKIAQVKIDVPRTPGDEIVVVQGSRAGVVKAKDLIFERLQ 1273 Query: 500 E 502 E Sbjct: 1274 E 1274 Score = 37.9 bits (84), Expect = 0.002 Identities = 19/61 (31%), Positives = 33/61 (54%) Frame = +3 Query: 9 ENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQIN 188 ++ ++ ++E + K +L S +F +Y VIGK GS ++ +R D GVQIN Sbjct: 705 KDVERVVSEIKQVVRDAKNHEILHSHVEEFDFPAQYSKNVIGKNGSNVSSLREDLGVQIN 764 Query: 189 L 191 + Sbjct: 765 V 765 Score = 33.5 bits (73), Expect = 0.035 Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 2/78 (2%) Frame = +2 Query: 302 YREEVDIDPRVHRRLIGLRGKNIRRIMDEY--KVDIRFPKHGDDSIVVITGDEDNVLNAK 475 Y +DI + R++G G + I ++ K+DI + + V + G + +V NA Sbjct: 883 YTSTIDIPSKAVSRVVGRNGSTVENIRTQFDVKIDIGDVSTEETTPVSVRGAKADVENAI 942 Query: 476 EHLLNLAEEYLKMSQTVI 529 + + +AEE + + VI Sbjct: 943 KEISAIAEEVKNLVEKVI 960 Score = 32.3 bits (70), Expect = 0.081 Identities = 15/44 (34%), Positives = 24/44 (54%) Frame = +3 Query: 93 KFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKRGEPDDDII 224 K +DP VIG G + K+R + V++ + + GE D D+I Sbjct: 567 KVTIDPLLQRYVIGSKGKNLQKLRNEHQVELLVGEYGEEDPDVI 610 Score = 28.7 bits (61), Expect = 0.99 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = +3 Query: 126 VIGKGGSVITKIRTDFGVQINLPKRGEPDDDIITIQG 236 V+G+ GS + IRT F V+I++ + ++++G Sbjct: 897 VVGRNGSTVENIRTQFDVKIDIGDVSTEETTPVSVRG 933 Score = 28.7 bits (61), Expect = 0.99 Identities = 20/82 (24%), Positives = 40/82 (48%), Gaps = 1/82 (1%) Frame = +3 Query: 21 QALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKR 200 +AL ++ E+ +E ++++ E K +V +IG+ GS I +N+P Sbjct: 1022 EALETRLLEIVEELKNQV----EEKIEVPQRCISSIIGRMGSTRRDIERKTSTMLNIPNV 1077 Query: 201 GEPDDDI-ITIQGYEDKAHRLK 263 +P++ + ITI G + + K Sbjct: 1078 LDPEETVTITIVGSPENCEKAK 1099 Score = 27.9 bits (59), Expect = 1.7 Identities = 17/48 (35%), Positives = 23/48 (47%) Frame = +3 Query: 3 SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGS 146 SV AK + I E+ E+ + E K+D EYH +IG GS Sbjct: 930 SVRGAKADVENAIKEISAIAEE-VKNLVEKVIKIDREYHRYLIGPNGS 976 Score = 27.9 bits (59), Expect = 1.7 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = +2 Query: 269 IMAIVHQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDE 388 I AI ++ N + + ID HR LIG G ++ + E Sbjct: 945 ISAIAEEVKNLVEKVIKIDREYHRYLIGPNGSKLQNTIKE 984 Score = 26.6 bits (56), Expect = 4.0 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = +2 Query: 344 LIGLRGKNIRRIMDEYKVDIRFPKHGD 424 +IG G+N++ IMD I+ PK + Sbjct: 239 IIGTNGQNLKSIMDRTSTRIQIPKRNN 265 Score = 25.8 bits (54), Expect = 7.0 Identities = 10/40 (25%), Positives = 21/40 (52%) Frame = +2 Query: 284 HQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDEYKVDI 403 H++ + + EE D + + +IG G N+ + ++ V I Sbjct: 724 HEILHSHVEEFDFPAQYSKNVIGKNGSNVSSLREDLGVQI 763 >SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 29.5 bits (63), Expect = 0.57 Identities = 15/32 (46%), Positives = 20/32 (62%) Frame = +3 Query: 624 WTVGTTRP*HRLHSRVPDHARCSAAATLASRL 719 +T T RP RLH+ + +A SA+ LASRL Sbjct: 190 YTTETIRPHVRLHNSLSTNAALSASVVLASRL 221 >SPBC28E12.02 ||SPBC9B6.13|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 29.1 bits (62), Expect = 0.75 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +2 Query: 320 IDPRVHRRLIGLRGKNIRRIMDEYKVDIRF 409 + +H++LIG RG+ I+R+ Y I F Sbjct: 585 VPEEIHKKLIGFRGEQIQRVTKLYNSYIEF 614 >SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual Length = 398 Score = 29.1 bits (62), Expect = 0.75 Identities = 14/31 (45%), Positives = 22/31 (70%) Frame = +3 Query: 126 VIGKGGSVITKIRTDFGVQINLPKRGEPDDD 218 +IG+GGS I++IR G +I++ K EP D+ Sbjct: 335 IIGRGGSKISEIRRTSGSKISIAK--EPHDE 363 >SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|||Manual Length = 565 Score = 29.1 bits (62), Expect = 0.75 Identities = 12/37 (32%), Positives = 25/37 (67%) Frame = +3 Query: 18 KQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLV 128 KQA++E ++ ++ K+D L+ + ++ + +PEY P V Sbjct: 529 KQAVSEGMSTVDLPKDDAKLKEWIIEREWNPEYKPFV 565 >SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces pombe|chr 1|||Manual Length = 1679 Score = 28.7 bits (61), Expect = 0.99 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = -2 Query: 227 SNDIIIWLSTLG*VDLDTKVCSDFCNDRTTFSYHQRMIFWI 105 SND+I+ LS+ + D ++ SD + T Y ++ WI Sbjct: 145 SNDVILSLSSFYLIQKDQRIFSDLQFSQKTVDYRLSLLRWI 185 >SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 28.3 bits (60), Expect = 1.3 Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Frame = -1 Query: 774 KNRKIELSAVDEM--LATRSPSGWR-GLRLLSTGHGRELVSGGG 652 +NRK+ S V + + T S WR GLR ++ GH LV GG Sbjct: 63 ENRKLSPSEVGPLSIVDTSDSSWWRTGLREIARGHVAALVLAGG 106 >SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 798 Score = 27.9 bits (59), Expect = 1.7 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%) Frame = +2 Query: 323 DPRVHRRLIGLRGKNIRRIM-DEYKVDIRF 409 DP V R +I LRG NI +M ++ +DI F Sbjct: 410 DPSVCRSIIALRGYNIFGLMLKKFSIDIEF 439 >SPAC3A11.10c |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 27.1 bits (57), Expect = 3.0 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = -3 Query: 226 VMISSSGSPRLGKLIWTPKSVRIFVMTEPPFPITKG 119 +++S+ G GK IWTP+ FV+T FP+ G Sbjct: 33 LILSAVGYLGKGKWIWTPERKAHFVLTH--FPLIDG 66 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 5.3 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -3 Query: 739 DVSDAVPKRLARVAAAEHRAWSGTRE 662 +V PK+LAR A ++ AWS +E Sbjct: 499 EVEKTEPKKLARPAKIKNEAWSKQKE 524 >SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 228 Score = 26.2 bits (55), Expect = 5.3 Identities = 11/37 (29%), Positives = 24/37 (64%) Frame = +3 Query: 18 KQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLV 128 KQA++E ++ ++ K+D L+ + ++ + +PEY V Sbjct: 192 KQAISEGMSTVDLPKDDAKLKEWIIEREWNPEYRNFV 228 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.8 bits (54), Expect = 7.0 Identities = 15/60 (25%), Positives = 25/60 (41%) Frame = -3 Query: 451 ITRYHHNTVISMFRESNVNFVFVHNSPYIFTSQTNQSSVDTWVDVHFLSVLIIQLMHYCH 272 + YH+ + +E N + + SP+ F N+S+ D +LI Q CH Sbjct: 148 LRNYHYLNLDQDIQEENDSLI----SPFFFRISPNESAYDYLPPKKVSDILIEQFFFRCH 203 >SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 25.8 bits (54), Expect = 7.0 Identities = 13/39 (33%), Positives = 20/39 (51%) Frame = +3 Query: 465 LMPRNIFLIWLKNI*RCRRPLSTAGSTFFG*FRRCTGSG 581 L+P + +I ++ R L+ T FG F CTG+G Sbjct: 15 LIPTALLIIGSVSVPSTRMTLAKVEGTEFGIFGTCTGNG 53 >SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 906 Score = 25.4 bits (53), Expect = 9.3 Identities = 13/47 (27%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Frame = +3 Query: 6 VENAKQALTEKIAEMEK--EKEDRLLRSFELKFKVDPEYHPLVIGKG 140 +E+ + + K +E+ +++DR L+ E K+ P Y L + KG Sbjct: 640 IESGTERIWTKSISLERILDEKDRALQLLENALKIYPHYDKLYMMKG 686 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,265,545 Number of Sequences: 5004 Number of extensions: 72944 Number of successful extensions: 245 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 217 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 244 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 379359666 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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