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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0721.Seq
         (783 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual        44   2e-05
SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual      29   0.57 
SPBC28E12.02 ||SPBC9B6.13|RNA-binding protein|Schizosaccharomyce...    29   0.75 
SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur...    29   0.75 
SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|...    29   0.75 
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc...    29   0.99 
SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa...    28   1.3  
SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schi...    28   1.7  
SPAC3A11.10c |||dipeptidyl aminopeptidase |Schizosaccharomyces p...    27   3.0  
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy...    26   5.3  
SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces po...    26   5.3  
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ...    26   7.0  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    26   7.0  
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|...    25   9.3  

>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1279

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 25/82 (30%), Positives = 49/82 (59%), Gaps = 5/82 (6%)
 Frame = +2

Query: 269 IMAIVHQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGD---DSIVV 439
           ++A+V+Q       +V IDP + R +IG +GKN++++ +E++V++   ++G+   D IV 
Sbjct: 553 LLALVNQFPESKFYKVTIDPLLQRYVIGSKGKNLQKLRNEHQVELLVGEYGEEDPDVIVC 612

Query: 440 ITGDEDNVL--NAKEHLLNLAE 499
             G +D       ++ L +LAE
Sbjct: 613 YIGADDGKSPDQIQKELADLAE 634



 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 24/78 (30%), Positives = 44/78 (56%)
 Frame = +3

Query: 3    SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQ 182
            +V   K+ + + IA +EK  + +++ +      +    H  +IG GGS+I KIR    V+
Sbjct: 1182 AVRGHKENVEKAIASLEKSIK-QVMENCIAYLGIPTNLHRRIIGSGGSIINKIRKIAQVK 1240

Query: 183  INLPKRGEPDDDIITIQG 236
            I++P+   P D+I+ +QG
Sbjct: 1241 IDVPR--TPGDEIVVVQG 1256



 Score = 42.3 bits (95), Expect = 8e-05
 Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 14/76 (18%)
 Frame = +2

Query: 314  VDIDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGDDS--------------IVVITGD 451
            V+I    HR+LIG  GK +RR+ +++ V +RFP+  D S               VVI G 
Sbjct: 801  VNIPNDFHRQLIGSNGKYVRRLEEKFSVRVRFPREDDSSNSTGNELMKPTSPDEVVIRGG 860

Query: 452  EDNVLNAKEHLLNLAE 499
            + +V  AK+ LL L E
Sbjct: 861  KKSVAAAKQELLELYE 876



 Score = 39.1 bits (87), Expect = 7e-04
 Identities = 21/61 (34%), Positives = 31/61 (50%)
 Frame = +2

Query: 320  IDPRVHRRLIGLRGKNIRRIMDEYKVDIRFPKHGDDSIVVITGDEDNVLNAKEHLLNLAE 499
            I   +HRR+IG  G  I +I    +V I  P+   D IVV+ G    V+ AK+ +    +
Sbjct: 1214 IPTNLHRRIIGSGGSIINKIRKIAQVKIDVPRTPGDEIVVVQGSRAGVVKAKDLIFERLQ 1273

Query: 500  E 502
            E
Sbjct: 1274 E 1274



 Score = 37.9 bits (84), Expect = 0.002
 Identities = 19/61 (31%), Positives = 33/61 (54%)
 Frame = +3

Query: 9   ENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQIN 188
           ++ ++ ++E    +   K   +L S   +F    +Y   VIGK GS ++ +R D GVQIN
Sbjct: 705 KDVERVVSEIKQVVRDAKNHEILHSHVEEFDFPAQYSKNVIGKNGSNVSSLREDLGVQIN 764

Query: 189 L 191
           +
Sbjct: 765 V 765



 Score = 33.5 bits (73), Expect = 0.035
 Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 2/78 (2%)
 Frame = +2

Query: 302  YREEVDIDPRVHRRLIGLRGKNIRRIMDEY--KVDIRFPKHGDDSIVVITGDEDNVLNAK 475
            Y   +DI  +   R++G  G  +  I  ++  K+DI      + + V + G + +V NA 
Sbjct: 883  YTSTIDIPSKAVSRVVGRNGSTVENIRTQFDVKIDIGDVSTEETTPVSVRGAKADVENAI 942

Query: 476  EHLLNLAEEYLKMSQTVI 529
            + +  +AEE   + + VI
Sbjct: 943  KEISAIAEEVKNLVEKVI 960



 Score = 32.3 bits (70), Expect = 0.081
 Identities = 15/44 (34%), Positives = 24/44 (54%)
 Frame = +3

Query: 93  KFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKRGEPDDDII 224
           K  +DP     VIG  G  + K+R +  V++ + + GE D D+I
Sbjct: 567 KVTIDPLLQRYVIGSKGKNLQKLRNEHQVELLVGEYGEEDPDVI 610



 Score = 28.7 bits (61), Expect = 0.99
 Identities = 11/37 (29%), Positives = 22/37 (59%)
 Frame = +3

Query: 126  VIGKGGSVITKIRTDFGVQINLPKRGEPDDDIITIQG 236
            V+G+ GS +  IRT F V+I++      +   ++++G
Sbjct: 897  VVGRNGSTVENIRTQFDVKIDIGDVSTEETTPVSVRG 933



 Score = 28.7 bits (61), Expect = 0.99
 Identities = 20/82 (24%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
 Frame = +3

Query: 21   QALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKR 200
            +AL  ++ E+ +E ++++    E K +V       +IG+ GS    I       +N+P  
Sbjct: 1022 EALETRLLEIVEELKNQV----EEKIEVPQRCISSIIGRMGSTRRDIERKTSTMLNIPNV 1077

Query: 201  GEPDDDI-ITIQGYEDKAHRLK 263
             +P++ + ITI G  +   + K
Sbjct: 1078 LDPEETVTITIVGSPENCEKAK 1099



 Score = 27.9 bits (59), Expect = 1.7
 Identities = 17/48 (35%), Positives = 23/48 (47%)
 Frame = +3

Query: 3    SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGS 146
            SV  AK  +   I E+    E+ +    E   K+D EYH  +IG  GS
Sbjct: 930  SVRGAKADVENAIKEISAIAEE-VKNLVEKVIKIDREYHRYLIGPNGS 976



 Score = 27.9 bits (59), Expect = 1.7
 Identities = 13/40 (32%), Positives = 20/40 (50%)
 Frame = +2

Query: 269  IMAIVHQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDE 388
            I AI  ++ N   + + ID   HR LIG  G  ++  + E
Sbjct: 945  ISAIAEEVKNLVEKVIKIDREYHRYLIGPNGSKLQNTIKE 984



 Score = 26.6 bits (56), Expect = 4.0
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = +2

Query: 344 LIGLRGKNIRRIMDEYKVDIRFPKHGD 424
           +IG  G+N++ IMD     I+ PK  +
Sbjct: 239 IIGTNGQNLKSIMDRTSTRIQIPKRNN 265



 Score = 25.8 bits (54), Expect = 7.0
 Identities = 10/40 (25%), Positives = 21/40 (52%)
 Frame = +2

Query: 284 HQLDNQYREEVDIDPRVHRRLIGLRGKNIRRIMDEYKVDI 403
           H++ + + EE D   +  + +IG  G N+  + ++  V I
Sbjct: 724 HEILHSHVEEFDFPAQYSKNVIGKNGSNVSSLREDLGVQI 763


>SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 324

 Score = 29.5 bits (63), Expect = 0.57
 Identities = 15/32 (46%), Positives = 20/32 (62%)
 Frame = +3

Query: 624 WTVGTTRP*HRLHSRVPDHARCSAAATLASRL 719
           +T  T RP  RLH+ +  +A  SA+  LASRL
Sbjct: 190 YTTETIRPHVRLHNSLSTNAALSASVVLASRL 221


>SPBC28E12.02 ||SPBC9B6.13|RNA-binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 663

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +2

Query: 320 IDPRVHRRLIGLRGKNIRRIMDEYKVDIRF 409
           +   +H++LIG RG+ I+R+   Y   I F
Sbjct: 585 VPEEIHKKLIGFRGEQIQRVTKLYNSYIEF 614


>SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin
           deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 398

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 14/31 (45%), Positives = 22/31 (70%)
 Frame = +3

Query: 126 VIGKGGSVITKIRTDFGVQINLPKRGEPDDD 218
           +IG+GGS I++IR   G +I++ K  EP D+
Sbjct: 335 IIGRGGSKISEIRRTSGSKISIAK--EPHDE 363


>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 565

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 12/37 (32%), Positives = 25/37 (67%)
 Frame = +3

Query: 18  KQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLV 128
           KQA++E ++ ++  K+D  L+ + ++ + +PEY P V
Sbjct: 529 KQAVSEGMSTVDLPKDDAKLKEWIIEREWNPEYKPFV 565


>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1679

 Score = 28.7 bits (61), Expect = 0.99
 Identities = 13/41 (31%), Positives = 22/41 (53%)
 Frame = -2

Query: 227 SNDIIIWLSTLG*VDLDTKVCSDFCNDRTTFSYHQRMIFWI 105
           SND+I+ LS+   +  D ++ SD    + T  Y   ++ WI
Sbjct: 145 SNDVILSLSSFYLIQKDQRIFSDLQFSQKTVDYRLSLLRWI 185


>SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
 Frame = -1

Query: 774 KNRKIELSAVDEM--LATRSPSGWR-GLRLLSTGHGRELVSGGG 652
           +NRK+  S V  +  + T   S WR GLR ++ GH   LV  GG
Sbjct: 63  ENRKLSPSEVGPLSIVDTSDSSWWRTGLREIARGHVAALVLAGG 106


>SPAC29B12.02c |set2||histone lysine methyltransferase Set2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 798

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
 Frame = +2

Query: 323 DPRVHRRLIGLRGKNIRRIM-DEYKVDIRF 409
           DP V R +I LRG NI  +M  ++ +DI F
Sbjct: 410 DPSVCRSIIALRGYNIFGLMLKKFSIDIEF 439


>SPAC3A11.10c |||dipeptidyl aminopeptidase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 409

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 14/36 (38%), Positives = 21/36 (58%)
 Frame = -3

Query: 226 VMISSSGSPRLGKLIWTPKSVRIFVMTEPPFPITKG 119
           +++S+ G    GK IWTP+    FV+T   FP+  G
Sbjct: 33  LILSAVGYLGKGKWIWTPERKAHFVLTH--FPLIDG 66


>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -3

Query: 739 DVSDAVPKRLARVAAAEHRAWSGTRE 662
           +V    PK+LAR A  ++ AWS  +E
Sbjct: 499 EVEKTEPKKLARPAKIKNEAWSKQKE 524


>SPAC750.08c |||NAD-dependent malic enzyme|Schizosaccharomyces
           pombe|chr 1||Partial|Manual
          Length = 228

 Score = 26.2 bits (55), Expect = 5.3
 Identities = 11/37 (29%), Positives = 24/37 (64%)
 Frame = +3

Query: 18  KQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLV 128
           KQA++E ++ ++  K+D  L+ + ++ + +PEY   V
Sbjct: 192 KQAISEGMSTVDLPKDDAKLKEWIIEREWNPEYRNFV 228


>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 15/60 (25%), Positives = 25/60 (41%)
 Frame = -3

Query: 451 ITRYHHNTVISMFRESNVNFVFVHNSPYIFTSQTNQSSVDTWVDVHFLSVLIIQLMHYCH 272
           +  YH+  +    +E N + +    SP+ F    N+S+ D         +LI Q    CH
Sbjct: 148 LRNYHYLNLDQDIQEENDSLI----SPFFFRISPNESAYDYLPPKKVSDILIEQFFFRCH 203


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = +3

Query: 465 LMPRNIFLIWLKNI*RCRRPLSTAGSTFFG*FRRCTGSG 581
           L+P  + +I   ++   R  L+    T FG F  CTG+G
Sbjct: 15  LIPTALLIIGSVSVPSTRMTLAKVEGTEFGIFGTCTGNG 53


>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
           Prp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 906

 Score = 25.4 bits (53), Expect = 9.3
 Identities = 13/47 (27%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
 Frame = +3

Query: 6   VENAKQALTEKIAEMEK--EKEDRLLRSFELKFKVDPEYHPLVIGKG 140
           +E+  + +  K   +E+  +++DR L+  E   K+ P Y  L + KG
Sbjct: 640 IESGTERIWTKSISLERILDEKDRALQLLENALKIYPHYDKLYMMKG 686


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,265,545
Number of Sequences: 5004
Number of extensions: 72944
Number of successful extensions: 245
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 244
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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