Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0721.Seq
(783 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15270.2 68418.m01789 KH domain-containing protein various pr... 39 0.004
At5g15270.1 68418.m01788 KH domain-containing protein various pr... 39 0.004
At5g46190.1 68418.m05681 KH domain-containing protein strong sim... 36 0.040
At5g64390.2 68418.m08088 KH domain-containing RNA-binding protei... 33 0.21
At5g64390.1 68418.m08089 KH domain-containing RNA-binding protei... 33 0.21
At4g18375.2 68417.m02727 KH domain-containing protein contains s... 31 0.65
At4g18375.1 68417.m02726 KH domain-containing protein contains s... 31 0.65
At5g04430.2 68418.m00438 KH domain-containing protein NOVA, puta... 31 0.86
At5g04430.1 68418.m00437 KH domain-containing protein NOVA, puta... 31 0.86
At1g14170.1 68414.m01675 KH domain-containing protein location o... 31 0.86
At2g21510.1 68415.m02560 DNAJ heat shock N-terminal domain-conta... 29 3.5
At1g75640.1 68414.m08788 leucine-rich repeat family protein / pr... 29 4.6
At1g68790.1 68414.m07863 expressed protein 29 4.6
At5g52870.1 68418.m06562 expressed protein similar to unknown pr... 28 6.1
At5g42390.1 68418.m05161 metalloendopeptidase identical to chlor... 28 6.1
At1g12160.1 68414.m01408 flavin-containing monooxygenase family ... 28 6.1
At5g03830.1 68418.m00352 expressed protein hypothetical protein ... 28 8.0
At4g39150.1 68417.m05545 DNAJ heat shock N-terminal domain-conta... 28 8.0
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 28 8.0
>At5g15270.2 68418.m01789 KH domain-containing protein various
predicted proteins, Arabidopsis thaliana and Oryza
sativa
Length = 548
Score = 38.7 bits (86), Expect = 0.004
Identities = 25/73 (34%), Positives = 39/73 (53%), Gaps = 6/73 (8%)
Frame = +3
Query: 51 EKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIR--TDFGVQI----NLPKRGEPD 212
+K + D L SF + V ++GKGG++IT++R T ++I NLPK D
Sbjct: 359 DKVERDSGLVSFTTRLLVPSSRIGCILGKGGAIITEMRRMTKANIRILGKENLPKVASDD 418
Query: 213 DDIITIQGYEDKA 251
D+++ I G D A
Sbjct: 419 DEMVQISGELDVA 431
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQINL-PKRGEPDDDIITIQGYE 242
VIGKGG++I ++R + I + R E +D +ITI E
Sbjct: 298 VIGKGGALINQLRQETRATIKVDSSRTEGNDCLITISARE 337
>At5g15270.1 68418.m01788 KH domain-containing protein various
predicted proteins, Arabidopsis thaliana and Oryza
sativa
Length = 548
Score = 38.7 bits (86), Expect = 0.004
Identities = 25/73 (34%), Positives = 39/73 (53%), Gaps = 6/73 (8%)
Frame = +3
Query: 51 EKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIR--TDFGVQI----NLPKRGEPD 212
+K + D L SF + V ++GKGG++IT++R T ++I NLPK D
Sbjct: 359 DKVERDSGLVSFTTRLLVPSSRIGCILGKGGAIITEMRRMTKANIRILGKENLPKVASDD 418
Query: 213 DDIITIQGYEDKA 251
D+++ I G D A
Sbjct: 419 DEMVQISGELDVA 431
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQINL-PKRGEPDDDIITIQGYE 242
VIGKGG++I ++R + I + R E +D +ITI E
Sbjct: 298 VIGKGGALINQLRQETRATIKVDSSRTEGNDCLITISARE 337
>At5g46190.1 68418.m05681 KH domain-containing protein strong
similarity to unknown protein (pir||T04533)
Length = 644
Score = 35.5 bits (78), Expect = 0.040
Identities = 22/84 (26%), Positives = 43/84 (51%), Gaps = 6/84 (7%)
Frame = +3
Query: 3 SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKI--RTDFG 176
S ++ K E + ++++ D +++ V + +IGK GS+I++I RT
Sbjct: 372 SPDDLKSMAVEAVLLLQEKINDEDEDKVKMQLLVSSKVIGCIIGKSGSIISEIRKRTKAD 431
Query: 177 VQI----NLPKRGEPDDDIITIQG 236
+ I N PK +P+D+++ I G
Sbjct: 432 IHISKGNNTPKCADPNDELVEISG 455
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFG--VQINLPKRGEPDDDIITIQGYE 242
VIGKGG I IR G +++N + DD +IT+ E
Sbjct: 331 VIGKGGLTIKGIRQASGSHIEVNDSRTNHDDDCVITVTATE 371
>At5g64390.2 68418.m08088 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 824
Score = 33.1 bits (72), Expect = 0.21
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQI-----NLPKRGEPDDDIITIQG 236
VIGKGG ++ IR + G +I NLP + DD+++ ++G
Sbjct: 164 VIGKGGQMVGSIRKETGCKISIRIENLPICADTDDEMVEVEG 205
>At5g64390.1 68418.m08089 KH domain-containing RNA-binding protein
(HEN4) contains similarity to RNA-binding protein;
identical to cDNA HEN4 isoform 2 (HEN4) GI:28261404;
contains Pfam domain PF00013: KH domain; identical to
cDNA HEN4 (HEN4) GI:28261402
Length = 857
Score = 33.1 bits (72), Expect = 0.21
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 5/42 (11%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQI-----NLPKRGEPDDDIITIQG 236
VIGKGG ++ IR + G +I NLP + DD+++ ++G
Sbjct: 164 VIGKGGQMVGSIRKETGCKISIRIENLPICADTDDEMVEVEG 205
>At4g18375.2 68417.m02727 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 606
Score = 31.5 bits (68), Expect = 0.65
Identities = 21/78 (26%), Positives = 38/78 (48%)
Frame = +3
Query: 3 SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQ 182
S ++ K E + +++ D + +++ V + VIGK GSVI +IR
Sbjct: 368 SPDDMKSMAVEAVLLLQEYINDEDAENVKMQLLVSSKVIGCVIGKSGSVINEIRKRTNAN 427
Query: 183 INLPKRGEPDDDIITIQG 236
I + K G+ DD++ + G
Sbjct: 428 ICISK-GK-KDDLVEVSG 443
Score = 29.5 bits (63), Expect = 2.6
Identities = 23/55 (41%), Positives = 32/55 (58%), Gaps = 4/55 (7%)
Frame = +3
Query: 78 RSFELKFKV-DPEYHPL-VIGKGGSVITKIRTDFG--VQINLPKRGEPDDDIITI 230
RS EL FKV P + + VIGKGGS I +IR G +++N + DD+ + I
Sbjct: 308 RSEELVFKVLCPLCNIMRVIGKGGSTIKRIREASGSCIEVNDSRTKCGDDECVII 362
>At4g18375.1 68417.m02726 KH domain-containing protein contains
similarity to RNA-binding KH-domains PF:00013
Length = 532
Score = 31.5 bits (68), Expect = 0.65
Identities = 21/78 (26%), Positives = 38/78 (48%)
Frame = +3
Query: 3 SVENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQ 182
S ++ K E + +++ D + +++ V + VIGK GSVI +IR
Sbjct: 368 SPDDMKSMAVEAVLLLQEYINDEDAENVKMQLLVSSKVIGCVIGKSGSVINEIRKRTNAN 427
Query: 183 INLPKRGEPDDDIITIQG 236
I + K G+ DD++ + G
Sbjct: 428 ICISK-GK-KDDLVEVSG 443
Score = 29.5 bits (63), Expect = 2.6
Identities = 23/55 (41%), Positives = 32/55 (58%), Gaps = 4/55 (7%)
Frame = +3
Query: 78 RSFELKFKV-DPEYHPL-VIGKGGSVITKIRTDFG--VQINLPKRGEPDDDIITI 230
RS EL FKV P + + VIGKGGS I +IR G +++N + DD+ + I
Sbjct: 308 RSEELVFKVLCPLCNIMRVIGKGGSTIKRIREASGSCIEVNDSRTKCGDDECVII 362
>At5g04430.2 68418.m00438 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 334
Score = 31.1 bits (67), Expect = 0.86
Identities = 21/66 (31%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Frame = +3
Query: 51 EKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKRGE----PDDD 218
+ + D + ++F V VIGKGGS IT+ + G +I L + E D
Sbjct: 24 DSSEADSAEKPTHIRFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQEFFPGTTDR 83
Query: 219 IITIQG 236
II I G
Sbjct: 84 IIMISG 89
>At5g04430.1 68418.m00437 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 313
Score = 31.1 bits (67), Expect = 0.86
Identities = 21/66 (31%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Frame = +3
Query: 51 EKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQINLPKRGE----PDDD 218
+ + D + ++F V VIGKGGS IT+ + G +I L + E D
Sbjct: 24 DSSEADSAEKPTHIRFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQEFFPGTTDR 83
Query: 219 IITIQG 236
II I G
Sbjct: 84 IIMISG 89
>At1g14170.1 68414.m01675 KH domain-containing protein location of
EST 219C14T7 , gb|N38506
Length = 454
Score = 31.1 bits (67), Expect = 0.86
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQINLPKRGEPDDDIITI----QGYEDKA 251
VIGKGG I +IR + G I + DDD I + YED++
Sbjct: 258 VIGKGGGFINQIRQETGATIRVNTSETDDDDCIIFISSKEFYEDQS 303
Score = 29.9 bits (64), Expect = 2.0
Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 6/53 (11%)
Frame = +3
Query: 126 VIGKGGSVITKIR--TDFGVQI----NLPKRGEPDDDIITIQGYEDKAHRLKT 266
+IGKGG+VI+++R T ++I ++PK D++++ I G D A + T
Sbjct: 343 LIGKGGAVISEMRSVTRANIRILQKEDVPKIAREDEEMVQITGSPDAAMKALT 395
Score = 28.7 bits (61), Expect = 4.6
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 126 VIGKGGSVITKIRTDFGVQINLPK 197
VIGKGG VI +R D QI + K
Sbjct: 135 VIGKGGQVIQNLRNDTNAQIRVIK 158
>At2g21510.1 68415.m02560 DNAJ heat shock N-terminal
domain-containing protein similar to SP|P39101 CAJ1
protein, Saccharomyces cerevisiae; contains Pfam profile
PF00226 DnaJ domain
Length = 346
Score = 29.1 bits (62), Expect = 3.5
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +3
Query: 9 ENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDP 110
E KQ L +KI ++KE+ED+L + LK K++P
Sbjct: 121 EIRKQMLQDKIKALQKEREDKLAAT--LKNKLEP 152
>At1g75640.1 68414.m08788 leucine-rich repeat family protein /
protein kinase family protein contains protein kinase
domain, Pfam:PF00069; contains leucine-rich repeats,
Pfam:PF00560
Length = 1140
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = -3
Query: 457 VFITRYHHNTVISMFRESNVNFVFVHNSPYIFTSQTNQSSVDTWVDVHFLS 305
+F +R+HH + IS ++ +F + P NQSS D H +S
Sbjct: 14 IFFSRFHHTSAISSETQALTSFKLSLHDPLGALESWNQSSPSAPCDWHGVS 64
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 28.7 bits (61), Expect = 4.6
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +3
Query: 165 TDFGVQINLPKRGEPDDDIITIQG 236
TD G+ + LP+ +PDD + + G
Sbjct: 796 TDLGITVKLPESSQPDDSLDRVSG 819
>At5g52870.1 68418.m06562 expressed protein similar to unknown
protein (gb|AAF24565.1)
Length = 326
Score = 28.3 bits (60), Expect = 6.1
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = +3
Query: 30 TEKIAEMEKEKEDRLLRSFELKFKVDPEYHPLVIGKGGSVITKIRTD 170
+EK EK+KE+ S ++F+VD E K S+ +TD
Sbjct: 118 SEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTD 164
>At5g42390.1 68418.m05161 metalloendopeptidase identical to
chloroplast processing enzyme metalloendopeptidase
[Arabidopsis thaliana] gi|2827039|gb|AAC39482
Length = 1265
Score = 28.3 bits (60), Expect = 6.1
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +1
Query: 508 KDVADRYQPPAAPSLGDFGAVLGAENPNAPAGPG 609
K+ D P++PS G FGA+ P PAG G
Sbjct: 403 KNGLDNESTPSSPSPGAFGAMANFLVPKLPAGLG 436
>At1g12160.1 68414.m01408 flavin-containing monooxygenase family
protein / FMO family protein similar to
flavin-containing monooxygenase FMO2 from Homo sapiens
[SP|Q99518]; contains Pfam profile PF00743
Flavin-binding monooxygenase-like
Length = 468
Score = 28.3 bits (60), Expect = 6.1
Identities = 17/74 (22%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
Frame = -3
Query: 541 LPAVDNGLRHL*IFFSQIKKMFLGIKYIVFITRYHHNTVISMFRESNVNFVFVHNSPYI- 365
LPA D + F+S+++ + + +Y F+T N ++ F+ + V + S Y
Sbjct: 350 LPAEDKMMEEAVAFYSKLEDLGIPKRYTHFLTDPRGNPMLGTFKPEDA--VVISQSDYFN 407
Query: 364 -FTSQTNQSSVDTW 326
Q +S++ W
Sbjct: 408 WIAKQCGCTSIERW 421
>At5g03830.1 68418.m00352 expressed protein hypothetical protein
F4I1.32, Arabidopsis thaliana, PIR:T02406
Length = 265
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +2
Query: 395 VDIRFPKHGDDSIVVITGDEDNVLNAKEHLLNLAEEYLKMSQTVINRRQHLLWVISALYW 574
VD+ + ++V + DED L A LNLA V+N Q L+V + +
Sbjct: 77 VDLILNQTALGTVVKVADDEDEALFALITALNLARYKASHLVEVLNDVQRCLFVFAIMLM 136
Query: 575 ERK 583
E K
Sbjct: 137 ENK 139
>At4g39150.1 68417.m05545 DNAJ heat shock N-terminal
domain-containing protein similar to SP|P39101 CAJ1
protein, Saccharomyces cerevisiae, PIR2:S48085; contains
Pfam profile PF00226 DnaJ domain
Length = 345
Score = 27.9 bits (59), Expect = 8.0
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +3
Query: 9 ENAKQALTEKIAEMEKEKEDRLLRSFELKFKVDP 110
E KQ L EKI ++K++ D+L+ + LK K++P
Sbjct: 121 EIRKQMLQEKIKAIQKDRVDKLVTT--LKIKLEP 152
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 27.9 bits (59), Expect = 8.0
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +3
Query: 87 ELKFKVDPEYHPLVIGKGGSVITKIRTDFGVQINL-PKRGEPD 212
+++ KV + L+IG+GG I ++T G + L P+ E D
Sbjct: 273 QIEIKVPNDKVGLIIGRGGETIKNMQTRSGARTQLIPQHAEGD 315
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,336,171
Number of Sequences: 28952
Number of extensions: 396575
Number of successful extensions: 1292
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1288
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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