BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0714.Seq (772 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 175 6e-45 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 102 5e-23 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 102 7e-23 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 97 2e-21 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 72 1e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 67 3e-12 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 61 2e-10 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 49 8e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 49 8e-07 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 34 0.026 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.32 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 29 0.74 SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 27 3.0 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.9 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 5.2 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 26 6.9 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 25 9.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 175 bits (426), Expect = 6e-45 Identities = 78/85 (91%), Positives = 82/85 (96%) Frame = +3 Query: 255 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 434 PIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN+ Sbjct: 70 PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129 Query: 435 PAMYVAIQAVLSLYASGRTTGIVLD 509 PA YVAIQAVLSLYASGRTTGIVLD Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLD 154 Score = 141 bits (341), Expect = 1e-34 Identities = 62/69 (89%), Positives = 68/69 (98%) Frame = +1 Query: 52 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 231 ++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 232 RGILTLKYP 258 RGILTLKYP Sbjct: 62 RGILTLKYP 70 Score = 133 bits (322), Expect = 2e-32 Identities = 61/66 (92%), Positives = 65/66 (98%) Frame = +2 Query: 509 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 688 SGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKE Sbjct: 155 SGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKE 214 Query: 689 KLCYVA 706 KLCYVA Sbjct: 215 KLCYVA 220 Score = 32.7 bits (71), Expect = 0.060 Identities = 15/19 (78%), Positives = 17/19 (89%) Frame = +1 Query: 706 LDFEQEMATAAASTSLEKS 762 LDFEQE+ TAA S+SLEKS Sbjct: 221 LDFEQELQTAAQSSSLEKS 239 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 102 bits (245), Expect = 5e-23 Identities = 43/85 (50%), Positives = 65/85 (76%) Frame = +3 Query: 255 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 434 P+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE + Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129 Query: 435 PAMYVAIQAVLSLYASGRTTGIVLD 509 +YVAIQAVLSLYA G ++G+V+D Sbjct: 130 GGVYVAIQAVLSLYAQGLSSGVVVD 154 Score = 87.8 bits (208), Expect = 1e-18 Identities = 38/66 (57%), Positives = 50/66 (75%) Frame = +2 Query: 509 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 688 SGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KE Sbjct: 155 SGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKE 214 Query: 689 KLCYVA 706 KLCYV+ Sbjct: 215 KLCYVS 220 Score = 64.5 bits (150), Expect = 2e-11 Identities = 33/67 (49%), Positives = 44/67 (65%), Gaps = 2/67 (2%) Frame = +1 Query: 64 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 237 A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63 Query: 238 ILTLKYP 258 +L +KYP Sbjct: 64 LLQVKYP 70 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 102 bits (244), Expect = 7e-23 Identities = 47/86 (54%), Positives = 62/86 (72%), Gaps = 1/86 (1%) Frame = +3 Query: 255 PIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN 431 PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET N Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLN 133 Query: 432 SPAMYVAIQAVLSLYASGRTTGIVLD 509 PA+ ++Q VL+LYAS RTTGIVL+ Sbjct: 134 VPALSFSLQPVLALYASARTTGIVLE 159 Score = 86.2 bits (204), Expect = 5e-18 Identities = 36/66 (54%), Positives = 53/66 (80%) Frame = +2 Query: 512 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEK 691 GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK Sbjct: 161 GDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEK 220 Query: 692 LCYVAS 709 CYVAS Sbjct: 221 CCYVAS 226 Score = 79.0 bits (186), Expect = 7e-16 Identities = 35/67 (52%), Positives = 47/67 (70%) Frame = +1 Query: 58 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 237 D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Query: 238 ILTLKYP 258 +L ++ P Sbjct: 68 LLKIQRP 74 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 97.5 bits (232), Expect = 2e-21 Identities = 42/78 (53%), Positives = 53/78 (67%) Frame = +3 Query: 249 EVPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 428 + PI HG I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+F Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESF 150 Query: 429 NSPAMYVAIQAVLSLYAS 482 N +Y+A+QAVL+L AS Sbjct: 151 NCAGLYIAVQAVLALAAS 168 Score = 55.6 bits (128), Expect = 7e-09 Identities = 30/67 (44%), Positives = 42/67 (62%), Gaps = 2/67 (2%) Frame = +2 Query: 509 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDI 682 SGDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER I Sbjct: 186 SGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----I 240 Query: 683 KEKLCYV 703 KE+ CYV Sbjct: 241 KEECCYV 247 Score = 42.7 bits (96), Expect = 6e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 70 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 219 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 71.7 bits (168), Expect = 1e-13 Identities = 32/84 (38%), Positives = 48/84 (57%) Frame = +3 Query: 258 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSP 437 I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MFE+ P Sbjct: 69 IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCP 128 Query: 438 AMYVAIQAVLSLYASGRTTGIVLD 509 A Y+A Q + +ASG+ T ++D Sbjct: 129 ATYLAKQETCAAFASGKGTACLVD 152 Score = 40.7 bits (91), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 55 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 156 D+V+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 66.9 bits (156), Expect = 3e-12 Identities = 32/87 (36%), Positives = 54/87 (62%), Gaps = 2/87 (2%) Frame = +3 Query: 255 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNS 434 P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+ Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 435 PAMYVAIQAVLSLYASGR--TTGIVLD 509 P++ I + S Y + + ++GIVL+ Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLN 171 Score = 37.1 bits (82), Expect = 0.003 Identities = 20/55 (36%), Positives = 33/55 (60%) Frame = +1 Query: 1 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 165 E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 Score = 28.3 bits (60), Expect = 1.3 Identities = 15/66 (22%), Positives = 27/66 (40%) Frame = +2 Query: 512 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEK 691 G+ SH +P+ G + R+ G + YL+K+ + SF + Sbjct: 173 GNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHD 232 Query: 692 LCYVAS 709 C+V+S Sbjct: 233 HCHVSS 238 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 60.9 bits (141), Expect = 2e-10 Identities = 25/82 (30%), Positives = 49/82 (59%) Frame = +3 Query: 264 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAM 443 +GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 444 YVAIQAVLSLYASGRTTGIVLD 509 Y+ QAV +A+ ++T +++D Sbjct: 134 YLTKQAVCVAFANSKSTALIVD 155 Score = 36.3 bits (80), Expect = 0.005 Identities = 18/57 (31%), Positives = 33/57 (57%) Frame = +1 Query: 55 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 225 +++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 48.8 bits (111), Expect = 8e-07 Identities = 23/66 (34%), Positives = 40/66 (60%) Frame = +2 Query: 509 SGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 688 SG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKE 220 Query: 689 KLCYVA 706 +CYV+ Sbjct: 221 SVCYVS 226 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 48.8 bits (111), Expect = 8e-07 Identities = 28/94 (29%), Positives = 45/94 (47%), Gaps = 4/94 (4%) Frame = +3 Query: 240 PHSEVPIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 407 P+ PI+ G + +W+ ++ W H Y+ L P + +PV L +RE T Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166 Query: 408 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLD 509 Q FE P +A + ++ LYA G G+V+D Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIGILHGLVID 200 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 33.9 bits (74), Expect = 0.026 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +1 Query: 115 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPSSTVS 273 G+ PRA F ++ P H G+++ M KD G+E S +G + + P + +S Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEIS 552 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 30.3 bits (65), Expect = 0.32 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +3 Query: 315 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 458 +Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 29.1 bits (62), Expect = 0.74 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -1 Query: 772 ESSWTSRGRWRRQRWPSPARSRGDIAELLLDV 677 ++S ++R + RR +W R RGD E+LL V Sbjct: 438 DTSQSTRKKSRRNKWHFGVRCRGDAPEILLAV 469 >SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 1405 Score = 27.1 bits (57), Expect = 3.0 Identities = 17/42 (40%), Positives = 22/42 (52%), Gaps = 8/42 (19%) Frame = -1 Query: 766 SWTSRGRWRRQRWP-SPARSRGDIAE-------LLLDVTNDF 665 +W+S GR RR WP S RS E L+ D++NDF Sbjct: 644 NWSSIGRLRRSEWPRSFNRSSQQFVEKQIIAGRLMCDLSNDF 685 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.9 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +3 Query: 540 STKVTLCPTPSSVWTW 587 STK CP PS W W Sbjct: 52 STKTATCPDPSWEWAW 67 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 5.2 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 357 LLTEAPLNPKANREKMTQIMFETFN 431 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 25.8 bits (54), Expect = 6.9 Identities = 14/47 (29%), Positives = 29/47 (61%), Gaps = 3/47 (6%) Frame = +3 Query: 252 VPIEHGIITNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAPLNP 383 VP + +I + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 265 VPQGNFVIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.4 bits (53), Expect = 9.1 Identities = 9/36 (25%), Positives = 21/36 (58%) Frame = +2 Query: 539 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 646 + EG +P++ ++++ G D+T MK+L + + Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,023,681 Number of Sequences: 5004 Number of extensions: 63432 Number of successful extensions: 256 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 222 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 251 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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