SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= pg--0712.Seq
         (571 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g38150.1 68418.m04598 expressed protein                             31   0.72 
At4g32030.1 68417.m04560 expressed protein                             31   0.72 
At1g67890.1 68414.m07752 protein kinase family protein contains ...    30   0.95 
At1g25540.1 68414.m03171 phytochrome and flowering time regulato...    29   2.2  
At5g16730.1 68418.m01959 expressed protein weak similarity to mi...    29   2.9  
At4g39610.1 68417.m05599 expressed protein contains Pfam profile...    28   3.8  
At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing ...    28   5.0  
At3g50240.1 68416.m05494 kinesin motor protein-related KINESIN-L...    27   6.7  
At3g10730.1 68416.m01292 sad1/unc-84-like 2 family protein conta...    27   6.7  
At3g05270.1 68416.m00575 expressed protein similar to endosome-a...    27   6.7  
At3g01810.1 68416.m00123 expressed protein                             27   6.7  
At3g22790.1 68416.m02873 kinase interacting family protein simil...    27   8.8  
At2g45290.1 68415.m05637 transketolase, putative strong similari...    27   8.8  
At2g07020.1 68415.m00803 protein kinase family protein contains ...    27   8.8  

>At5g38150.1 68418.m04598 expressed protein
          Length = 574

 Score = 30.7 bits (66), Expect = 0.72
 Identities = 18/67 (26%), Positives = 35/67 (52%), Gaps = 2/67 (2%)
 Frame = +1

Query: 19  KMKSLVERIRLENTTLKKSLDIERSRVRALKARYDSALRNLKTEHR--KREEFLEKQLRT 192
           K++ L  +I +E + L+  + I   R+ +L   +  +L  +K      K+EEFL K+ +T
Sbjct: 313 KIEKLNSKILIEKSKLEV-VSIAEERISSLAENFVGSLEKIKKSRNAAKKEEFLFKEEKT 371

Query: 193 IARPHKQ 213
           + +   Q
Sbjct: 372 VTKAETQ 378


>At4g32030.1 68417.m04560 expressed protein
          Length = 253

 Score = 30.7 bits (66), Expect = 0.72
 Identities = 19/50 (38%), Positives = 29/50 (58%)
 Frame = +1

Query: 13  LTKMKSLVERIRLENTTLKKSLDIERSRVRALKARYDSALRNLKTEHRKR 162
           L K KS  E    EN  LK+ LD+E+  + +L+A +D   +NL+ +  KR
Sbjct: 143 LKKRKSSFELKNEENLKLKERLDLEK-EIASLRATFDE--QNLRNQKLKR 189


>At1g67890.1 68414.m07752 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 765

 Score = 30.3 bits (65), Expect = 0.95
 Identities = 16/38 (42%), Positives = 22/38 (57%)
 Frame = +3

Query: 429 KLLEEIKSKERTIALLKREAAKSTAQQTIRRREWSSEP 542
           KLL++I+  E +   LKRE +K      I+RR  SS P
Sbjct: 10  KLLKKIRELEESQEDLKREMSKLKVSAEIKRRSHSSSP 47


>At1g25540.1 68414.m03171 phytochrome and flowering time regulatory
           protein (PFT1) PMID: 12815435
          Length = 836

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 12/42 (28%), Positives = 25/42 (59%)
 Frame = +3

Query: 15  HQNEEPRRKDKAGEHDLEEVARHRAEQSQST*SPL*QRSQES 140
           HQ ++ +++ +  +H L ++  H  +Q Q+  SPL Q  Q++
Sbjct: 740 HQQQQQQQQQQQQQHQLTQLQHHHQQQQQA--SPLNQMQQQT 779


>At5g16730.1 68418.m01959 expressed protein weak similarity to
           microtubule binding protein D-CLIP-190 [Drosophila
           melanogaster] GI:2773363, SMC2-like condensin
           [Arabidopsis thaliana] GI:14279543
          Length = 853

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 21/67 (31%), Positives = 37/67 (55%), Gaps = 1/67 (1%)
 Frame = +1

Query: 1   DESLLTKMKSLVERIRLE-NTTLKKSLDIERSRVRALKARYDSALRNLKTEHRKREEFLE 177
           +E  + K+KS +E ++ E N  LKK  D   SRV+ L       L +L++  ++ EE  +
Sbjct: 406 NEKEVEKLKSELETVKEEKNRALKKEQD-ATSRVQRLSEEKSKLLSDLESS-KEEEEKSK 463

Query: 178 KQLRTIA 198
           K + ++A
Sbjct: 464 KAMESLA 470


>At4g39610.1 68417.m05599 expressed protein contains Pfam profile
           PF04759: Protein of unknown function, DUF617
          Length = 264

 Score = 28.3 bits (60), Expect = 3.8
 Identities = 17/58 (29%), Positives = 30/58 (51%)
 Frame = +1

Query: 22  MKSLVERIRLENTTLKKSLDIERSRVRALKARYDSALRNLKTEHRKREEFLEKQLRTI 195
           + SLV  + ++ TTL+K L     R+ AL+        N  ++  K+ + LE+ L T+
Sbjct: 120 LPSLVVELAMQTTTLQKELSTGMVRI-ALETEKQPRADNNNSKTEKKTDILEEPLWTM 176


>At3g23900.1 68416.m03003 RNA recognition motif (RRM)-containing
            protein 
          Length = 987

 Score = 27.9 bits (59), Expect = 5.0
 Identities = 31/113 (27%), Positives = 49/113 (43%), Gaps = 7/113 (6%)
 Frame = +1

Query: 67   KKSLDIER---SRVRALKARYDSALRNLKTEHRKREEFLEKQL----RTIARPHKQTDDA 225
            K  LD +R   SR R  +++     R+   E R R++  +++     R+ +   KQ  D 
Sbjct: 775  KNKLDEDRNTGSRRRRSRSKSVEGKRSYNKETRSRDKKSKRRSGRRSRSPSSEGKQGRDI 834

Query: 226  QSACGRLTDVKD*QQRFSL*KQLIRVFKKNLRSHNRRSAHARLSSGPRRRDTR 384
            +S+ G   + K   +R S  +    + KKN     R   H RL S    RD R
Sbjct: 835  RSSPGYSDEKKSRHKRHSRSRS---IEKKNSSRDKRSKRHERLRSSSPGRDKR 884



 Score = 27.5 bits (58), Expect = 6.7
 Identities = 38/163 (23%), Positives = 69/163 (42%), Gaps = 8/163 (4%)
 Frame = +1

Query: 1    DESLLTKMKSLVERIRLENTTLKKSLDIERSRVRALKARYDSALRNLKTEHRKREEFLEK 180
            ++S   K KS  E ++      +     +RS+ R      D A    K +HR+R     +
Sbjct: 663  EDSADIKDKSRDEELKHHKKRSRSRSREDRSKTRDTSRNSDEA----KQKHRQRSR--SR 716

Query: 181  QLRTIARPHKQTDDAQ----SACGRLTDVKD*QQRFSL*KQLIRVFKKNLRSHNRRSAHA 348
             L      H+  D AQ    ++       K   + + + ++  R   ++L + NR S   
Sbjct: 717  SLENDNGSHENVDVAQDNDLNSRHSKRRSKSLDEDYDMKERRGRSRSRSLETKNRSSRKN 776

Query: 349  RL----SSGPRRRDTRPWSKPHEGMHVRNAINCWRKSNRRSGQ 465
            +L    ++G RRR +R  S   +  + +   +  +KS RRSG+
Sbjct: 777  KLDEDRNTGSRRRRSRSKSVEGKRSYNKETRSRDKKSKRRSGR 819


>At3g50240.1 68416.m05494 kinesin motor protein-related KINESIN-LIKE
           PROTEIN KIF4, Homo sapiens, EMBL:AF179308
          Length = 1051

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 15/28 (53%), Positives = 19/28 (67%)
 Frame = +3

Query: 255 KRLTTEVQSLKAAYKGLQEKLKVAQSAE 338
           KRL TE+Q +KA    LQ+K+K  Q AE
Sbjct: 622 KRLKTEIQCIKAQKVQLQQKMK--QEAE 647


>At3g10730.1 68416.m01292 sad1/unc-84-like 2 family protein contains
           1 transmembrane domain; similar to Sad1 unc-84 domain
           protein 2 (GI:6538749) [Homo sapiens]; similar to
           Sad1/unc-84-like protein 2 (Fragment)
           (Swiss-Prot:Q9UH99) [Homo sapiens]
          Length = 455

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 22/80 (27%), Positives = 45/80 (56%), Gaps = 5/80 (6%)
 Frame = +1

Query: 10  LLTKMKSLVERIRLENTTLKKS-LDIERSRVRA-LKARYDSALRNLKTEHRKRE---EFL 174
           LL  +  L+ ++ L++++L  S   IE   V + L++R  +    +KT  +  +   EFL
Sbjct: 120 LLVGLIQLIRKLTLKDSSLSSSNFPIETEMVLSELESRISAVDGLVKTTTKMMQVQVEFL 179

Query: 175 EKQLRTIARPHKQTDDAQSA 234
           +K++ + +R  +QT D+ S+
Sbjct: 180 DKKMDSESRALRQTIDSTSS 199


>At3g05270.1 68416.m00575 expressed protein similar to
           endosome-associated protein (EEA1) (GI:1016368) [Homo
           sapiens]; similar to smooth muscle myosin heavy chain
           (GI:4417214) [Homo sapiens; contains Pfam profile
           PF05911: Plant protein of unknown function (DUF869)
          Length = 615

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 20/69 (28%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
 Frame = +1

Query: 4   ESLLTKMKSLVERIRLENTTLK-KSLDIERSRVRALKARYDSALRNLKTEHRKREEFLEK 180
           E++ +++K +      E+  LK KSL+    + RAL A+++S    L+ E  K ++ LE 
Sbjct: 502 EAIESRLKDV--EAEAESLILKIKSLEDVTEKERALSAKHNSKCNELQDEISKLKQELEH 559

Query: 181 QLRTIARPH 207
              T   P+
Sbjct: 560 HQETEPAPN 568


>At3g01810.1 68416.m00123 expressed protein
          Length = 921

 Score = 27.5 bits (58), Expect = 6.7
 Identities = 13/49 (26%), Positives = 21/49 (42%)
 Frame = +1

Query: 316 LRSHNRRSAHARLSSGPRRRDTRPWSKPHEGMHVRNAINCWRKSNRRSG 462
           L S NRR +  ++       D +PW        +R+ +  W   +R SG
Sbjct: 5   LSSKNRRCSSVQVDYLIHIHDIKPWPPSQSLRSLRSVVIQWENGDRNSG 53


>At3g22790.1 68416.m02873 kinase interacting family protein similar to
            kinase interacting protein 1 (GI:13936326) [Petunia
            integrifolia]
          Length = 1694

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
 Frame = +1

Query: 19   KMKSLVERIRLENTTLKKSLDIERSRVRALKARYDSALRNLKTE---HRKREEFLEKQLR 189
            K+K  +E+   E   L    D E   +  LK   +S ++ L  E   HR REEFL  +L+
Sbjct: 1202 KLKGNLEKRNSELCDLAGRQDEEIKILSNLKENLESEVKLLHKEIQEHRVREEFLSSELQ 1261


>At2g45290.1 68415.m05637 transketolase, putative strong similarity
           to transketolase 1 [Capsicum annuum] GI:3559814;
           contains Pfam profiles PF02779: Transketolase, pyridine
           binding domain, PF02780: Transketolase, C-terminal
           domain, PF00456: Transketolase, thiamine diphosphate
           binding domain
          Length = 741

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 17/61 (27%), Positives = 25/61 (40%)
 Frame = +2

Query: 365 PGGETRGLGASRTKGCTCAMP*TAGGNQIEGADNSTAETRGGKIDRATNDQAQRMEFGAN 544
           PG  TR L          A+P   GG+    + N T     G   +AT ++ + + FG  
Sbjct: 429 PGDATRNLSQQCLNALAKAVPGFLGGSADLASSNMTMLKAFGNFQKATPEE-RNLRFGVR 487

Query: 545 E 547
           E
Sbjct: 488 E 488


>At2g07020.1 68415.m00803 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 700

 Score = 27.1 bits (57), Expect = 8.8
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +3

Query: 426 HKLLEEIKSKERTIALLKREAAKSTA 503
           HKL E I +KE  +A+ + E AKS A
Sbjct: 332 HKLEEAILAKEAAMAIAENEKAKSRA 357


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,696,316
Number of Sequences: 28952
Number of extensions: 205728
Number of successful extensions: 794
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -