BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= pg--0710.Seq (766 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosacchar... 29 0.55 SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 28 1.7 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 2.9 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.1 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 6.8 SPBC2G5.06c |hmt2|cad1|sulfide-quinone oxidoreductase|Schizosacc... 25 9.0 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 25 9.0 >SPAC1786.01c ||SPAC31G5.20c|triacylglycerol lipase|Schizosaccharomyces pombe|chr 1|||Manual Length = 630 Score = 29.5 bits (63), Expect = 0.55 Identities = 17/46 (36%), Positives = 25/46 (54%) Frame = -2 Query: 333 SCSVPGMPLPVPPRTRQMTELKEP*QHNSIDSFHN*SLAGDFCISD 196 SC+VPG+ P+P TR + P HN + F + SL D +S+ Sbjct: 397 SCAVPGILNPIPLMTRSQSHRLIP--HNFGNRFKDGSLRTDIPLSE 440 >SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 27.9 bits (59), Expect = 1.7 Identities = 10/31 (32%), Positives = 15/31 (48%) Frame = +1 Query: 367 PPSMPWKRNWLRKPRQPVPVDTASLPPPTTI 459 PPS P W ++ P P +PPP ++ Sbjct: 1243 PPSPPSSSVWNKREHHPKPFSLHQVPPPESL 1273 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 27.1 bits (57), Expect = 2.9 Identities = 11/28 (39%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Frame = +1 Query: 379 PWKRNWLRKP-RQPVPVDTASLPPPTTI 459 P +NWLR+P +QP+ +LP P ++ Sbjct: 9 PNSKNWLRQPDQQPIQDSICTLPGPGSL 36 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 26.2 bits (55), Expect = 5.1 Identities = 10/33 (30%), Positives = 19/33 (57%) Frame = -2 Query: 426 HWHRLPRLSQPVSLPGHRGWMRHRQKPRRFYSC 328 +W R ++ VS P G++ +R + +R+ SC Sbjct: 150 YWERTSLMNVEVSAPEFPGFLHYRIQLKRYESC 182 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 25.8 bits (54), Expect = 6.8 Identities = 13/34 (38%), Positives = 20/34 (58%) Frame = +1 Query: 355 PMAHPPSMPWKRNWLRKPRQPVPVDTASLPPPTT 456 P++ P P R R PR+P+P + SL PP++ Sbjct: 44 PVSKKPLPPPTR---RLPRKPLPFRSTSLQPPSS 74 >SPBC2G5.06c |hmt2|cad1|sulfide-quinone oxidoreductase|Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 25.4 bits (53), Expect = 9.0 Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Frame = -3 Query: 509 AYPSYELLINYRSTA*-FIVVGGGSDAVSTGTGCRGFLSQFRFQGIEG 369 ++ S +L + ST +VVGGGS +S S++RF +G Sbjct: 14 SFQSASMLARFASTHHKVLVVGGGSAGISVAHQIYNKFSKYRFANDQG 61 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 25.4 bits (53), Expect = 9.0 Identities = 20/78 (25%), Positives = 31/78 (39%) Frame = +3 Query: 93 Q*VRYQSIYLNKAVNSLRFYLS*KNVILITSTKQRHLYKNHLREINYENYQYCCAAMALS 272 Q + + S+ +K+V L Y N L + LY E YCCA + S Sbjct: 559 QDIEFGSLDADKSVTVLFSYDGKLNGALDAHFQTAVLYTTPSGERRVRVVNYCCAVTSKS 618 Query: 273 TLSFGVFAAEPVTASQAQ 326 + + EP+ A A+ Sbjct: 619 YDTISLAIVEPIVAVLAK 636 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,056,250 Number of Sequences: 5004 Number of extensions: 62462 Number of successful extensions: 137 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 367316502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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