Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0704.Seq
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 39 8e-04
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 37 0.003
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 36 0.004
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 33 0.031
SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch... 29 0.51
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 29 0.67
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 2.7
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 27 3.6
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 26 4.7
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 25 8.3
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 38.7 bits (86), Expect = 8e-04
Identities = 16/56 (28%), Positives = 32/56 (57%)
Frame = +2
Query: 344 LHLVFPENFPFAPPFMRVIEPRIDKGFVMEGGAICMELLTPRGWASAYTVEAVVMS 511
+ + FP N+P++PP + P M G IC+++L + W++ Y V+ +++S
Sbjct: 80 ISMSFPANYPYSPPTIIFTSPMWHPNVDMSGN-ICLDILKDK-WSAVYNVQTILLS 133
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +2
Query: 344 LHLVFPENFPFAPPFMRVIEPRIDKGFVMEGGAICMELLT-PRGWASAYTVEAVVM 508
L + FPE +P PP R P V G +C+ +L GW A T++ +++
Sbjct: 60 LTMAFPEEYPTRPPKCRFTPPLFHPN-VYPSGTVCLSILNEEEGWKPAITIKQILL 114
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 36.3 bits (80), Expect = 0.004
Identities = 18/56 (32%), Positives = 32/56 (57%)
Frame = +2
Query: 344 LHLVFPENFPFAPPFMRVIEPRIDKGFVMEGGAICMELLTPRGWASAYTVEAVVMS 511
L + FP ++PF PP + RI + G+IC+++L + W+ A T+ V++S
Sbjct: 52 LSIHFPTDYPFKPPKVN-FTTRIYHPNINSNGSICLDILRDQ-WSPALTISKVLLS 105
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 33.5 bits (73), Expect = 0.031
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 350 LVFPENFPFAPPFMRVIEPRIDKGFVMEGGAICMEL--LTPRGWASAYTVEAVVM 508
L+FP ++PF PP +R+I P G +C+ P+ W ++ V +++
Sbjct: 58 LIFPPDYPFKPPAIRMITP---SGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILV 109
>SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 658
Score = 29.5 bits (63), Expect = 0.51
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = -1
Query: 508 HHHRLHGVRGRPAARRQQLHAYRSTFHNESFIYSRFDDTHKRRC 377
HH ++ G R R A+ +LH Y + NE+F+ S ++ +C
Sbjct: 40 HHEKVTGCRPRQASS-DELHKYSKSASNEAFLSSFAENQLFHKC 82
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 29.1 bits (62), Expect = 0.67
Identities = 23/75 (30%), Positives = 33/75 (44%)
Frame = +2
Query: 248 RQFFEWHVRLHQIDPESDLATDLRELHIPNILLHLVFPENFPFAPPFMRVIEPRIDKGFV 427
+ FEW V + I PE D + H L FP+++P PP M+ I V
Sbjct: 33 KSIFEWEVMI--IGPE-DTLYEGGFFHAT-----LSFPQDYPLMPPKMK-FTTEIWHPNV 83
Query: 428 MEGGAICMELLTPRG 472
G +C+ +L P G
Sbjct: 84 HPNGEVCISILHPPG 98
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 27.1 bits (57), Expect = 2.7
Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +3
Query: 237 ELVNDNFSSGT*GCTRSIQRV-ILPRTYENCTFRTSSYT*CSQRIFHLHRRLCVSSNRE 410
E+ +D+ S + CT ++ + +LP T+ N +SSY H+ L + +RE
Sbjct: 108 EICHDDCLSSSPPCTSALTEITLLPPTFHNLIPSSSSYETAVAEFLHMEDLLQENVSRE 166
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 26.6 bits (56), Expect = 3.6
Identities = 10/50 (20%), Positives = 25/50 (50%)
Frame = +2
Query: 362 ENFPFAPPFMRVIEPRIDKGFVMEGGAICMELLTPRGWASAYTVEAVVMS 511
E +P +PP + + G +CM++L W+ A+++++ ++
Sbjct: 62 EGYPISPPSVYFQTKIVHPNISWTNGEVCMDILKTH-WSPAWSLQSACLA 110
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 26.2 bits (55), Expect = 4.7
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = +2
Query: 293 ESDLATDLRELHIPNILLHLVFPENFPFAPP 385
ES L + H P++++ PE +PF P
Sbjct: 57 ESTLTDTIVVRHFPDLVMEFFLPEAYPFNSP 87
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +2
Query: 350 LVFPENFPFAPPFMRVIEPRIDKGFVMEGGAICMELLTPRG 472
L FP ++P PP ++ E V + G +C+ +L G
Sbjct: 59 LKFPSDYPLGPPTLK-FECEFFHPNVYKDGTVCISILHAPG 98
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,895,164
Number of Sequences: 5004
Number of extensions: 57627
Number of successful extensions: 175
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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