Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0691.Seq
(588 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putativ... 97 6e-21
At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putativ... 93 9e-20
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 38 0.005
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 38 0.005
At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein... 37 0.011
At5g18570.1 68418.m02195 GTP1/OBG family protein similar to SP|P... 34 0.061
At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein... 33 0.11
At1g70180.2 68414.m08076 sterile alpha motif (SAM) domain-contai... 33 0.11
At1g70180.1 68414.m08075 sterile alpha motif (SAM) domain-contai... 33 0.11
At5g51660.1 68418.m06405 cleavage and polyadenylation specificit... 33 0.19
At5g18180.1 68418.m02134 Gar1 RNA-binding region family protein ... 32 0.25
At1g16610.2 68414.m01990 arginine/serine-rich protein, putative ... 31 0.43
At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to SP... 31 0.57
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 30 1.00
At5g46270.1 68418.m05696 disease resistance protein (TIR-NBS-LRR... 29 1.7
At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 29 2.3
At1g16610.1 68414.m01989 arginine/serine-rich protein, putative ... 29 2.3
At5g18350.1 68418.m02159 disease resistance protein (TIR-NBS-LRR... 28 4.0
>At4g05420.1 68417.m00824 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa]; contains Pfam PF03178 : CPSF A subunit
region
Length = 1088
Score = 97.5 bits (232), Expect = 6e-21
Identities = 47/87 (54%), Positives = 63/87 (72%), Gaps = 4/87 (4%)
Frame = +3
Query: 3 KLVQVAEKEIKGGCYSLVEFNGKLLAAINSMVRLFEWT----SDKELRLECSHFNNIVAL 170
+L +AEKE KG YSL FNGKLLAAIN ++L++W +EL+ EC H +I+AL
Sbjct: 812 RLQLIAEKETKGAVYSLNAFNGKLLAAINQKIQLYKWMLRDDGTRELQSECGHHGHILAL 871
Query: 171 YLKAKGDFILVGDLMRSMSLLQYKQME 251
Y++ +GDFI+VGDLM+S+SLL YK E
Sbjct: 872 YVQTRGDFIVVGDLMKSISLLLYKHEE 898
Score = 96.3 bits (229), Expect = 1e-20
Identities = 43/74 (58%), Positives = 55/74 (74%)
Frame = +2
Query: 251 GRFEEIARDYSPNWMTAVEILDDDTFLGAENSFNLFVCQKDSAATTDEDRQQMGYAGQFH 430
G EE ARDY+ NWM+AVEILDDD +LGAEN+FNL +K+S TDE+R ++ G++H
Sbjct: 899 GAIEERARDYNANWMSAVEILDDDIYLGAENNFNLLTVKKNSEGATDEERGRLEVVGEYH 958
Query: 431 LGDMVNVMRAGSLV 472
LG+ VN R GSLV
Sbjct: 959 LGEFVNRFRHGSLV 972
>At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa] and damage-specific DNA binding protein 1,
Homo sapiens, PIR2:I38908; contains Pfam PF03178 : CPSF A
subunit region
Length = 1088
Score = 93.5 bits (222), Expect = 9e-20
Identities = 45/87 (51%), Positives = 61/87 (70%), Gaps = 4/87 (4%)
Frame = +3
Query: 3 KLVQVAEKEIKGGCYSLVEFNGKLLAAINSMVRLFEWT----SDKELRLECSHFNNIVAL 170
+L + EKE KG YSL FNGKLLA+IN ++L++W +EL+ EC H +I+AL
Sbjct: 812 RLQLITEKETKGAVYSLNAFNGKLLASINQKIQLYKWMLRDDGTRELQSECGHHGHILAL 871
Query: 171 YLKAKGDFILVGDLMRSMSLLQYKQME 251
Y++ +GDFI VGDLM+S+SLL YK E
Sbjct: 872 YVQTRGDFIAVGDLMKSISLLIYKHEE 898
Score = 93.5 bits (222), Expect = 9e-20
Identities = 40/74 (54%), Positives = 54/74 (72%)
Frame = +2
Query: 251 GRFEEIARDYSPNWMTAVEILDDDTFLGAENSFNLFVCQKDSAATTDEDRQQMGYAGQFH 430
G EE ARDY+ NWMTAVEIL+DD +LG +N FN+F +K++ TDE+R +M G++H
Sbjct: 899 GAIEERARDYNANWMTAVEILNDDIYLGTDNCFNIFTVKKNNEGATDEERARMEVVGEYH 958
Query: 431 LGDMVNVMRAGSLV 472
+G+ VN R GSLV
Sbjct: 959 IGEFVNRFRHGSLV 972
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 37.9 bits (84), Expect = 0.005
Identities = 23/76 (30%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
Frame = +3
Query: 27 EIKGGCYSLVEFNGKLLAAINSMVRLFEWTSDKELR-LECSHFNNIVALYLKAKGDFILV 203
+++G +L +F G+LLA I ++RL++ + LR E F N + + ++ D I V
Sbjct: 942 QVEGVPLALCQFQGRLLAGIGPVLRLYDLGKKRLLRKCENKLFPNTI-ISIQTYRDRIYV 1000
Query: 204 GDLMRSMSLLQYKQME 251
GD+ S +Y++ E
Sbjct: 1001 GDIQESFHYCKYRRDE 1016
Score = 29.5 bits (63), Expect = 1.7
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 18/86 (20%)
Frame = +2
Query: 269 ARDYSPNWMTAVEILDDDTFLGAENSFNLFVCQ--KDSAATTDED------RQQMGYAG- 421
A D P W+TA +D DT GA+ N++ + +D + +ED + + G
Sbjct: 1023 ADDCVPRWLTASHHVDFDTMAGADKFGNVYFVRLPQDLSEEIEEDPTGGKIKWEQGKLNG 1082
Query: 422 ---------QFHLGDMVNVMRAGSLV 472
QFH+GD+V ++ S++
Sbjct: 1083 APNKVDEIVQFHVGDVVTCLQKASMI 1108
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 37.9 bits (84), Expect = 0.005
Identities = 23/76 (30%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
Frame = +3
Query: 27 EIKGGCYSLVEFNGKLLAAINSMVRLFEWTSDKELR-LECSHFNNIVALYLKAKGDFILV 203
+++G +L +F G+LLA I ++RL++ + LR E F N + + ++ D I V
Sbjct: 942 QVEGVPLALCQFQGRLLAGIGPVLRLYDLGKKRLLRKCENKLFPNTI-ISIQTYRDRIYV 1000
Query: 204 GDLMRSMSLLQYKQME 251
GD+ S +Y++ E
Sbjct: 1001 GDIQESFHYCKYRRDE 1016
Score = 29.5 bits (63), Expect = 1.7
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 18/86 (20%)
Frame = +2
Query: 269 ARDYSPNWMTAVEILDDDTFLGAENSFNLFVCQ--KDSAATTDED------RQQMGYAG- 421
A D P W+TA +D DT GA+ N++ + +D + +ED + + G
Sbjct: 1023 ADDCVPRWLTASHHVDFDTMAGADKFGNVYFVRLPQDLSEEIEEDPTGGKIKWEQGKLNG 1082
Query: 422 ---------QFHLGDMVNVMRAGSLV 472
QFH+GD+V ++ S++
Sbjct: 1083 APNKVDEIVQFHVGDVVTCLQKASMI 1108
>At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein
(ZWICHEL) identical to kinesin-like protein GI:2224925
from [Arabidopsis thaliana]
Length = 1260
Score = 36.7 bits (81), Expect = 0.011
Identities = 33/113 (29%), Positives = 56/113 (49%), Gaps = 8/113 (7%)
Frame = +2
Query: 146 SLQQHRGSVPEGERRLHPGRGS-DAL----HVATAI*TDGGRFEEIARDYSPNWMTAVEI 310
+L+ + S+ G R PGR +AL + T + FEEI+ D + AVE
Sbjct: 248 TLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVEE 307
Query: 311 LDDDTFLGAENSFNLFVCQK--DSAATTDEDRQQ-MGYAGQFHLGDMVNVMRA 460
L L A +SF+LF C+K S+ ++D ++ +G ++GD++ +A
Sbjct: 308 LAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKA 360
>At5g18570.1 68418.m02195 GTP1/OBG family protein similar to
SP|P20964 Spo0B-associated GTP-binding protein {Bacillus
subtilis}; contains Pfam profile PF01018: GTP1/OBG
family
Length = 681
Score = 34.3 bits (75), Expect = 0.061
Identities = 19/46 (41%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Frame = +1
Query: 292 DDGRGDSGRRHVSGRREQLQPFRLS---KGQRGHHGRGPAADGLRG 420
D GRG + V G L PFR S + RG HGRG G +G
Sbjct: 245 DGGRGGNVYVEVDGSMNSLLPFRKSVHFRAGRGEHGRGKMQSGAKG 290
>At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein
(ZWICHEL) identical to kinesin-like protein GI:2224925
from [Arabidopsis thaliana]
Length = 1259
Score = 33.5 bits (73), Expect = 0.11
Identities = 33/113 (29%), Positives = 56/113 (49%), Gaps = 8/113 (7%)
Frame = +2
Query: 146 SLQQHRGSVPEGERRLHPGRGS-DAL----HVATAI*TDGGRFEEIARDYSPNWMTAVEI 310
+L+ + S+ G R PGR +AL + T + FEEI+ D + AVE
Sbjct: 248 TLKALKRSIKAGPRHTTPGREEIEALLTGRKLTTIVFFLDETFEEISYDMATTVSDAVE- 306
Query: 311 LDDDTFLGAENSFNLFVCQK--DSAATTDEDRQQ-MGYAGQFHLGDMVNVMRA 460
L L A +SF+LF C+K S+ ++D ++ +G ++GD++ +A
Sbjct: 307 LAGTIKLSAFSSFSLFECRKVVSSSKSSDPGNEEYIGLDDNKYIGDLLAEFKA 359
>At1g70180.2 68414.m08076 sterile alpha motif (SAM)
domain-containing protein contains Pfam profile PF00536:
SAM domain (Sterile alpha motif)
Length = 460
Score = 33.5 bits (73), Expect = 0.11
Identities = 25/83 (30%), Positives = 32/83 (38%)
Frame = +1
Query: 250 RALRGDSPRLQPQLDDGRGDSGRRHVSGRREQLQPFRLSKGQRGHHGRGPAADGLRGAVP 429
R + G + P DGR R +S R+ + F + Q G R P
Sbjct: 200 RRMIGSARDRSPPRSDGRIIGSPRDISPPRDAGRRFGPPRDQSPPRNAGRVTGSPRDRSP 259
Query: 430 PRRHGQRHARRLPRGASPPRRLR 498
PR G+R PR SPPR R
Sbjct: 260 PRNAGRRMGP--PRDQSPPRSTR 280
>At1g70180.1 68414.m08075 sterile alpha motif (SAM)
domain-containing protein contains Pfam profile PF00536:
SAM domain (Sterile alpha motif)
Length = 456
Score = 33.5 bits (73), Expect = 0.11
Identities = 25/83 (30%), Positives = 32/83 (38%)
Frame = +1
Query: 250 RALRGDSPRLQPQLDDGRGDSGRRHVSGRREQLQPFRLSKGQRGHHGRGPAADGLRGAVP 429
R + G + P DGR R +S R+ + F + Q G R P
Sbjct: 200 RRMIGSARDRSPPRSDGRIIGSPRDISPPRDAGRRFGPPRDQSPPRNAGRVTGSPRDRSP 259
Query: 430 PRRHGQRHARRLPRGASPPRRLR 498
PR G+R PR SPPR R
Sbjct: 260 PRNAGRRMGP--PRDQSPPRSTR 280
>At5g51660.1 68418.m06405 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to SP|Q9EPU4 Cleavage and polyadenylation
specificity factor, 160 kDa subunit (CPSF 160 kDa
subunit) {Mus musculus}; contains Pfam profile PF03178:
CPSF A subunit region
Length = 1442
Score = 32.7 bits (71), Expect = 0.19
Identities = 17/80 (21%), Positives = 37/80 (46%)
Frame = +3
Query: 6 LVQVAEKEIKGGCYSLVEFNGKLLAAINSMVRLFEWTSDKELRLECSHFNNIVALYLKAK 185
+ +V +E+KG ++ G LL + + L +W + + + + +
Sbjct: 1165 VTEVYSRELKGAISAVASIQGHLLISSGPKIILHKWNGTELNGVAFFDAPPLYVVSMNVV 1224
Query: 186 GDFILVGDLMRSMSLLQYKQ 245
FIL+GD+ +S+ L +K+
Sbjct: 1225 KSFILLGDVHKSIYFLSWKE 1244
>At5g18180.1 68418.m02134 Gar1 RNA-binding region family protein
contains Pfam profile PF04410: Gar1 protein RNA binding
region
Length = 189
Score = 32.3 bits (70), Expect = 0.25
Identities = 19/38 (50%), Positives = 19/38 (50%)
Frame = +1
Query: 367 KGQRGHHGRGPAADGLRGAVPPRRHGQRHARRLPRGAS 480
KGQ G GRG RG PPR G R PRGAS
Sbjct: 135 KGQSG--GRGEGRVPPRGRGPPRGRGNFRGRGAPRGAS 170
>At1g16610.2 68414.m01990 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 407
Score = 31.5 bits (68), Expect = 0.43
Identities = 30/91 (32%), Positives = 38/91 (41%), Gaps = 9/91 (9%)
Frame = +1
Query: 253 ALRGDSPRLQPQLDDGRGDSG----RRHVSGRREQLQPFR-LSKGQRGH---HGRGPAAD 408
A + D+P+ D D G R +S RR P R LS +R H R P +
Sbjct: 191 APKRDAPKSDNAAADAEKDGGPRRPRERLSPRRRSPLPRRGLSPRRRSPDSPHRRRPGSP 250
Query: 409 -GLRGAVPPRRHGQRHARRLPRGASPPRRLR 498
RG PPRR +R + PPRR R
Sbjct: 251 IRRRGDTPPRRRPASPSRGRSPSSPPPRRYR 281
>At1g71270.1 68414.m08225 Vps52/Sac2 family protein similar to
SP|P39904 SAC2 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04129: Vps52 / Sac2 family
Length = 707
Score = 31.1 bits (67), Expect = 0.57
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +2
Query: 260 EEIARDYSPNWMTAVEILDDDTFLGAENSFNLFVCQKD 373
E + +D+ W TA+E++D D SF+ F+C D
Sbjct: 624 EPLVKDFGSRWKTAIELMDKDII----TSFSNFLCGMD 657
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 30.3 bits (65), Expect = 1.00
Identities = 19/48 (39%), Positives = 20/48 (41%), Gaps = 1/48 (2%)
Frame = +1
Query: 376 RGHHGRGPAADGLRGAVPP-RRHGQRHARRLPRGASPPRRLRRAHDRP 516
R H P A R PP RRH R R SPP R RR+ P
Sbjct: 338 RRHRSPTPPARQRRSPSPPARRHRSPPPARRRRSPSPPARRRRSPSPP 385
>At5g46270.1 68418.m05696 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1145
Score = 29.5 bits (63), Expect = 1.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -3
Query: 457 AHDVDHVAEVELPRVAHLLPVL 392
AH++DH+ EV LP H L +L
Sbjct: 335 AHEIDHIYEVSLPTQQHALAML 356
>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
similarity to SP|P12036 Neurofilament triplet H protein
{Homo sapiens}; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 636
Score = 29.1 bits (62), Expect = 2.3
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = -3
Query: 355 KVEAVLCAQK-RVVVQNLHGRHPVGAVVAGYLLEAPSICLY-CSSDMERIRSPTRMK 191
KV+A L AQ+ R QNL G V AV+ G+L+ ++ Y C + ++++ R K
Sbjct: 112 KVQAALRAQQAREESQNLKGITRVQAVIRGHLVRRQAVATYSCIWGIVKVQALVRGK 168
>At1g16610.1 68414.m01989 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 414
Score = 29.1 bits (62), Expect = 2.3
Identities = 23/65 (35%), Positives = 26/65 (40%)
Frame = +1
Query: 304 GDSGRRHVSGRREQLQPFRLSKGQRGHHGRGPAADGLRGAVPPRRHGQRHARRLPRGASP 483
G S RR R L P R S H R + RG PPRR +R + P
Sbjct: 225 GLSPRRRSPLPRRGLSPRRRSPDSP-HRRRPGSPIRRRGDTPPRRRPASPSRGRSPSSPP 283
Query: 484 PRRLR 498
PRR R
Sbjct: 284 PRRYR 288
>At5g18350.1 68418.m02159 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1193
Score = 28.3 bits (60), Expect = 4.0
Identities = 19/71 (26%), Positives = 31/71 (43%)
Frame = -3
Query: 457 AHDVDHVAEVELPRVAHLLPVLVRGGRAVLLTNEKVEAVLCAQKRVVVQNLHGRHPVGAV 278
AH++D++ EV LPR L + ++ + + C V L G P+G
Sbjct: 353 AHNIDYIYEVGLPRKDDALQIFCLSAFGQNFPHDDFQYLACE-----VTQLAGELPLGLK 407
Query: 277 VAGYLLEAPSI 245
V G L+ S+
Sbjct: 408 VLGSYLKGMSL 418
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,192,255
Number of Sequences: 28952
Number of extensions: 253402
Number of successful extensions: 843
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 795
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 838
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -