Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0689.Seq
(772 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 147 8e-36
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 147 8e-36
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 146 1e-35
At1g68030.1 68414.m07772 PHD finger protein-related contains low... 33 0.28
At2g42100.1 68415.m05205 actin, putative very strong similarity ... 31 1.1
At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to S... 29 2.6
At2g46980.2 68415.m05869 expressed protein 29 2.6
At2g46980.1 68415.m05868 expressed protein 29 2.6
At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496... 29 3.4
At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497... 29 4.5
At1g71300.1 68414.m08228 Vps52/Sac2 family protein similar to SP... 29 4.5
At4g40050.1 68417.m05671 expressed protein 28 5.9
At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493 A... 28 5.9
At2g39750.1 68415.m04881 dehydration-responsive family protein s... 28 5.9
At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671 A... 28 5.9
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 7.9
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 28 7.9
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 147 bits (356), Expect = 8e-36
Identities = 66/88 (75%), Positives = 73/88 (82%)
Frame = +1
Query: 250 KEYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGW 429
K+YNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV GW
Sbjct: 67 KKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVPGW 126
Query: 430 DKPIIIGRHAHADQYKATDFVVPGAGIL 513
KPI IGRHA DQY+ATD V+ G G L
Sbjct: 127 TKPICIGRHAFGDQYRATDAVIKGPGKL 154
Score = 92.7 bits (220), Expect = 2e-19
Identities = 44/85 (51%), Positives = 59/85 (69%)
Frame = +3
Query: 510 LEIIFKPESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKN 689
L + F+ + G+ V GVA+AM+NTD SI FA +S A ++K+PLYLSTKN
Sbjct: 154 LTMTFEGKDGKTETEVFTFTGEGGVAMAMYNTDESIRAFADASMNTAYEKKWPLYLSTKN 213
Query: 690 TILKKYDGRFKDIFQDIYDREYKKR 764
TILKKYDGRFKDIFQ++Y+ +K +
Sbjct: 214 TILKKYDGRFKDIFQEVYEASWKSK 238
Score = 81.0 bits (191), Expect = 8e-16
Identities = 36/63 (57%), Positives = 51/63 (80%), Gaps = 1/63 (1%)
Frame = +2
Query: 68 KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELNVYDLGMENRDKTDDQVTIDCAE 244
KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE
Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64
Query: 245 AIK 253
A K
Sbjct: 65 ATK 67
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 147 bits (356), Expect = 8e-36
Identities = 67/88 (76%), Positives = 72/88 (81%)
Frame = +1
Query: 253 EYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWD 432
+YNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV GW
Sbjct: 68 KYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVPGWT 127
Query: 433 KPIIIGRHAHADQYKATDFVVPGAGILK 516
KPI IGRHA DQY+ATD +V G LK
Sbjct: 128 KPICIGRHAFGDQYRATDLIVNEPGKLK 155
Score = 97.9 bits (233), Expect = 6e-21
Identities = 46/86 (53%), Positives = 62/86 (72%), Gaps = 1/86 (1%)
Frame = +3
Query: 510 LEIIFKPE-SGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTK 686
L+++F+P S + + V + G GVALAM+NTD SI FA SS A +K+PLYLSTK
Sbjct: 154 LKLVFEPSGSSQKTEFEVFNFTGGGVALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTK 213
Query: 687 NTILKKYDGRFKDIFQDIYDREYKKR 764
NTILK YDGRFKDIFQ++Y+ ++ +
Sbjct: 214 NTILKIYDGRFKDIFQEVYEANWRSK 239
Score = 81.8 bits (193), Expect = 5e-16
Identities = 37/61 (60%), Positives = 49/61 (80%), Gaps = 1/61 (1%)
Frame = +2
Query: 68 KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELNVYDLGMENRDKTDDQVTIDCAE 244
KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE
Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64
Query: 245 A 247
A
Sbjct: 65 A 65
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 146 bits (355), Expect = 1e-35
Identities = 66/88 (75%), Positives = 73/88 (82%)
Frame = +1
Query: 253 EYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWD 432
+YNV IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+
Sbjct: 138 KYNVAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWE 197
Query: 433 KPIIIGRHAHADQYKATDFVVPGAGILK 516
KPI IGRHA DQY+ATD V+ G G LK
Sbjct: 198 KPICIGRHAFGDQYRATDTVIKGPGKLK 225
Score = 110 bits (264), Expect = 1e-24
Identities = 51/86 (59%), Positives = 68/86 (79%), Gaps = 1/86 (1%)
Frame = +3
Query: 510 LEIIFKPESGEA-IKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTK 686
L+++F PE G A ++ V+++KG GVALAM+N D SI FA SS AL +K+PLYLSTK
Sbjct: 224 LKMVFVPEDGNAPVELDVYDFKGPGVALAMYNVDESIRAFAESSMAMALTKKWPLYLSTK 283
Query: 687 NTILKKYDGRFKDIFQDIYDREYKKR 764
NTILKKYDGRFKDIFQ++Y+ +K++
Sbjct: 284 NTILKKYDGRFKDIFQEVYEANWKQK 309
Score = 83.8 bits (198), Expect = 1e-16
Identities = 34/55 (61%), Positives = 48/55 (87%)
Frame = +2
Query: 83 PVVDILGDEMTRIIWDLIKEKLILPFLDIELNVYDLGMENRDKTDDQVTIDCAEA 247
P+V++ GDEMTR+IW +IKEKLILP+LD+++ +DLG+ NRD TDD+VT++ AEA
Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEA 135
>At1g68030.1 68414.m07772 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 314
Score = 32.7 bits (71), Expect = 0.28
Identities = 17/36 (47%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Frame = -1
Query: 133 KIPDYSGHFITKDVNNR--SSLYFRHIVSVTGVTTT 32
K+PDY G + + V R +SL+ H+VSVT TTT
Sbjct: 49 KLPDYLGEYTNEMVAFRCLASLFDSHVVSVTTTTTT 84
>At2g42100.1 68415.m05205 actin, putative very strong similarity to
SP|P53496 Actin 11 {Arabidopsis thaliana}, SP|P53493
Actin 3 {Arabidopsis thaliana}; contains Pfam profile
PF00022: Actin
Length = 378
Score = 30.7 bits (66), Expect = 1.1
Identities = 19/77 (24%), Positives = 35/77 (45%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D K ++R Y S + I++
Sbjct: 157 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTEYLTKIMMERGYTYTTSAEREIVRDIK 216
Query: 711 GRFKDIFQDIYDREYKK 761
+ I D Y++E +K
Sbjct: 217 EKLCYIAVD-YEQEMEK 232
>At5g09810.1 68418.m01135 actin 7 (ACT7) / actin 2 identical to
SP|P53492 Actin 7 (Actin-2) {Arabidopsis thaliana}
Length = 377
Score = 29.5 bits (63), Expect = 2.6
Identities = 17/74 (22%), Positives = 33/74 (44%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D S K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYMFTTTAEREIVRDIK 215
Query: 711 GRFKDIFQDIYDRE 752
+ + D Y++E
Sbjct: 216 EKLAYVALD-YEQE 228
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.6
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 468 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 355
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 2.6
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 468 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 355
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At3g12110.1 68416.m01507 actin 11 (ACT11) identical to SP|P53496
Actin 11 {Arabidopsis thaliana}
Length = 377
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/74 (24%), Positives = 32/74 (43%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D K +R Y S + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTSAEREIVRDVK 215
Query: 711 GRFKDIFQDIYDRE 752
+ I D Y++E
Sbjct: 216 EKLAYIALD-YEQE 228
>At3g46520.1 68416.m05050 actin 12 (ACT12) identical to SP|P53497
Actin 12 {Arabidopsis thaliana}
Length = 377
Score = 28.7 bits (61), Expect = 4.5
Identities = 17/74 (22%), Positives = 32/74 (43%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDHLMKILTERGYSFTTTAEREIVRDMK 215
Query: 711 GRFKDIFQDIYDRE 752
+ I D Y++E
Sbjct: 216 EKLSYIALD-YEQE 228
>At1g71300.1 68414.m08228 Vps52/Sac2 family protein similar to
SP|P39904 SAC2 protein {Saccharomyces cerevisiae};
contains Pfam profile PF04129: Vps52 / Sac2 family
Length = 701
Score = 28.7 bits (61), Expect = 4.5
Identities = 23/67 (34%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = +2
Query: 110 MTRIIW--DLIKEKLILPFLDIELNVYDLGMENRDKTDDQVTI-DCAEAIKNTMWASNVR 280
M RII LI + +P LD L+ ++ + R KT + I A NT+W +V
Sbjct: 426 MIRIIHHHQLIMSRRRIPCLDSYLDKVNISLWPRFKTVFDLHIGSLRNANINTIWEDDVH 485
Query: 281 P*LLMRR 301
P +MRR
Sbjct: 486 PHYIMRR 492
>At4g40050.1 68417.m05671 expressed protein
Length = 599
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = -2
Query: 474 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 325
VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596
>At3g53750.1 68416.m05938 actin 3 (ACT3) identical to SP|P53493
Actin 3 {Arabidopsis thaliana}; supported by full-length
cDNA: Ceres: 19581.
Length = 377
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/74 (22%), Positives = 33/74 (44%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D + K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDIK 215
Query: 711 GRFKDIFQDIYDRE 752
+ I D Y++E
Sbjct: 216 EKLCYIALD-YEQE 228
>At2g39750.1 68415.m04881 dehydration-responsive family protein
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 694
Score = 28.3 bits (60), Expect = 5.9
Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
Frame = +3
Query: 501 CWYLEIIFKPESGEAIKHVVHEYKGAGVA---LAMFNTDASIIDFAHSSFKFALDRK 662
CW L ++ P SG V+++ GV F+T DF H+S F+++RK
Sbjct: 571 CWVLSVV--PVSGPNTLPVIYDRGLLGVMHDWCEPFDTYPRTYDFLHASGLFSIERK 625
>At2g37620.1 68415.m04615 actin 1 (ACT1) identical to SP|P10671
Actin 1 (Actin 3) {Arabidopsis thaliana}
Length = 377
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/74 (22%), Positives = 33/74 (44%)
Frame = +3
Query: 531 ESGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYD 710
+SG+ + H V Y+G + A+ D + D + K +R Y + + I++
Sbjct: 156 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYSFTTTAEREIVRDIK 215
Query: 711 GRFKDIFQDIYDRE 752
+ I D Y++E
Sbjct: 216 EKLCYIALD-YEQE 228
>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
Length = 160
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +3
Query: 387 GYYLQEYTETCNRVGQTHHHWTSCSC 464
G+YL E++ TC +V HH C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147
>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 512
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3
Query: 383 LKTVPPRILRMVPLGLFH 330
LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,757,380
Number of Sequences: 28952
Number of extensions: 359291
Number of successful extensions: 930
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 930
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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