Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0687.Seq
(773 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 1e-44
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 103 2e-23
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 100 2e-22
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 100 5e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 69 6e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 7e-12
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 59 8e-10
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 57 2e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 47 3e-06
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 36 0.006
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.32
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.9
SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2 |Schizosa... 27 3.9
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 5.2
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 5.2
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 9.1
SPAC1002.02 |mug31||nucleoporin Pom34 |Schizosaccharomyces pombe... 25 9.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 174 bits (423), Expect = 1e-44
Identities = 77/86 (89%), Positives = 82/86 (95%)
Frame = +3
Query: 252 EYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENF 431
+YPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FE F
Sbjct: 68 KYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETF 127
Query: 432 NSPAMYVAIQAVLSLYASGRTTGIVL 509
N+PA YVAIQAVLSLYASGRTTGIVL
Sbjct: 128 NAPAFYVAIQAVLSLYASGRTTGIVL 153
Score = 155 bits (377), Expect = 5e-39
Identities = 72/81 (88%), Positives = 78/81 (96%)
Frame = +2
Query: 509 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIK 688
DSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIK
Sbjct: 154 DSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIK 213
Query: 689 EKLCYVALDFEQEMATAAAST 751
EKLCYVALDFEQE+ TAA S+
Sbjct: 214 EKLCYVALDFEQELQTAAQSS 234
Score = 135 bits (327), Expect = 6e-33
Identities = 60/69 (86%), Positives = 66/69 (95%)
Frame = +1
Query: 55 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 234
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 235 RGILTLNTP 261
RGILTL P
Sbjct: 62 RGILTLKYP 70
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 103 bits (248), Expect = 2e-23
Identities = 43/86 (50%), Positives = 66/86 (76%)
Frame = +3
Query: 252 EYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENF 431
+YP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE +
Sbjct: 68 KYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERY 127
Query: 432 NSPAMYVAIQAVLSLYASGRTTGIVL 509
+YVAIQAVLSLYA G ++G+V+
Sbjct: 128 GFGGVYVAIQAVLSLYAQGLSSGVVV 153
Score = 94.7 bits (225), Expect = 1e-20
Identities = 42/81 (51%), Positives = 57/81 (70%)
Frame = +2
Query: 509 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIK 688
DSGDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++K
Sbjct: 154 DSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMK 213
Query: 689 EKLCYVALDFEQEMATAAAST 751
EKLCYV+ D E + + +T
Sbjct: 214 EKLCYVSYDLELDHKLSEETT 234
Score = 58.8 bits (136), Expect = 8e-10
Identities = 31/67 (46%), Positives = 42/67 (62%), Gaps = 2/67 (2%)
Frame = +1
Query: 67 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 240
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 241 ILTLNTP 261
+L + P
Sbjct: 64 LLQVKYP 70
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 100 bits (240), Expect = 2e-22
Identities = 43/78 (55%), Positives = 54/78 (69%)
Frame = +3
Query: 252 EYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENF 431
+YPI HG I NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+F
Sbjct: 91 DYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESF 150
Query: 432 NSPAMYVAIQAVLSLYAS 485
N +Y+A+QAVL+L AS
Sbjct: 151 NCAGLYIAVQAVLALAAS 168
Score = 61.3 bits (142), Expect = 1e-10
Identities = 33/77 (42%), Positives = 47/77 (61%), Gaps = 2/77 (2%)
Frame = +2
Query: 509 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRD 682
DSGDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER
Sbjct: 185 DSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER----- 239
Query: 683 IKEKLCYVALDFEQEMA 733
IKE+ CYV D +E +
Sbjct: 240 IKEECCYVCPDIVKEFS 256
Score = 42.7 bits (96), Expect = 6e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 73 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 222
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 99.5 bits (237), Expect = 5e-22
Identities = 46/85 (54%), Positives = 60/85 (70%), Gaps = 1/85 (1%)
Frame = +3
Query: 258 PIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFN 434
PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +E N
Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLN 133
Query: 435 SPAMYVAIQAVLSLYASGRTTGIVL 509
PA+ ++Q VL+LYAS RTTGIVL
Sbjct: 134 VPALSFSLQPVLALYASARTTGIVL 158
Score = 91.9 bits (218), Expect = 9e-20
Identities = 39/82 (47%), Positives = 59/82 (71%)
Frame = +2
Query: 509 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIK 688
+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IK
Sbjct: 159 ECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIK 218
Query: 689 EKLCYVALDFEQEMATAAASTP 754
EK CYVA DF E+ + P
Sbjct: 219 EKCCYVASDFRSEIESWTEHKP 240
Score = 79.0 bits (186), Expect = 7e-16
Identities = 35/67 (52%), Positives = 46/67 (68%)
Frame = +1
Query: 61 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 240
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 241 ILTLNTP 261
+L + P
Sbjct: 68 LLKIQRP 74
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 69.3 bits (162), Expect = 6e-13
Identities = 31/83 (37%), Positives = 47/83 (56%)
Frame = +3
Query: 261 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNSP 440
I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MFE+ P
Sbjct: 69 IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCP 128
Query: 441 AMYVAIQAVLSLYASGRTTGIVL 509
A Y+A Q + +ASG+ T ++
Sbjct: 129 ATYLAKQETCAAFASGKGTACLV 151
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 58 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 159
D+V+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 65.7 bits (153), Expect = 7e-12
Identities = 32/86 (37%), Positives = 53/86 (61%), Gaps = 2/86 (2%)
Frame = +3
Query: 258 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNS 437
P E +I+NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N+
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 438 PAMYVAIQAVLSLYASGR--TTGIVL 509
P++ I + S Y + + ++GIVL
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVL 170
Score = 37.1 bits (82), Expect = 0.003
Identities = 20/55 (36%), Positives = 33/55 (60%)
Frame = +1
Query: 4 ESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 168
E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 8 EPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 32.7 bits (71), Expect = 0.060
Identities = 17/75 (22%), Positives = 32/75 (42%)
Frame = +2
Query: 515 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEK 694
G+ SH +P+ G + R+ G + YL+K+ + SF +
Sbjct: 173 GNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHD 232
Query: 695 LCYVALDFEQEMATA 739
C+V+ D+ ++A A
Sbjct: 233 HCHVSSDYTHDIAHA 247
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.8 bits (136), Expect = 8e-10
Identities = 24/81 (29%), Positives = 48/81 (59%)
Frame = +3
Query: 267 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFENFNSPAM 446
+GII +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 447 YVAIQAVLSLYASGRTTGIVL 509
Y+ QAV +A+ ++T +++
Sbjct: 134 YLTKQAVCVAFANSKSTALIV 154
Score = 36.3 bits (80), Expect = 0.005
Identities = 18/57 (31%), Positives = 33/57 (57%)
Frame = +1
Query: 58 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 228
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 57.2 bits (132), Expect = 2e-09
Identities = 26/74 (35%), Positives = 47/74 (63%)
Frame = +2
Query: 509 DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIK 688
DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IK
Sbjct: 162 DSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIK 219
Query: 689 EKLCYVALDFEQEM 730
E +CYV+ +F+++M
Sbjct: 220 ESVCYVSQNFKEDM 233
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 47.2 bits (107), Expect = 3e-06
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 4/93 (4%)
Frame = +3
Query: 243 PHSEYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 410
P+ PI+ G + +W+ ++ W H Y+ L P + +PV L +RE T
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166
Query: 411 QIMFENFNSPAMYVAIQAVLSLYASGRTTGIVL 509
Q FE P +A + ++ LYA G G+V+
Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIGILHGLVI 199
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 35.9 bits (79), Expect = 0.006
Identities = 18/53 (33%), Positives = 29/53 (54%)
Frame = +1
Query: 118 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLNTPSSTVS 276
G+ PRA F ++ P H G+++ M KD G+E S +G + TP + +S
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEIS 552
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.32
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 318 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFENFNSPAM---YVAIQ 461
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 3.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 543 STKVTLCPTPSSVWTW 590
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC17D11.06 |spp2|pri2|DNA primase large subunit Spp2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 459
Score = 26.6 bits (56), Expect = 3.9
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
Frame = +1
Query: 649 HHHR*EGNRS*HQG---EALLCRPRLRAGDGHRCRFHS 753
H + EGNR ++G + +L P+L GD H C + +
Sbjct: 353 HTYGLEGNRKNYKGYNCQQILTGPQLGPGDAHGCPYRT 390
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.2
Identities = 16/82 (19%), Positives = 39/82 (47%), Gaps = 6/82 (7%)
Frame = +2
Query: 542 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF-----TTTAEREIVRDIKEKLCYV 706
+ EG +P++ ++++ G D+T MK+L + + T + + +K K C +
Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPYQDIDLKTPYDWSLANALKIKYCGL 367
Query: 707 A-LDFEQEMATAAASTPSRSLR 769
+ + ++ + + TP + R
Sbjct: 368 SEATYNVQLNSFFSRTPDKGTR 389
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 26.2 bits (55), Expect = 5.2
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +3
Query: 234 ERYPHSEYPIEHGIITNWDDM 296
E YP E+PI+ I W+ M
Sbjct: 1369 EHYPSGEFPIDRKFILYWEPM 1389
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.4 bits (53), Expect = 9.1
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +3
Query: 477 YASGRTTGIVLTPEMVSPTPYPSTKVT 557
Y G TT + T V+PTP PS VT
Sbjct: 22 YTPGFTTDVATT---VTPTPLPSANVT 45
>SPAC1002.02 |mug31||nucleoporin Pom34 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 229
Score = 25.4 bits (53), Expect = 9.1
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = +3
Query: 465 VLSLYASGRTTGIVLTPEMVSPTPYPSTKVTLCPTPSSVWTW 590
++ L G T+G LTP P PS K +P TW
Sbjct: 187 LMGLPEGGSTSGKHLTPPRYRPNFSPSRKAENVKSPVRSTTW 228
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,083,925
Number of Sequences: 5004
Number of extensions: 65470
Number of successful extensions: 276
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 270
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 373338084
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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