Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0686.Seq
(763 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 2e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 50 4e-07
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 38 0.002
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 30 0.41
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 29 0.72
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 2.2
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 2.2
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 27 2.9
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 3.9
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.7
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 6.7
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 6.7
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 25 8.9
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 57.2 bits (132), Expect = 2e-09
Identities = 34/89 (38%), Positives = 50/89 (56%), Gaps = 6/89 (6%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSD 422
D+DG I N+L SLG+ + EL +M+ E +G I+FT+ LT+ A +M +D
Sbjct: 24 DQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-DTD 82
Query: 423 EDDVVINAFKTFDEEGK--IDSERLRHAL 503
++ V AFK FD++G I E L H L
Sbjct: 83 NEEEVREAFKVFDKDGNGYITVEELTHVL 111
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 515 DKFSADEVHEAYDQMDIDDKGYIDTTKLIAMLTA 616
D + +EV EA+ D D GYI +L +LT+
Sbjct: 80 DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTS 113
Score = 25.4 bits (53), Expect = 8.9
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDQ 255
+ +Q+AEF+EAF L D+
Sbjct: 7 TDEQIAEFREAFSLFDR 23
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 50.0 bits (114), Expect = 4e-07
Identities = 30/85 (35%), Positives = 44/85 (51%), Gaps = 2/85 (2%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDV 434
D DG IG+ D++ SL + ASE ++ M + PIN LT + + S +D
Sbjct: 60 DGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRND- 118
Query: 435 VINAFKTFD--EEGKIDSERLRHAL 503
++ AF TFD + GKI +R AL
Sbjct: 119 LLEAFSTFDDTQSGKIPISTMRDAL 143
Score = 29.5 bits (63), Expect = 0.55
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +1
Query: 160 SRKAKRTGSNXXSMFSQKQVAEFKEAFQLMDQ 255
++ AKR S + + Q+ E KEAF L+D+
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDK 59
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 37.5 bits (83), Expect = 0.002
Identities = 22/87 (25%), Positives = 46/87 (52%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDV 434
DKDG+I + + + SLG ++ EL ++ E I+ + ++ +N++ E++
Sbjct: 21 DKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEE- 79
Query: 435 VINAFKTFDEE--GKIDSERLRHALMT 509
I AF+ FD++ G I++ + + T
Sbjct: 80 YIKAFRVFDKDNSGYIETAKFADYMKT 106
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 29.9 bits (64), Expect = 0.41
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMV 347
D G+IG +LR SLG S +E+DE++
Sbjct: 89 DATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 29.1 bits (62), Expect = 0.72
Identities = 25/71 (35%), Positives = 33/71 (46%)
Frame = -1
Query: 445 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 266
A I S SS+PP R S V+ A ++S+ FS P ES + S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326
Query: 265 PSLSGPLAGMP 233
PSL A +P
Sbjct: 327 PSLESGKAELP 337
>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 231
Score = 27.5 bits (58), Expect = 2.2
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = -1
Query: 340 SSSSFSDASLPRESKVARKSFLPMMPSLSGPLAGMP 233
SSSS S +S P + +RK P MP L L P
Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPELPSFLLAFP 38
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2
Query: 530 DEVHEAYDQMDIDDKGYID 586
+E EA+D D+ KGYID
Sbjct: 12 EEAEEAFDLFDVTHKGYID 30
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 27.1 bits (57), Expect = 2.9
Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 6/86 (6%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSD 422
DKD I ++LRA +LG A + E+ +++ + G + + + ++ D
Sbjct: 49 DKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIV-ERD 107
Query: 423 EDDVVINAFKTF--DEEGKIDSERLR 494
+ + AF+ F DE GKI LR
Sbjct: 108 PLEEIKRAFELFDDDETGKISLRNLR 133
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 26.6 bits (56), Expect = 3.9
Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Frame = -1
Query: 421 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 260
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 6.7
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +3
Query: 636 AVKPVMIPSLL*GLRRQPQSS-RPATVHSD*TSPYEPNF 749
AV V +PS++ + +QP SS PAT S P + +F
Sbjct: 1488 AVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLPPSQSSF 1526
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = +2
Query: 491 QARPHDWGDKFSADEVHEAYDQMDIDDKGYID 586
+A P ++E+ +D +D D+GYID
Sbjct: 323 EAVPFFMASHLDSEELARIWDTVDTQDRGYID 354
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 6.7
Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = -1
Query: 355 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 260
+SP++ +++ + P++S V+RKSF P P+
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 25.4 bits (53), Expect = 8.9
Identities = 17/43 (39%), Positives = 21/43 (48%), Gaps = 3/43 (6%)
Frame = +1
Query: 202 FSQKQVAEFKEAFQLMDQTRMASSART---TCAPPSTPWAGSR 321
FS+K + F A +DQ +AS T TC PS WA R
Sbjct: 28 FSEKSLLNFDPA-PWVDQHFVASPVSTKTATCPDPSWEWAWQR 69
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 25.4 bits (53), Expect = 8.9
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = -1
Query: 358 EASPTISSSSFSDASLPRESKVARKSFLPMMPSLSGPLAGMPP 230
E PTI SS +D+++PR V + S P +P+ P A PP
Sbjct: 135 EQQPTILSS--TDSNIPRPGTV-KSSASPFVPN-QNPSAPPPP 173
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,295,868
Number of Sequences: 5004
Number of extensions: 35841
Number of successful extensions: 152
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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