Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0686.Seq
(763 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12860.1 68417.m02014 calcium-binding protein, putative simil... 44 1e-04
At3g51920.1 68416.m05695 calmodulin-9 (CAM9) identical to calmod... 39 0.003
At2g41100.2 68415.m05077 touch-responsive protein / calmodulin-r... 37 0.013
At2g41100.1 68415.m05076 touch-responsive protein / calmodulin-r... 37 0.013
At4g04695.1 68417.m00689 calcium-dependent protein kinase, putat... 35 0.068
At1g05990.1 68414.m00627 calcium-binding protein, putative stron... 35 0.068
At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to calmod... 33 0.16
At3g03000.1 68416.m00295 calmodulin, putative similar to calmodu... 33 0.16
At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identica... 33 0.16
At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to ca... 32 0.36
At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to... 32 0.36
At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost i... 32 0.36
At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identica... 32 0.36
At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identica... 32 0.36
At3g50360.1 68416.m05507 caltractin / centrin identical to caltr... 31 1.1
At4g02540.1 68417.m00347 DC1 domain-containing protein contains ... 30 1.5
At4g35310.1 68417.m05019 calcium-dependent protein kinase, putat... 30 1.9
At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containi... 29 2.6
At4g20190.1 68417.m02952 hypothetical protein 29 2.6
At5g51600.1 68418.m06397 microtubule associated protein (MAP65/A... 29 3.4
At3g43480.1 68416.m04609 hypothetical protein hypothetical prote... 29 3.4
At3g43470.1 68416.m04608 hypothetical protein 29 3.4
At2g15185.1 68415.m01732 zinc knuckle (CCHC-type) protein-relate... 29 3.4
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 28 5.9
At2g44310.1 68415.m05513 calcium-binding EF hand family protein ... 28 7.8
At1g21550.1 68414.m02695 calcium-binding protein, putative conta... 28 7.8
>At4g12860.1 68417.m02014 calcium-binding protein, putative similar
to calcium-binding protein GI:6580549 from [Lotus
japonicus]
Length = 152
Score = 44.0 bits (99), Expect = 1e-04
Identities = 27/87 (31%), Positives = 47/87 (54%), Gaps = 6/87 (6%)
Frame = +3
Query: 261 DGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDED 428
DG I KN+L+ F S+G + E E++EM+ + G ++ + +L+ + +E+
Sbjct: 18 DGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKEEEE 77
Query: 429 DVVINAFKTFDE--EGKIDSERLRHAL 503
D + AF+ FD+ +G I E LR L
Sbjct: 78 D-MREAFRVFDQNGDGFITDEELRSVL 103
>At3g51920.1 68416.m05695 calmodulin-9 (CAM9) identical to
calmodulin 9 GI:5825602 from [Arabidopsis thaliana];
contains Pfam profile PF00036: EF hand
Length = 151
Score = 39.1 bits (87), Expect = 0.003
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 4/76 (5%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSD 422
D DG I K L S+G+ ++L +M+ + +G I F L + A S S
Sbjct: 23 DSDGFITKEKLTKVMKSMGKNPKAEQLQQMMSDVDIFGNGGITFDDFLYIMAQNTSQESA 82
Query: 423 EDDVVINAFKTFDEEG 470
D+ +I F+ FD +G
Sbjct: 83 SDE-LIEVFRVFDRDG 97
>At2g41100.2 68415.m05077 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 235
Score = 37.1 bits (82), Expect = 0.013
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 4/53 (7%)
Frame = +3
Query: 261 DGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRM 407
DG I N+LR T SLG ++ EL +M+ EA G I+F++ + + +M
Sbjct: 115 DGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKM 167
>At2g41100.1 68415.m05076 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 324
Score = 37.1 bits (82), Expect = 0.013
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 4/53 (7%)
Frame = +3
Query: 261 DGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRM 407
DG I N+LR T SLG ++ EL +M+ EA G I+F++ + + +M
Sbjct: 204 DGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKM 256
>At4g04695.1 68417.m00689 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Lycopersicon esculentum]
gi|19171502|emb|CAC87494; contains protein kinase
domain, Pfam:PF00069; contains EF hand domain
(calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 484
Score = 34.7 bits (76), Expect = 0.068
Identities = 26/89 (29%), Positives = 44/89 (49%), Gaps = 6/89 (6%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSD 422
DK G I +L+ LG S+ E+++++ A +G I+ + ++ +R D
Sbjct: 347 DKSGTITLEELKTGLTRLGSNLSKTEVEQLMEAADVDGNGTIDIDEFISATMHRYR--LD 404
Query: 423 EDDVVINAFKTFDE--EGKIDSERLRHAL 503
DD V AF+ FD+ +G I E L A+
Sbjct: 405 RDDHVYQAFQHFDKDNDGHITKEELEMAM 433
>At1g05990.1 68414.m00627 calcium-binding protein, putative strong
similarity to calcium-binding protein [Lotus japonicus]
GI:18413495; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 150
Score = 34.7 bits (76), Expect = 0.068
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 4/74 (5%)
Frame = +3
Query: 261 DGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGGSDED 428
DG I +L T SLG +KEL +M+ + G ++ + L+ M +E+
Sbjct: 18 DGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGELYKTIMDEEDEEE 77
Query: 429 DVVINAFKTFDEEG 470
+ + AF FD+ G
Sbjct: 78 EDMKEAFNVFDQNG 91
>At5g21274.1 68418.m02533 calmodulin-6 (CAM6) identical to
calmodulin-6 SP:Q03509 from [Arabidopsis thaliana];
contains Pfam profile: PF00036 EF hand
Length = 149
Score = 33.5 bits (73), Expect = 0.16
Identities = 17/48 (35%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG S++E+DEM+ EA G IN+ + +
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLSDEEVDEMIREADVDGDGQINYEEFV 143
>At3g03000.1 68416.m00295 calmodulin, putative similar to calmodulin
SP:P04352 from [Chlamydomonas reinhardtii]; contains
Pfam profile: PF00036 EF hand (4 copies)
Length = 165
Score = 33.5 bits (73), Expect = 0.16
Identities = 23/89 (25%), Positives = 45/89 (50%), Gaps = 6/89 (6%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSD 422
+KDG + + +L + SLG S+ +LD ++ +A +G + F++ + L +
Sbjct: 31 NKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEFSEFVALVEPDLVKCPY 90
Query: 423 EDDVVINAFKTFDEEGK--IDSERLRHAL 503
DD + F+ FD +G I + L H++
Sbjct: 91 TDDQLKAIFRMFDRDGNGYITAAELAHSM 119
>At2g27030.3 68415.m03247 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 181
Score = 33.5 bits (73), Expect = 0.16
Identities = 17/57 (29%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSG 413
D++G I +LR +LG +++E+DEM+ EA G IN+ + + + + G
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFVKVMMAKRRG 152
>At3g56800.1 68416.m06317 calmodulin-2/3/5 (CAM3) identical to
calmodulin GI:474183 from [Arabidopsis thaliana]; almost
identical to calmodulin-2/3/5 SP:P25069 [Arabidopsis
thaliana]
Length = 149
Score = 32.3 bits (70), Expect = 0.36
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 143
>At3g43810.1 68416.m04682 calmodulin-7 (CAM7) almost identical to
calmodulin GI:16227 from [Arabidopsis thaliana],
SP|P59220 Calmodulin-7 {Arabidopsis thaliana}
Length = 149
Score = 32.3 bits (70), Expect = 0.36
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQINYEEFV 143
>At2g41110.1 68415.m05078 calmodulin-2/3/5 (CAM2) (CAL1) almost
identical to Calmodulin-2/3/5 SP:P25069 from
[Arabidopsis thaliana]
Length = 149
Score = 32.3 bits (70), Expect = 0.36
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 143
>At2g27030.2 68415.m03246 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 113
Score = 32.3 bits (70), Expect = 0.36
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 60 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 107
>At2g27030.1 68415.m03245 calmodulin-2/3/5 (CAM5) (TCH1) identical
to calmodulin GI:474183 from [Arabidopsis thaliana],
SP|P25069 Calmodulin-2/3/5 {Arabidopsis thaliana}
Length = 149
Score = 32.3 bits (70), Expect = 0.36
Identities = 16/48 (33%), Positives = 28/48 (58%), Gaps = 4/48 (8%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLL 386
D++G I +LR +LG +++E+DEM+ EA G IN+ + +
Sbjct: 96 DQNGFISAAELRHVMTNLGEKLTDEEVDEMIKEADVDGDGQINYEEFV 143
>At3g50360.1 68416.m05507 caltractin / centrin identical to
caltractin; centrin GI:3688162 from [Arabidopsis
thaliana]
Length = 169
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGS 419
DK+G I +D++ LG ++ E+ EMV EA G +N + + + GG+
Sbjct: 111 DKNGKISPDDIKRMAKDLGENFTDAEIREMVEEADRDRDGEVNMDEFMRMMRRTAYGGN 169
>At4g02540.1 68417.m00347 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 822
Score = 30.3 bits (65), Expect = 1.5
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = -1
Query: 439 ITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFS-DASL 311
+ S+ SEPP+M+LA+ +K + L+ + P + S + DA L
Sbjct: 88 LVESTYSEPPEMKLASLIKKLMPLIDTQIPPIFTGKSLTYDAKL 131
>At4g35310.1 68417.m05019 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 556
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 467 GKDRF*KAQARPHDWGDKFSADEVHEAYDQMDIDDKGYIDTTKLIA 604
G F + +A +G E+H+ D D+D+ G ID ++ IA
Sbjct: 416 GAITFDELKAGLRKYGSTLKDTEIHDLMDAADVDNSGTIDYSEFIA 461
>At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 574
Score = 29.5 bits (63), Expect = 2.6
Identities = 20/49 (40%), Positives = 26/49 (53%)
Frame = +3
Query: 321 SEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEE 467
+E+ L M G P N T LL A R++G +DE + INA K D E
Sbjct: 453 AEEVLQMMRGCGVKPENSTFLLLAEAWRVAGLTDESNKAINALKCKDIE 501
>At4g20190.1 68417.m02952 hypothetical protein
Length = 389
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +3
Query: 411 GGSDEDDVVINAFKTFDEEGKIDSERLRHALMTGATSS 524
GG+D+DD V AF FD+E +D E ++ L T + S
Sbjct: 306 GGNDQDDPVSAAF-VFDKEPNLDKE-IKGVLKTSGSKS 341
>At5g51600.1 68418.m06397 microtubule associated protein
(MAP65/ASE1) family protein low similarity to SP|P50275
Anaphase spindle elongation protein {Saccharomyces
cerevisiae}, protein regulating cytokinesis 1 (PRC1)
[Homo sapiens] GI:2865521; contains Pfam profile
PF03999: Microtubule associated protein (MAP65/ASE1
family)
Length = 707
Score = 29.1 bits (62), Expect = 3.4
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -3
Query: 401 VGEQSQELSEVDGARSLTDHLIELL 327
VG+ + LS+ +G RSL+DH IE L
Sbjct: 206 VGQVNPTLSDPEGPRSLSDHTIEKL 230
>At3g43480.1 68416.m04609 hypothetical protein hypothetical protein
At2g15180 - Arabidopsis thaliana, EMBL:AC006298
Length = 161
Score = 29.1 bits (62), Expect = 3.4
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 679 DASRSHHARPRFTRIKHHH 735
D +SHH +P+ +R+K HH
Sbjct: 116 DVPKSHHFQPKLSRLKTHH 134
>At3g43470.1 68416.m04608 hypothetical protein
Length = 458
Score = 29.1 bits (62), Expect = 3.4
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 679 DASRSHHARPRFTRIKHHH 735
D +SHH +P+ +R+K HH
Sbjct: 413 DVPKSHHFQPKLSRLKTHH 431
>At2g15185.1 68415.m01732 zinc knuckle (CCHC-type) protein-related
supported by tandem duplication of zinc knuckle (CCHC
type) familyprotein (GI:4585917) (TIGR_Ath1:At2g15180)
[Arabidopsis thaliana]
Length = 191
Score = 29.1 bits (62), Expect = 3.4
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +1
Query: 679 DASRSHHARPRFTRIKHHH 735
D +SHH +P+ +R+K HH
Sbjct: 146 DVPKSHHFQPKLSRLKTHH 164
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +3
Query: 264 GIIGKNDLRATFDSLGRLASEKELDEMVGEA 356
G + D+R T SLG+ S +E+ E+V A
Sbjct: 1288 GYVRVEDMRVTIHSLGKFLSHREVKELVQSA 1318
>At2g44310.1 68415.m05513 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 142
Score = 27.9 bits (59), Expect = 7.8
Identities = 14/59 (23%), Positives = 29/59 (49%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDD 431
+KDG++ +++LR F+S+ L S +D + + + + F SG D ++
Sbjct: 35 NKDGVLSRSELRKAFESMRLLESHFGVDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEE 93
>At1g21550.1 68414.m02695 calcium-binding protein, putative contains
similarity to calcium-binding protein GB:CAB63264
GI:6580549 from [Lotus japonicus]
Length = 155
Score = 27.9 bits (59), Expect = 7.8
Identities = 27/96 (28%), Positives = 47/96 (48%), Gaps = 13/96 (13%)
Frame = +3
Query: 255 DKDGIIGKNDLRATFDSLGRLA-SEKELDEMVGEASGPINFTQLLTLFANRM--SGGS-- 419
++DG++ ++L D LG + EL+ +VG+ S ++ + L + + + S GS
Sbjct: 21 NQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQS--LDLDEFLRFYYDAVLDSKGSKK 78
Query: 420 ------DEDDVVINAFKTFD--EEGKIDSERLRHAL 503
D D+ + AF FD +G I +E LR L
Sbjct: 79 NIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVL 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,697,149
Number of Sequences: 28952
Number of extensions: 206022
Number of successful extensions: 840
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 840
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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