Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0685.Seq
(674 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 142 3e-34
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 138 3e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 134 5e-32
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 120 8e-28
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 71 9e-13
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 70 2e-12
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 58 6e-09
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.002
SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06) 31 0.85
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.85
SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.85
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.6
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.5
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.0
SB_41041| Best HMM Match : PDZ (HMM E-Value=1.3e-40) 28 7.9
SB_16515| Best HMM Match : Extensin_2 (HMM E-Value=0.12) 28 7.9
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 142 bits (343), Expect = 3e-34
Identities = 65/89 (73%), Positives = 77/89 (86%)
Frame = +2
Query: 242 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 421
P+ IFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 422 VLTKMKETAEAYLGKTVQNAVITVPAYFN 508
VL KMKETAEAYLG+ V +AVITVPAYFN
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFN 151
Score = 131 bits (317), Expect = 4e-31
Identities = 59/66 (89%), Positives = 63/66 (95%)
Frame = +3
Query: 57 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 236
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 237 MNPNNT 254
+NP NT
Sbjct: 61 LNPENT 66
Score = 99.5 bits (237), Expect = 2e-21
Identities = 46/53 (86%), Positives = 51/53 (96%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTF 669
SQRQATKDAGTI+GLNVLR+INEPTAAA+AYGLDK +GE+NVLIFDLGGGTF
Sbjct: 153 SQRQATKDAGTIAGLNVLRVINEPTAAALAYGLDKSLSGEKNVLIFDLGGGTF 205
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 138 bits (335), Expect = 3e-33
Identities = 61/89 (68%), Positives = 74/89 (83%)
Frame = +2
Query: 242 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 421
P+ +FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 422 VLTKMKETAEAYLGKTVQNAVITVPAYFN 508
VLTKMKETAEAYLG V +AV+TVPAYF+
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFH 151
Score = 136 bits (329), Expect = 2e-32
Identities = 63/66 (95%), Positives = 64/66 (96%)
Frame = +3
Query: 57 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 236
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 237 MNPNNT 254
MNP NT
Sbjct: 61 MNPENT 66
Score = 98.3 bits (234), Expect = 5e-21
Identities = 48/53 (90%), Positives = 50/53 (94%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTF 669
SQRQATKDAGTI+GLNVLRIINEPTAAAIAYGLDKK ERNVLI+DLGGGTF
Sbjct: 153 SQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGVERNVLIYDLGGGTF 205
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 134 bits (325), Expect = 5e-32
Identities = 61/66 (92%), Positives = 63/66 (95%)
Frame = +3
Query: 57 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 236
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 237 MNPNNT 254
MNP NT
Sbjct: 61 MNPTNT 66
Score = 116 bits (280), Expect = 1e-26
Identities = 65/114 (57%), Positives = 74/114 (64%), Gaps = 25/114 (21%)
Frame = +2
Query: 242 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 412
P IFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 413 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFN 508
SSMVL KMKETAEAYLG V +AV+TVPAYFN
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFN 176
Score = 99.1 bits (236), Expect = 3e-21
Identities = 49/53 (92%), Positives = 49/53 (92%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTF 669
SQRQATKDAG ISGLNVLRIINEPTAAAIAYGLDKK ERNVLIFDLGGGTF
Sbjct: 246 SQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKVGQERNVLIFDLGGGTF 298
Score = 56.8 bits (131), Expect = 1e-08
Identities = 26/34 (76%), Positives = 30/34 (88%)
Frame = +2
Query: 407 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFN 508
++SSMVL KMKETAEAYLG V +AV+TVPAYFN
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFN 244
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 120 bits (290), Expect = 8e-28
Identities = 57/97 (58%), Positives = 73/97 (75%)
Frame = +2
Query: 242 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 421
P+ +FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 422 VLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKPQK 532
VLTKMKETAEAYLGK V +AV+TVPAYFN + + K
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATK 857
Score = 104 bits (249), Expect = 8e-23
Identities = 48/61 (78%), Positives = 54/61 (88%), Gaps = 1/61 (1%)
Frame = +3
Query: 75 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 251
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 252 T 254
T
Sbjct: 764 T 764
Score = 91.1 bits (216), Expect = 8e-19
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTF 669
+QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK GE+N+L+FDLGGGTF
Sbjct: 851 AQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK-EGEKNILVFDLGGGTF 902
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 70.9 bits (166), Expect = 9e-13
Identities = 35/81 (43%), Positives = 51/81 (62%), Gaps = 1/81 (1%)
Frame = +2
Query: 266 KRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 442
KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 443 TAEAYLGKTVQNAVITVPAYF 505
TAE L V + VI+VP+Y+
Sbjct: 187 TAEIALKTKVTDCVISVPSYY 207
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +3
Query: 75 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 230
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 42.3 bits (95), Expect = 3e-04
Identities = 21/51 (41%), Positives = 32/51 (62%), Gaps = 3/51 (5%)
Frame = +1
Query: 514 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKG---TGERNVLIFDLG 657
QR+ DA +GLN LR++N+ TA ++AYG+ K+ RNV+ D+G
Sbjct: 211 QRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQDLPTDKPRNVVFVDIG 261
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 69.7 bits (163), Expect = 2e-12
Identities = 31/66 (46%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +3
Query: 63 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 236
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 237 MNPNNT 254
+NP NT
Sbjct: 90 INPKNT 95
Score = 67.3 bits (157), Expect = 1e-11
Identities = 32/51 (62%), Positives = 40/51 (78%)
Frame = +1
Query: 514 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGT 666
QR AT A +++G+ VLR+INEPTAAA+AYGL KK G NV++ DLGGGT
Sbjct: 184 QRNATVKAASLAGITVLRVINEPTAAALAYGLHKK-EGVANVMVVDLGGGT 233
Score = 56.4 bits (130), Expect = 2e-08
Identities = 36/105 (34%), Positives = 53/105 (50%), Gaps = 1/105 (0%)
Frame = +2
Query: 242 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSS 418
P+ I+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 419 MVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKPQKMQVPSQA 553
V+ K++ AE L TV AV++VPA F+ +K + ++ S A
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEFD-IKQRNATVKAASLA 195
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 58.0 bits (134), Expect = 6e-09
Identities = 27/57 (47%), Positives = 41/57 (71%)
Frame = +2
Query: 362 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKPQK 532
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FN + + K
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATK 58
Score = 48.0 bits (109), Expect = 7e-06
Identities = 36/68 (52%), Positives = 39/68 (57%), Gaps = 26/68 (38%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVL------------------------RIINEPTAAAIAYGLDKK 618
+QRQATKDAGTI+GL+VL RIINEPTAAAIAYGLDK
Sbjct: 52 AQRQATKDAGTIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDKA 111
Query: 619 --GTGERN 636
G ERN
Sbjct: 112 RGGKAERN 119
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +3
Query: 126 GKVEIIANDQGNRTTPSYVAFTDTERLI 209
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/51 (35%), Positives = 29/51 (56%)
Frame = +2
Query: 356 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFN 508
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFN 117
Score = 37.9 bits (84), Expect = 0.007
Identities = 20/56 (35%), Positives = 35/56 (62%), Gaps = 4/56 (7%)
Frame = +1
Query: 511 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK----GTGERNVLIFDLGGGT 666
++R+A A + GLNVL+I+N TA A+ YGL ++ T E++ + +D+G +
Sbjct: 119 AERRALLRAAELVGLNVLQIMNSNTAVALNYGLFQQKSFNDTLEKHFMFYDMGASS 174
>SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06)
Length = 286
Score = 31.1 bits (67), Expect = 0.85
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Frame = +3
Query: 456 ISAKLCRM-QLSRFPRTSILSKTSHKR-CRYHLRLERSPNH 572
+SAKLC + LS+ R HKR C Y +++E SP H
Sbjct: 198 LSAKLCHVVTLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 238
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.85
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +3
Query: 135 EIIANDQGNRTTPSYVAFT-DTERL 206
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 743
Score = 31.1 bits (67), Expect = 0.85
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Frame = +3
Query: 456 ISAKLCRM-QLSRFPRTSILSKTSHKR-CRYHLRLERSPNH 572
+SAKLC + LS+ R HKR C Y +++E SP H
Sbjct: 655 LSAKLCHVITLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 695
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 243 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 145
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.5
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 3 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 89
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 6.0
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -3
Query: 450 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRP 277
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
>SB_41041| Best HMM Match : PDZ (HMM E-Value=1.3e-40)
Length = 933
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +2
Query: 353 KPKIKVA-YKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNTLKDKPQ 529
+P K A Y +D+T E + + LT + ++ NAV P Y++ L+DKP
Sbjct: 435 RPADKQAEYLEDDETDGFESGALLNLTPARSRNPVQRNESYCNAVSRSPGYYSPLRDKPA 494
Query: 530 K 532
+
Sbjct: 495 R 495
>SB_16515| Best HMM Match : Extensin_2 (HMM E-Value=0.12)
Length = 917
Score = 27.9 bits (59), Expect = 7.9
Identities = 28/77 (36%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
Frame = +2
Query: 242 PQQ-HIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 418
PQQ H+ DA+ IGR A + DM F + GG P+ K K ++KT + V+S
Sbjct: 840 PQQFHVSDARSRIGRLLIKAAKKNDMVTSHF---TKGG-PRNK--GKEDEKTL--DRVAS 891
Query: 419 MVLTKMKETAEAYLGKT 469
M+ T++ T + GKT
Sbjct: 892 MIATRLLHTNKT--GKT 906
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,793,217
Number of Sequences: 59808
Number of extensions: 523733
Number of successful extensions: 1687
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1678
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1733301648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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