Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= pg--0569.Seq
(786 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 155 9e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 45 1e-05
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 32 0.081
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.11
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 1.00
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 27 2.3
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma... 27 4.0
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 26 5.3
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 25 9.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQIS GY+PVLDCHTAHIACKF
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKF 370
Score = 120 bits (288), Expect = 3e-28
Identities = 58/94 (61%), Positives = 70/94 (74%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H + +E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIKAV G KVTKAA K
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVK 455
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQIS GY+PVLDCHTAHIACKF
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKF 370
Score = 120 bits (288), Expect = 3e-28
Identities = 58/94 (61%), Positives = 70/94 (74%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H + +E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIKAV G KVTKAA K
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVK 455
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 155 bits (375), Expect = 9e-39
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 581
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 580 HPGQISNGYTPVLDCHTAHIACKF 509
HPGQIS GY+PVLDCHTAHIACKF
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKF 370
Score = 120 bits (288), Expect = 3e-28
Identities = 58/94 (61%), Positives = 70/94 (74%), Gaps = 1/94 (1%)
Frame = -2
Query: 533 HCPH-CLQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 357
H H + +E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 356 FAVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEK 255
FAVRDMRQTVAVGVIKAV G KVTKAA K
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVK 455
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 45.2 bits (102), Expect = 1e-05
Identities = 23/74 (31%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
Frame = -2
Query: 518 LQISEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 339
+ +++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD
Sbjct: 590 VSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQ 648
Query: 338 RQTVAVG-VIKAVN 300
TVAVG V+K ++
Sbjct: 649 GTTVAVGKVVKILD 662
Score = 41.1 bits (92), Expect = 2e-04
Identities = 22/70 (31%), Positives = 36/70 (51%)
Frame = -3
Query: 736 EALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 557
E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ G
Sbjct: 519 EEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTG 576
Query: 556 YTPVLDCHTA 527
Y+ V+ HTA
Sbjct: 577 YSCVMHIHTA 586
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 32.3 bits (70), Expect = 0.081
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -3
Query: 760 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 650
V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 298 VTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.11
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -2
Query: 401 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 309
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 1.00
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +1
Query: 424 MAASPDLMDFGLTSVDLPVRRSTFSLISEICRQCGQCGNPRLVCNRLRFDQDG 582
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 27.5 bits (58), Expect = 2.3
Identities = 25/87 (28%), Positives = 43/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 253 GFSAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTM 426
G S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++
Sbjct: 181 GTSTDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASL 239
Query: 427 AASPDLMDFGLTSVDLPVRRSTFSLIS 507
++S L +TS PV S+ SL S
Sbjct: 240 SSSSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.0
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = -2
Query: 578 SWSNLKRLHTSLGLPHCPHCLQISEIKEKVDRRTGKSTEVNPKSIKS 438
S +N LH + L + +ISEIKE D + E+ K ++S
Sbjct: 792 SEANAPELHNPVNLNYDAQLSKISEIKENYDELLTRYRELEGKFLES 838
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 665 QFLDGHVLYVETYIVSRYSFLESFV 739
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 26.2 bits (55), Expect = 5.3
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +3
Query: 414 QVDNGCISRLDGFWVDFSRFTSTTVNFFFDF*NLQAMWAVWQSKTGV 554
+V C + ++FS T+ T N F A ++VW+ ++G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 25.4 bits (53), Expect = 9.3
Identities = 11/43 (25%), Positives = 19/43 (44%)
Frame = +1
Query: 436 PDLMDFGLTSVDLPVRRSTFSLISEICRQCGQCGNPRLVCNRL 564
P ++DF + ++ V+ + CG PR VC+ L
Sbjct: 279 PSMIDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGL 321
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,333,913
Number of Sequences: 5004
Number of extensions: 68508
Number of successful extensions: 187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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